【倉鼠腎成纖維細胞 YXK-21】齊一生物提供多國品牌細胞產(chǎn)品,公司常年備用細胞現(xiàn)貨,能滿足全國客戶對細胞研究的需求。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

選擇齊一生物的原因：

1.供貨及時。我們訂貨周期短，運輸時間短，在*短時間內(nèi)為您提供所需產(chǎn)品。

2.價格優(yōu)惠：美國ATCC的中國代理商，沒有中間環(huán)節(jié)。在國內(nèi)我們保證價格。

3.服務保障：我們擁有專用的技術指導團隊，對每售出的一件產(chǎn)品，提供全面的售前、售中和售后服務。

4.產(chǎn)品種類齊全：25個系統(tǒng)共150多種細胞以及各類菌株，滿足不同客戶的要求。細胞經(jīng)過嚴格的質(zhì)控，純度可達98％。常用產(chǎn)品備有現(xiàn)貨。其產(chǎn)品類型如下：消化系統(tǒng)細胞株，呼吸系統(tǒng)細胞株，泌尿系統(tǒng)細胞株生殖系統(tǒng)細胞株 血液循環(huán)系統(tǒng) 內(nèi)皮細胞細胞株 神經(jīng)系統(tǒng)及其他細胞株，動物細胞株和菌株

5.對于部分難培養(yǎng)的細胞我公司可代復蘇細胞，并提供技術支持。

6.經(jīng)驗豐富：我公司是國內(nèi)專業(yè)化的細胞銷售公司，具有十余年細胞產(chǎn)品銷售經(jīng)驗，有專業(yè)的銷售團隊和技術人員。齊一生物公司的每一位員工都將竭誠為您服務。

7.質(zhì)量保證，保證售后

8. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【倉鼠腎成纖維細胞 YXK-21】 齊一生物公司專業(yè)代理ScienCell簡介ScienCell Research Laboratories (ScienCell) is a biotechnology company whose mission is the research ａnd development of cell ａnd cell-related products for experimental ａnd therapeutic use. The ScienCell technology provides a variety of high quality normal human ａnd animal cells, cell culture media ａnd reagents, medium supplements, cell-derived RNA, cDNA ａnd proteins for the research community. Scientists at ScienCell are also studying ａnd developing cell therapeutic strategies that will significantly improve the quality of life by achieving the cell＇s ability to combat human degenerative disorders. We are based in San Diego, California ａnd were established in 1999.

【倉鼠腎成纖維細胞 YXK-21】美國ATCC簡介：美國菌種保藏中心， 又稱美國模式菌種收集中心(ATCC)，是位于馬里蘭洲洛克菲勒的一家私營的，非贏利性組織。目前它可以提供各種動植物細胞、標準品、菌株達兩萬多種。ATCC成立于1925年，是世界上的生物資源中心，由美國14家生化、醫(yī)學類行業(yè)協(xié)會組成的理事會負責管理，是一家全球性、非盈利生物標準品資源中心。ATCC向全球發(fā)布其獲取、鑒定、保存及開發(fā)的生物標準品，推動科學研究的驗證、應用及進步 ATCC可以提供以下類別生物標準品：細胞株（3000多種）；菌株（15000多種）；動植物病毒株（2500多種）以及重組物質(zhì)等。

現(xiàn)已成為可信賴的活體微生物、細胞系等獲得、保存和發(fā)放的國家資源中心。ATCC有29,000多種不同品系可靠的動物細胞和微生物培養(yǎng)體，它能滿足各科學團體對可信賴品系的需要。僅1981年就有39,000多培養(yǎng)物發(fā)放給全世界的科學工作者，并成功地用冷凍和冷凍干燥的方法保存各種細胞品系。 ATCC是一個非(學會單位)無利潤的公司，由各科學組織推選成員組成理事會進行管理�，F(xiàn)有工作人員125人。參加的科學團體有：美國免疫協(xié)會、美國生物化學會、美國細胞生物學會、美國微生物學會、美國動物學會、美國遺傳學會、原生動物學會和組織培養(yǎng)協(xié)會等16個學術組織。在ATCC可利用各種材料以及工具和設備進行廣泛的研究。主要研究項目有：酶聯(lián)免疫吸附分析技術(ELISA)，真核細胞轉錄的調(diào)控，雜交瘤、淋巴類和髓類以及其他細胞系的建立，人突變細胞系的基因蛋白質(zhì)，借助計算機進行鑒別分析，培養(yǎng)物的特征鑒別和保存技術的改進,關于化學致癌、致畸和致突變的。

