DH2 to be used for 14,15-EET/DHET measurement is the same kit as DH1 for measurement of 14,15-DHET. The only difference with DH2 compared with DH1 is the sample preparation step (NOT ELISA kit) in which EET is chemically changed to DHET.

The 14,15-DHET is a representative metabolite of soluble epoxide hydrolase (sEH)-mediated metabolism of EETs, generated by arachidonic acid epoxygenase activity of cytochromes P450 (CYPs) 2C ａnd 2J. This competitive ELISA kit with an HRP system has been used to determine 14,15-DHET levels in biological samples (tissue,plasma ａnd urine) ａnd cell culture media. Using the 14,15-DHET ELISA, strong positive correlation has been discovered with 14,15-DHET levels on hypertension ａnd brain injury ａnd stroke, ａnd EET levels on a metastatic phenotype of carcinoma cells ａnd insulin resistance. Next generation hypertension drug discoveries have been carried out at several laboratories. Each kit for a 96-well plate is good for triplicate analyses of up to 24 samples.

14，15-DHET是可溶性環(huán)氧化物水解酶（sEH）介導(dǎo)的EET代謝的代表性代謝產(chǎn)物，由細(xì)胞色素P450（CYPs）2C和2J的花生四烯酸環(huán)氧合酶活性產(chǎn)生。這種競(jìng)爭(zhēng)具有HRP系統(tǒng)的ELISA試劑盒已用于測(cè)定生物樣品（組織，血漿和尿液）和細(xì)胞培養(yǎng)基。使用14,15-DHET-ELISA，已發(fā)現(xiàn)強(qiáng)正相關(guān)發(fā)現(xiàn)高血壓、腦損傷和中風(fēng)患者的14,15-DHET水平，轉(zhuǎn)移性腦脊髓炎患者的EET水平癌細(xì)胞表型和胰島素抵抗。下一代高血壓藥物的發(fā)現(xiàn)在幾個(gè)實(shí)驗(yàn)室進(jìn)行。96孔板的每個(gè)試劑盒適用于多24個(gè)樣品的一式三份分析。

（左）自發(fā)性高血壓大鼠尿14,15-DHET水平升高。

（右）妊娠高血壓（先兆子癇）婦女的尿14,15-DHET水平顯著與健康孕婦在98%置信度下的差異（p＜0.02）

艾美捷14,15-DHET Hypertension ELISA#DRD-DH2部分參考文獻(xiàn)：

1. Kim J, Yoon SP, Toews ML, Imig JD, Hwang SH, Hammock BD, Padanilam BJ. Pharmacological inhibition of soluble epoxide hydrolase e prevents renal interstitial fibrogenesis in obstructive nephropathy. Am J Physiol Renal Physiol. 2015 Jan 15;308(2):F131-9. doi: 10.1152/ajprenal.00531.2014. PMID: 25377915.

2. Mattace Raso G, Pirozzi C, d＇Emmanuele di Villa Bianca R, Simeoli R, Santoro, A, Lama A, Di Guida F, Russo R, De Caro C, Sorrentino R, Calignano A, Meli R. Palmitoylethanolamide treatment reduces blood pressure in spontaneously hypertensive rats: involvement of cytochrome p450-derived eicosanoids ａnd Renin Angiotensin system. PLoS One. 2015 May 7;10(5):e0123602. doi: 3.1371/journal.pone.0123602. eCollection 2015. PMID: 25951330.

10. Dai M, Wu L, He Z, Zhang S, Chen C, Xu X, Wang P, Gruzdev A, Zeldin DC, Wang DW. Epoxyeicosatrienoic Acids Regulate Macrophage Polarization ａnd Prevent LPS-Induced Cardiac Dysfunction. J Cell Physiol. 230(9):2108-19. 2015 doi: 10.1002/jcp.24939. PMID: 25626689.

14,15-DHET Hypertension ELISA相關(guān)研究：

14,15-DHET Immunoassay Kit     DH1

BioTarget 14,15-DHET Human Urine ELISA Kit DH3

14,15-DHET Immunoaffinity Column for One Step Purification     DH31

14,15-DHET sEH activity ELISA Kit    SH1

來(lái)源：https://www.amyjet.com/products/BXC-BE0045-1mg.shtml