FAM FLICA Caspase-1探針允許研究人員評(píng)估Caspase-1的激活。FLICA試劑FAM-YVAD-FMK進(jìn)入每個(gè)細(xì)胞，并不可逆地與活化的caspase-1結(jié)合。因?yàn)?span style="font-family: Calibri;">FAM-YVAD-FMK FLICA試劑與活性酶共價(jià)偶聯(lián)，所以它保留在細(xì)胞內(nèi)，而任何未結(jié)合的FAM-YVAD-FMK-FLICA試劑擴(kuò)散出細(xì)胞并被沖走。剩余的綠色熒光信號(hào)是添加試劑時(shí)細(xì)胞中存在的活性caspase-1酶活性的直接測(cè)量。含有結(jié)合的FLICA的細(xì)胞可通過(guò)熒光平板讀取器、熒光顯微鏡或流式細(xì)胞儀進(jìn)行分析。用FLICA試劑標(biāo)記的細(xì)胞可立即讀取或使用試劑盒中的固定劑保存16小時(shí)。還可以用碘化丙啶或Hoechst 33342分析未固定的樣品，以分別檢測(cè)核形態(tài)的壞死或變化。

艾美捷ICT FLICA天冬氨酸蛋白酶Caspase-1活性檢測(cè)試劑盒參數(shù)：

試劑名稱FAM-YVAD-FMK

目標(biāo)Caspase-1

激發(fā)/發(fā)射488nm/530 nm

分析方法流式細(xì)胞儀、熒光顯微鏡、熒光板讀取器

樣品類型細(xì)胞培養(yǎng)

儲(chǔ)存2-8°C

艾美捷ICT FLICA天冬氨酸蛋白酶（Caspase）活性檢測(cè)試劑盒組分：

Kit 97: 25 Tests

FLICA Caspase-1 Reagent (FAM-YVAD-FMK), 1 vial, #653

10X Apoptosis Wash Buffer, 15 mL, #635

Fixative, 6 mL, #636

Propidium Iodide, 1 mL, #638

Hoechst 33342, 1 mL, #639

Kit Manual

Kit 98: 100 Tests

FLICA Caspase-1 Reagent (FAM-YVAD-FMK), 4 vials, #653

10X Apoptosis Wash Buffer, 60 mL, #634

Fixative, 6 mL, #636

Propidium Iodide, 1 mL, #638

Hoechst 33342, 1 mL, #639

Kit Manual

天冬氨酸蛋白酶（Caspase）活性檢測(cè)試劑盒部分文獻(xiàn)參考：

Hu, R;Liang, J;Ding, L;Zhang, W;Liu, X;Song, B;Xu, Y. Edaravone dexborneol provides neuroprotective benefits by suppressing NLRP3 inflammasome-induced microglial pyroptosis in experimental ischemic stroke. International immunopharmacology. 2022 October 21; doi: 10.1016/j.intimp.2022.109315. Article

Yuan, X;Bhat, OM;Zou, Y;Li, X;Zhang, Y;Li, PL. Endothelial Acid Sphingomyelinase Promotes NLRP3 Inflammasome ａnd Neointima Formation during Hypercholesterolemia. Journal of lipid research. 2022 October 14; doi: 10.1016/j.jlr.2022.100298. Full Text

Wan, Y;Zhang, W;Huang, C;Jian, J;Zhang, Y;Liu, Q;Chen, P;Zhu, X. Ursolic acid alleviates Kupffer cells pyroptosis in liver fibrosis by the NOX2/NLRP3 inflammasome signaling pathway. International immunopharmacology. 2022 October 14; doi: 10.1016/j.intimp.2022.109321. Text

來(lái)源：https://www.amyjet.com/products/ICT-97.shtml