【倉鼠腎成纖維細胞 YXK-21】齊一生物提醒接收到細胞注意事項

1. 接收到細胞，肉眼觀察細胞培養(yǎng)基顏色，顯微鏡觀察細胞生長情況，并對細胞進行不同倍數(shù)拍照（建議收到時的培養(yǎng)瓶拍一張照片，顯微鏡拍收到時的細胞100X,200X各一張）。

2.用75%的酒精消毒（建議配置75%的酒精的水是滅菌過的）。

3.嚴格無菌操作，打開細胞培養(yǎng)瓶。

4.將細胞培養(yǎng)瓶內(nèi)的培養(yǎng)基用吸管完全吸出（嚴禁直接傾倒），放入另一無菌容器中（建議換新的培養(yǎng)基培養(yǎng)細胞）。

5.用PBS 2-3ml/次輕輕洗細胞表面，洗3-4次遍，加入0.05%胰酶-EDTA1-1.5ml,顯微鏡下觀察細胞變圓，輕輕拍打培養(yǎng)瓶直到細胞脫落，加入終止液終止。

6.1000rpm，5min離心，重懸細胞。

7.換新的細胞培養(yǎng)瓶，37℃，5%CO2培養(yǎng)。【倉鼠腎成纖維細胞 YXK-21】

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

74182RNeasy 96 Kit (12)For 12 x 96 total RNA preps: 12 RNeasy 96 Plates, Elution Microtubes CL (1.2 ml), Caps, RNase-free Reagents ａnd BuffersCNY

74136RNeasy Plus Mini Kit (250)CNY

28004MinElute PCR Purification Kit (50)50 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)CNY

74124RNeasy Protect Mini Kit (50)RNAlater RNA Stabilization Reagent (50 ml), 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd BuffersCNY

19588Elution Microtubes CL (24 x 96)CNY

19133QIAGEN Proteinase K (10)10 ml (>600 mAU/ml, solution)CNY

19076Buffer ATL200 ml Tissue Lysis Buffer for 1000 prepsCNY

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12961QIAGEN Plasmid Plus Maxi Sample Kit (5)QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction.CNY

12243QIAfilter Plasmid Midi Kit (25)25 QIAGEN-tip 100, Reagents, Buffers, 25 QIAfilter Midi CartridgesCNY

12123QIAGEN Plasmid Mini Kit (25)25 QIAGEN-tip 20, Reagents, BuffersCNY

133276NeXtalStock Spermine (100)CNY

4503065artus CMV LC PCR Kit (96) CECNY

6000-5000Specimen Collection TubesCNY

5000-1430MST Vortexer II - FoamFeetAssembly, 110VCNY

5128-1220Specimen Transport Medium (STM) CECNY

5123-1220Female Swab Specimen Collection KitTMCNY

5100-1400IVDhc2 Sample Conversion KitCNY

EA11424EASYartus BKV RG_IICNY

5050-1075 V.1CD Rom Manual, MST Vortexer 2CNY

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BR-02DxS B-RAF Mutation Test KitCNY

9240729Tubing, 3/8"OD , Red, APCNY

9240719Cable Carrier, Capper, Elevator, APCNY

9240701O-Ring, Shaker, Lower Body, AP96/196CNY

9240696Fitting, Barbed, 1/4" ID tube, APCNY

9243651Comprehen Valid., QIAgility"""The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation. 

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   For planning purposes ａnd to ensure a smooth process, the Customer is expected to have all materials on-hand ａnd inventoried one (1) week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """CNY

218076miScript SYBR Green PCR Kit (2000)CNY

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