SiR Action cell imaging

The imaging of SiR action is best done using standard Cy5 settings. After labeling, live cells can be immediately imaged without the need for washing steps. Optionally, a simple washing step includes replacing the labeled solution with fresh culture medium without probes, which typically improves the signal-to-noise ratio. If time elapses for imaging, it is recommended to keep the probe concentration equal to or lower than 100 nM throughout the experiment to obtain a constant signal ａnd avoid probe interference with actin dynamics (reducing cell proliferation). If cleaning cells

Before imaging, the staining will last for several hours.

SiR-actin具有熒光性、細(xì)胞通透性和對F-actin的高度特異性。Sir-actin染色內(nèi)源性的F-actin不需要基因操作或過表達(dá)。它在遠(yuǎn)紅光中的發(fā)射大限度地降低了光毒性和樣品的自身熒光。SiR-actin兼容GFP和/或m-cherry熒光蛋白質(zhì)。它可以用標(biāo)準(zhǔn)的Cy5濾鏡進(jìn)行成像。SiR-actin可用于活細(xì)胞和組織的寬視場、共聚焦、SIM或STED成像。探針數(shù)量允許50 - 200染色實(shí)驗(yàn)。*（*基于以下條件:0.5 - 1ml染色液/ 0.5 - 1um探針濃度的染色實(shí)驗(yàn)。染色實(shí)驗(yàn)的數(shù)量可以通過減少體積或探針濃度來進(jìn)一步增加）。

艾美捷 SiR-Actin試劑盒#CY-SC001物理特性：

abs 652 nm

Em 674 nm

ε652 nm 1.0·105 mol-1·cm-1

MW 1241.6 g/mol

MF C71H88N8O10Si

探針濃度（nM） 建議標(biāo)記時(shí)間（h）*

> 1000      0.5 - 1

500           3 - 4

200           4 - 6

< 100        6 - 12

SiR-Actin試劑盒文獻(xiàn)參考：

1. Fluorogenic probes for live-cell imaging of the cytoskeleton, G. Lukinavičius et al., Nature Methods, 11, 731–733 (2014)

來源：https://www.amyjet.com/products/CY-SC001.shtml