艾美捷Jackson 正常兔血清產(chǎn)品可應(yīng)用于大多數(shù)免疫學(xué)實(shí)驗(yàn),用作封閉液或空白對照.

艾美捷Jackson 正常兔血清使用：

正常兔血清是以凍干粉形式提供的，本品需要先溶解配制成100%原液后再稀釋使用。溶解方法：用5 mL 蒸餾水（dH2O）充分溶解凍干粉（如果不澄清，可對其離心處理），此時(shí)得到的即是100%原液。然后按照1:20的比例用緩沖液，如PBS或PBST，稀釋到需要的5%工作液。也可以根據(jù)自身實(shí)驗(yàn)條件，稀釋到合適的工作液濃度。

艾美捷011-000-120正常兔血清化學(xué)性質(zhì)：

全名：正常兔血清丨Normal Rabbit Serum

Cat#：011-000-120

規(guī)格：5ml

蛋白濃度（Protein concentration）：60 mg/mL

緩沖液（Buffer）：0.01 M磷酸鈉，0.25 M氯化鈉，pH 7.6

防腐劑（Preservative）：0.05%die氮化鈉

運(yùn)輸與保存方法：冰袋運(yùn)輸。凍干粉保存于2-8℃。

Information

Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, ａnd freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.

Usage

Physical State: Freeze-dried solid

Storage ａnd Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O ａnd centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.

Extended Storage after Rehydration: Aliquot ａnd freeze undiluted product at -20°C or below. Avoid repeated freezing ａnd thawing.

Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.

來源：https://www.amyjet.com/products/011-000-120.shtml