備選名稱：CCKi-8

應(yīng)用：比色檢測(cè)

數(shù)量：100次測(cè)試：1 ml

500次測(cè)試：5 ml

2500次測(cè)試：5 x 5 mL

處理：避光。避免冷凍/解凍循環(huán)。

運(yùn)輸：藍(lán)冰運(yùn)輸

長(zhǎng)期儲(chǔ)存：-20°C

內(nèi)容：WST-8溶液，1-甲氧基-PMS

艾美捷細(xì)胞計(jì)數(shù)試劑盒-8（CCK-8）原理：

使用四氮唑鹽WST-8（2-（2-甲氧基-4-硝基苯基）-3-（4-硝基苯基）-5-（2,4-二磺基苯基）-2H-四氮唑單鈉鹽），其在電子載體1-甲氧基-PMS存在下生化還原時(shí)產(chǎn)生高度水溶性的甲氮染料。細(xì)胞中脫氫酶產(chǎn)生的黃色甲贊染料的量與培養(yǎng)基中活細(xì)胞的數(shù)量成正比。

細(xì)胞計(jì)數(shù)試劑盒-8（CCK-8）是一種比色測(cè)定試劑盒，用于測(cè)量細(xì)胞增殖和細(xì)胞毒性。它是一種不需要放射性同位素且與[3H]-胸苷摻入試驗(yàn)相關(guān)的即用溶液。它可以直接添加到細(xì)胞培養(yǎng)基中，用于快速、高通量篩選，而無(wú)需增溶過(guò)程，從而獲得高度重復(fù)和準(zhǔn)確的結(jié)果。CCK-8顯示出比MTT、MTS或WST-1更高的靈敏度和穩(wěn)定性。

圖2：CCK-8對(duì)HeLa和HL60細(xì)胞的敏感性比MTT更敏感 

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4-Phenylbutyric acid, a potent endoplasmic reticulum stress inhibitor, attenuates the severity of collagen-induced arthritis in mice via inhibition of proliferation ａnd inflammatory responses of synovial fibroblasts: Y. Choi, et al.; Kaohsiung J. Med. Sci. 37, 604 (2021), Application(s): Rheumatoid arthritis synovialfibroblasts (RASFSs) proliferation assay, Abstract;

Bioactive small molecules in calcium phosphate scaffold enhanced osteogenic differentiation of human induced pluripotent stem cells: B. Song, et al.; Dent. Mater. J. 40, 615 (2021), Abstract;

Cargo proteins in extracellular vesicles: potential for novel therapeutics in non-alcoholic steatohepatitis: J. Kim, et al.; J. Nanobiotechnology 19, 372 (2021), Abstract;

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