艾美捷谷胱甘肽檢測(cè)試劑盒利用精確地酶促循環(huán)的方法，通過(guò)谷胱甘肽還原酶來(lái)定量樣本中的GSH.GSH的巰基基團(tuán)可以與DTNB反應(yīng)產(chǎn)生黃色TNB，混合的二流產(chǎn)物GSTNB能被谷胱甘肽還原酶還原成GSH，并形成更多TNB.TNB的產(chǎn)出率與樣本中GSH的含量成比例.TNB的吸收光為405或者412nm.GSH能很容易被氧化成二硫聚合物GSSH.

艾美捷谷胱甘肽檢測(cè)試劑盒基本參數(shù)：

中文名稱：谷胱甘肽分析試劑盒-96次分析

英文名字：Glutathione Assay Kit

編號(hào)：703002-96

規(guī)格：96wells

保存建議：4°C下保存.

艾美捷谷胱甘肽檢測(cè)試劑盒相關(guān)文獻(xiàn)參考：

1. Baker, M.A., Cerniglia, G.J., ａnd Zaman, A. Microtiter plate assay for the measurement of glutathione ａnd glutathione disulfide in large numbers of biological samples. Anal. Biochem. 190, 360-365 (1990).

2. Eyer, P., ａnd Podhradsky, D. Evaluation of the micromethod for determination of glutathione using enzymatic cycling ａnd Ellman’s reagent. Anal. Biochem. 153, 57-66 (1986).

3. Tietze, F. Enzymic method for quantitative determination of nanogram amounts of total ａnd oxidized glutathione: Applications to mammalian blood ａnd other tissues. Anal. Biochem. 27, 502-522 (1969).

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