程序：雞用C-Myc免疫與KLH綴合的肽。多次之后用弗氏佐劑免疫收集。使用固定在固體上的肽對(duì)抗體進(jìn)行免疫親和純化階段

規(guī)范：

ELISA：抗體特異性通過(guò)針對(duì)與BSA綴合的肽的ELISA。A.1： 38000稀釋的抗體得到OD=

1.0，在15分鐘反應(yīng)中使用HRP-綴合的山羊抗雞IgY（IgG）1： 10000和TMB作為基底。適當(dāng)?shù)倪M(jìn)行特異性對(duì)照。

宿主：雞

格式：液體PBS中的親和純化抗體，pH 7.2含0.1%疊-氮-化鈉。

Kamiya艾美捷抗-C-Myc，多克隆儲(chǔ)存和穩(wěn)定性：

在+4°C下儲(chǔ)存。

應(yīng)用和建議的稀釋液：酶聯(lián)免疫吸附試驗(yàn)

蛋白質(zhì)印跡：約1:5000時(shí)使用特定應(yīng)用的佳稀釋度應(yīng)由研究人員確定。

僅用于體外研究。不適用于診斷或人類。

Kamiya艾美捷抗-C-Myc，多克隆英文說(shuō)明：

Procedure:

Chickens were immunized with the C-Myc

peptide conjugated with KLH. After multiple

immunizations in Freund’s adjuvant, eggs were

collected. Antibodies were immunoaffinitypurified using the peptide immobilized on a solid

phase.

Specification:

ELISA: Antibody specificity was verified by

ELISA against the peptide conjugated to BSA. A

1:38,000 dilution of the antibody gave an OD =

1.0 in a 15 minute reaction using HRPconjugated Goat anti-Chicken IgY (IgG) at

1:10,000 ａnd TMB as the substrate. Appropriate

specificity controls were run.

Host: Chicken

Format:

Liquid. Affinity-purified antibody in PBS, pH 7.2

with 0.1% sodium azide.

Storage ａnd Stability:

Store at +4°C.

Applications ａnd Suggested Dilutions:

ELISA

Western blot: Use at ~1:5,000

The optimal dilution for a specific application

should be determined by the researcher.

Limitations:

For in vitro research use only. Not for use in

diagnostics or in humans.

Kamiya基于免疫比濁技術(shù)的臨床診斷和研究免疫分析工具，用于脂質(zhì)評(píng)估、凝血、炎癥、糖尿病、營(yíng)養(yǎng)評(píng)估和血清蛋白測(cè)量。艾美捷科技是Kamiya的中國(guó)代理商，為科研工作者提供優(yōu)質(zhì)的產(chǎn)品與服務(wù)。

來(lái)源：https://www.amyjet.com/brand/Kamiya.shtml