【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準備試劑，樣品和標準品

2.加入準備好的樣品和標準品，37℃反應30分鐘

3.洗板5次，加入酶標試劑，37℃反應30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計算
齊一生物供應ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標本前都必須有一個完整的計劃。

【大鼠Hepassocin（HPS）ELISA試劑盒】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【大鼠Hepassocin（HPS）ELISA試劑盒】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【大鼠Hepassocin（HPS）ELISA試劑盒】樣本離心轉(zhuǎn)速及時間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應將其分成小部分-70 ℃保存，避免反復冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應在室溫下解凍并確保樣品均勻地充分解凍。

【大鼠Hepassocin（HPS）ELISA試劑盒】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9240698Cap, Waste Bottle, Modified, APCNY

9243655Addl Unit or Protocol, Comp. Valid SuppEach additional instrument (EZ1, QIAgility, QIAsymphony, QIAcube, Rotor-Gene Q) to be included in the Comprehensive validation service catalog no(s). Scope of work defined from the primary unit utilized in the Comprehensive Validation Support.  Refer to the primary part number for additional information. Quantity of additional units is defined by the number of instruments undergoing a Performance Check study to the primary unit utilized in validation.CNY

1024481#LCX Vacuum SystemHolds 24 QIAamp Maxi columns includes connectors, tubing, waste bottles, vacuum gauge, filters, tray, user manual.CNY

9241221RCS, InstallationRCS Installation facilitates the quick start up of the RCS into the lab routine. It covers hardware ａnd software installation of the system ａnd provides the user with an introductory training on operation ａnd user maintenance. Installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.CNY

330025Microbial DNA qPCR AssaysCNY

9022438USB Ethernet Adaptor, PyroQ96CNY

274202flocktype IBDV Ab (2)CNY

270805cattletype MAP Ab (20)CNY

287015cador M. hyopneumoniae PCR Reagent (96)CNY

285815cador Leptospira PCR Reagent (96)CNY

285205cador B. hyodysenteriae PCR Kit (96)CNY

273001pigtype Salmonella Ab (1)CNY

9020579Jumper, Main Board J5, DML 3000CNY

9020590PCB Assy, Controller, DML 3000CNY

9015908Upg. Kit, Tubing Protection, BR8 (ISI25)CNY

9235642High-speed shaker unit, BR8000CNY

9013227Ferri-Sheld f. Y-flatbandCNY

9013215ZLV Screws, plastic, M3  (20)CNY

9013201Tubing,release val.auto vac,BR8000 v1.00CNY

9011704Cable, PCON98 to AC-box (L1), BR8000CNY

132603NeXtal Stock Propylene glycol (50)100% v/v Propylene glycol - 50mL (4 x 12,5 ml tube)CNY

800302Heterozygote detection, manualCNY

132539NeXtal Stock di-Potassium phosphate (50)3M di-Potassium hydrogen phosphate - 50mL (4 x 12,5 ml tube)CNY

132530NeXtal Stock Cesium sulfate (50)1M Cesium sulfate - 50mL (4 x 12,5 ml tube)CNY

337842qBiomarker SYBR ROX FAST Mastermix (12)CNY

679923ipsogen RT Kit (33) CECNY

180432GeneRead DNA Library I Core Kit (12)CNY

159332Harris UNI-CORE 6.00 mm Punch Kit (4)CNY

159134QIAsafe DNA Blood Tubes (50)CNY

158567Gentra Puregene Yeast/Bact. Kit (200 ml)CNY