英文名字：Horseradish Peroxidase-conjugated AffiniPure Goat Anti-Rabbit IgG （H+L）

編號(hào)：111-035-003

規(guī)格：2ml

應(yīng)用類型：WB（1:10000 - 1:200000）、ELISA（1:5000 - 1:100000） IHC（1:500 - 1:5000）

免疫原：兔IgG

來源宿主：山羊

反應(yīng)性：該山羊抗兔二抗與兔IgG分子有特異性反應(yīng)，與其它兔免疫球蛋白（包括IgM、IgE、IgD、IgA等）的輕鏈有交叉反應(yīng)。該二抗與血清內(nèi)非免疫球蛋白無交叉反應(yīng)，但可能與其它種屬的免疫球蛋白有交叉反應(yīng)。

Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, ａnd freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.

Physical State: Freeze-dried solid

Storage ａnd Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) ａnd centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.

Extended Storage after Rehydration: Aliquot ａnd freeze undiluted product at -20°C or below. Avoid repeated freezing ａnd thawing.

Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.

對(duì)正常血清進(jìn)行脂質(zhì)提取以提高清晰度，用含有疊氮化鈉的磷酸鹽緩沖鹽水（PBS）透析，并冷凍干燥。強(qiáng)烈推薦在PBS中稀釋至5%（v/v）的正常血清作為阻斷試劑，以減少非特異性、保守序列和/或Fc受體結(jié)合的背景。作為添加抗體之的單獨(dú)培養(yǎng)步驟，用來自與標(biāo)記抗體相同宿主的稀釋的正常血清獲得非常好的結(jié)果。

艾美捷HRP標(biāo)記親和純化山羊抗兔IgG（H+L）二抗#111-035-003，基于免疫電泳和/或ELISA，抗體與全分子兔IgG反應(yīng)。它還與其他兔免疫球蛋白的輕鏈發(fā)生反應(yīng)。未檢測到針對(duì)非免疫球蛋白血清蛋白的抗體。抗體可以與來自其他物種的免疫球蛋白發(fā)生交叉反應(yīng)。

通過免疫親和層析從抗血清中分離出完整的IgG抗體分子。它們具有通過二硫鍵連接在一起的Fc部分和兩個(gè)抗原結(jié)合Fab部分，因此它們是二價(jià)的。據(jù)報(bào)道，平均分子量為約160kDa。整個(gè)IgG形式的抗體適用于大多數(shù)免疫檢測程序，并且是具成本效益的。

來源：https://www.amyjet.com/products/111-035-003.shtml