Mouse TNF- αThe ELISA kit uses a double antibody sandwich ELISA method. Using anti mouse TNF- α Antibodies are coated on enzyme labeled plates, ａnd the mouse TNF in the sample or standard during the experiment- α Binding to the coated antibody, the free components are washed away. Add biotinylation anti mouse TNF in turn- α Antibodies ａnd horseradish peroxidase labeled avidins. Anti mouse TNF- α Antibodies ａnd Mouse TNF Binding to Coated Antibodies- α Binding, biotin ａnd avidin specifically bind to form immune complexes, ａnd free components are washed away. By adding a chromogenic substrate (TMB), the TMB appears blue under the catalysis of horseradish peroxidase, ａnd turns yellow after adding a termination solution. Using an enzyme-linked immunosorbent assay (ELISA) to measure the OD value at a wavelength of 450nm, mouse TNF- α The concentration is proportional to the OD450 value, ａnd the mouse TNF in the sample is calculated by plotting a standard curve- α Concentration of.

艾美捷小鼠TNF-α ELISA試劑盒#NDC-KSP-ZWL3G1-96采用定量酶免疫測(cè)定技術(shù)，測(cè)量樣品中的特定抗原。

小鼠TNF-α ELISA試劑盒相關(guān)組成：

小鼠TNF-α ELISA試劑盒基本參數(shù)：

應(yīng)用 酶聯(lián)免疫吸附法

描述 小鼠 TNF-α 酶聯(lián)免疫酶抑制劑試劑盒

類型 檢測(cè)與檢測(cè)

范圍 7.8-500 皮克/毫升

研究域 細(xì)胞信號(hào)傳導(dǎo)

樣品類型 血清，血漿

敏感性 1.0 皮克/毫升

規(guī)格 96t

物種反應(yīng)性 鼠

小鼠TNF-α ELISA試劑盒操作步驟：

1. 在各孔中加入標(biāo)準(zhǔn)品或樣品各100μL, 37℃孵育90分鐘

2. 加入100μL 生物素化抗體工作液,37℃孵育60分鐘

3. 洗滌3次

4. 加入100μL酶結(jié)合物工作液, 37℃孵育30分鐘

5. 洗滌5次

6. 加入90μL底物溶液, 37℃孵育15分鐘左右

7. 加入50μL終止液,立即在450nm波長處測(cè)量OD值

8. 結(jié)果計(jì)算

來源：https://www.amyjet.com/products/NDC-KSP-ZWL3G1-96.shtml