樣品稀釋液：ICT-959樣品稀釋優(yōu)化包注意事項：

為了快速確定哪種樣品稀釋劑配方適合測定，所有三種樣品稀釋劑都可以在同一IgG或抗原包被的ELISA板上平行運行。

確定在三種不同的樣品稀釋劑類型中制備的ELISA曲線的初始動態(tài)分析物范圍。

在血清樣本中加入測試尖峰。

讀取三條SD相關標準曲線中的每一條上的加標血清樣品孔，以查看哪種樣品稀釋劑提供了添加到血清樣品中的加標濃度的佳近似值。

在檢測到高水平基質復雜性干擾的情況下，可能還需要加入額外的測定稀釋劑試劑，以使校準孔抗體與抗原結合動力學更接近樣品孔結合動力學。

ICT樣本稀釋液：ICT-647一般血清稀釋劑引文：

To quickly determine which Sample Diluent formulation is best for the assay, all three Sample Diluents can be run in parallel on the same IgG or antigen-coated ELISA plate.

Determine the initial dynamic analyte range of the ELISA curves that were prepared in each of the three different Sample Diluent types.

Make test spikes into serum samples.

Read the spiked serum sample wells on each of the three SD-associated standard curves to see which Sample Diluent gives the best approximation of the spike concentration that was added to the serum samples.

In cases where a high level of matrix complexity interference is detected, inclusion of an additional Assay Diluent reagent may also be required to bring the calibrator well antibody-to-antigen binding kinetics closer to the sample well binding kinetics.

來源：https://www.amyjet.com/products/ICT-647.shtml