產(chǎn)品展示
人乳鐵傳遞蛋白乳鐵蛋白抗體IgG(LFLTFAb-Ig)ELISA試劑盒
點擊次數(shù):10發(fā)布時間:2016/6/7 20:57:48

更新日期:2016/6/29 8:33:04
所 在 地:中國大陸
產(chǎn)品型號:
優(yōu)質(zhì)供應(yīng)
詳細內(nèi)容
1.購買我公司任意ELISA試劑盒指標(biāo)享受免費代測,并提供技術(shù)指導(dǎo)服務(wù)!
二、實驗材料與試劑配制:
1. 儀器與材料:酶標(biāo)儀(使用前預(yù)熱30分鐘),微量加液器、吸頭、蒸餾水或去離子水,濾紙。
2. 緩沖液使用:加5ul 的緩沖液于50ul 的樣品中,如果樣品量不夠或者不確定,緩沖液和樣品的混合比例不要小于1:10即可混勻,靜置1小時備用(如果標(biāo)本是血清或者血漿,此步驟忽略)。
3. 洗液的配制:按1:100的比例配制洗液備用。
齊一生物銷售:021-6034 8496;181214 53965;173021 04490
【人乳鐵傳遞蛋白乳鐵蛋白抗體IgG(LFLTFAb-Ig)ELISA試劑盒】三. 仔細閱讀說明書
1.檢查試劑盒標(biāo)簽上的有效期。如果超過有效期,請勿使用。
2. 按說明書確定所有試劑齊全(數(shù)量、體積)
3. 標(biāo)本制備要規(guī)范,每份標(biāo)本體積要按2-3個復(fù)孔以上的量制備(貯存),盡量分裝做備份。
4.短期內(nèi)無法實驗者,注意低溫保存
5.準(zhǔn)備好所有實驗額外所需物品,(比如移液器,試管,清洗器,酶標(biāo)儀)
6. 按說明書將所用試劑平衡至室溫,根據(jù)檢測標(biāo)本數(shù)量確定所需試劑的量
7.檢查試劑盒內(nèi)每種試劑的貯存條件,保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲。 【人乳鐵傳遞蛋白乳鐵蛋白抗體IgG(LFLTFAb-Ig)ELISA試劑盒】
8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液(比如沉淀或變色)。有些試劑,比如標(biāo)準(zhǔn)品稀釋液或者檢測稀釋液,可能在設(shè)計時就含有沉淀物。所有試劑在滴入酶標(biāo)板之前,必需充分混勻。而由于沉淀物的特性,在加入酶標(biāo)板之前,必需不停攪拌。
9.保證充分的孵育時間和溫度。
10.切勿使用不同生產(chǎn)批號的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。
11.在混合或溶解蛋白溶液時,避免泡沫產(chǎn)生。
12.在實驗開始之前,安排好實驗流程。在實驗開始之前,清潔工作臺。
13.若有問題,應(yīng)與我公司或代理商的技術(shù)支持聯(lián)系
【人乳鐵傳遞蛋白乳鐵蛋白抗體IgG(LFLTFAb-Ig)ELISA試劑盒】四、客戶須知
齊一生物銷售:021-6034 8496;181214 53965;173021 04490
1.在收集標(biāo)本前都必須有一個完整的計劃,必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測,對收集后當(dāng)天就進行檢測的標(biāo)本,及時儲存在4℃?zhèn)溆,如有特殊原因需要周期收集?biāo)本,請造模取材后,將標(biāo)本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差,蛋白極易降解,直接影響實驗質(zhì)量,所以避免反復(fù)凍融。部分標(biāo)本也可加入適當(dāng)防腐劑,激素類標(biāo)本需添加抑肽酶。
2.試劑盒代測標(biāo)本的客戶取材前須向我司銷售人員索要說明書,具體操作注意事項請與我司技術(shù)人員溝通。
3.液體類標(biāo)本:標(biāo)本必須為液體,不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細胞培養(yǎng)上清、組織勻漿等。
4.購買前請?zhí)崆案嬖V我們:您樣本的種屬、樣本數(shù)量、待測指標(biāo)及其它特殊要求。
【人乳鐵傳遞蛋白乳鐵蛋白抗體IgG(LFLTFAb-Ig)ELISA試劑盒】五、代測數(shù)據(jù)詳細內(nèi)容
1、ELISA標(biāo)準(zhǔn)曲線;
2、實驗具體詳細步驟;
3、完整的實驗報告(試劑耗材,實驗方法,實驗結(jié)果及結(jié)果說明);
4、實驗進行階段,我公司客服人員會及時向您匯報實驗進程。
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