1.購買我公司任意ELISA試劑盒指標享受免費代測，并提供技術指導服務！

二、實驗材料與試劑配制：

1. 儀器與材料：酶標儀（使用前預熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時備用（如果標本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【大鼠基質金屬蛋白酶7(MMP-7)ELISA試劑盒】三. 仔細閱讀說明書

1.檢查試劑盒標簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標本制備要規(guī)范，每份標本體積要按2-3個復孔以上的量制備（貯存），盡量分裝做備份。

4.短期內無法實驗者，注意低溫保存

5.準備好所有實驗額外所需物品，（比如移液器，試管，清洗器，酶標儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測標本數(shù)量確定所需試劑的量

7.檢查試劑盒內每種試劑的貯存條件，保證所有試劑均按照試劑盒內說明書推薦的條件存儲。 【大鼠基質金屬蛋白酶7(MMP-7)ELISA試劑盒】

8.檢查不穩(wěn)定或者變質的試劑溶液（比如沉淀或變色）。有些試劑，比如標準品稀釋液或者檢測稀釋液，可能在設計時就含有沉淀物。所有試劑在滴入酶標板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標板之前，必需不停攪拌。

9.保證充分的孵育時間和溫度。

10.切勿使用不同生產批號的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時，避免泡沫產生。

12.在實驗開始之前，安排好實驗流程。在實驗開始之前，清潔工作臺。

13.若有問題，應與我公司或代理商的技術支持聯(lián)系

【大鼠基質金屬蛋白酶7(MMP-7)ELISA試劑盒】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標本盡早檢測，對收集后當天就進行檢測的標本，及時儲存在4℃?zhèn)溆�，如有特殊原因需要周期收集標本，請造模取材后，將標本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質量，所以避免反復凍融。部分標本也可加入適當防腐劑，激素類標本需添加抑肽酶。 

2.試劑盒代測標本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項請與我司技術人員溝通。

3.液體類標本：標本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細胞培養(yǎng)上清、組織勻漿等。

4.購買前請?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測指標及其它特殊要求。

【大鼠基質金屬蛋白酶7(MMP-7)ELISA試劑盒】五、代測數(shù)據(jù)詳細內容

1、ELISA標準曲線；

2、實驗具體詳細步驟；

3、完整的實驗報告（試劑耗材，實驗方法，實驗結果及結果說明）；

4、實驗進行階段，我公司客服人員會及時向您匯報實驗進程。

9235931Splashing guard, BR8 RDCNY

9230822Rack for reagents,Trough (for 6 troughs)CNY

9235707Washer Cable, RCS1CNY

9235695Ground Wire, RCS1CNY

9235332Z-Rack Packing CoverCNY

9235118Fuse F1A, v1.00CNY

9235114Fuse FST4A, v1.00CNY

9230054Clamp, Tubing, Z-barCNY

9234861Lid coupling piece for system liquidCNY

9239551Cbl Assy, XY, LED DriverCNY

9238663Hook, motorized lid lockCNY

9238642Capacitor boardCNY

9238626Opto-EncoderCNY

9238625Eprom ａnd controller 4K15, Zent3CCNY

9241828Pyro Q96 MDa, Premium Agreement"Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"CNY

9241122SuperlubeCNY

9241007QIArent&go - Rent out fee QIAcubeCNY

9240985PCB, drive, cooling device, QsymCNY

9240982Sensor, x, sample tracking, QsymCNY

9240951Support, cable, portal, QsymCNY

9234820Manual, user, QS 4.0, IntCNY

9240768Signal Conditioner, Centrifuge, AP96CNY

9240764DC Output Module 8NPT, 3AMP, AP96CNY

6000-5003Duraseal Sealing FilmCNY

9241781RGQ, Full Agreement, no PM"Repair service delivery: send to Regional Repair Center

Service response time (repair): 7-10 days turnaround time

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: yes, included (provision time 2 days)

Transportation cost coverage: yes, included"CNY

9241776QIAgility, Inspection Service"The Annual Inspection Service, QIAgility is an on-site equipment service, performed by a QIAGEN service specialist. 

All required testing material ａnd certified equipment is provided by the service specialist. 

During the half day-long procedure, all application-critical modules of the QIAgility are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report."CNY

9241756IQ/OQ Services, QIAsymphonyInstrument qualification support service for the QIAsymphony. The product contains the Installation Qualification (IQ)/Operation Qualification (OQ) documentation ａnd the performance of the qualification protocols (travel ａnd labor costs included).CNY

9237126Upgrade App., PCR SetupCNY

9237093Training, Extension dayAdditional days of training may be purchased during the week of the site visit upon request.CNY

9236426Board, arm interconnect, TWICNY