在藥物研發(fā)過程中，對總膽汁酸TBA的準(zhǔn)確監(jiān)控必不可少，那么，如何檢測小鼠總膽汁酸呢？

老款試劑盒80470已被淘汰，建議使用zui新款的80471，更少的樣本需求，更穩(wěn)定的試劑保存，更穩(wěn)定的實(shí)驗(yàn)結(jié)果。艾美捷 Crystal Chem高分文章引用的升款小鼠總膽汁酸檢測試劑盒，能很好的解決這問題。該試劑盒檢測原理如下：

小鼠總膽汁酸檢測試劑盒是利用3-a羥基類固醇脫氫酶(3-aHSD)特有的酶學(xué)性質(zhì)，以酶學(xué)技術(shù)為基礎(chǔ)開發(fā)的。在NAD存在下，膽汁酸轉(zhuǎn)化為3-酮類固醇和NADH。生成的NADH與硝基四唑藍(lán)(NBT)反應(yīng)形成染料。通過在540nm處測量吸光度來監(jiān)測染料的形成，吸光度與小鼠樣品中的膽汁酸濃度成正比。

Nature引用的CCM-80471超敏小鼠總膽汁酸檢測試劑盒，You, Sangmin, et al. "Dysregulation of bile acids increases the risk for preterm birth in pregnant women." Nature communications 11.1 (2020): 1-15.

其它發(fā)表文章（僅摘取部分展示）：

1、Cao, Lijuan, et al. "Repression of intestinal transporters ａnd FXR-FGF15 signaling explains bile acids dysregulation in experimental colitis-associated colon cancer."Oncotarget38 (2017): 63665.

2、Martinot, E., Baptissart, M., Véga, A. et al. Bile acid homeostasis controls CAR signaling pathways in mouse testis through FXRalpha. Sci Rep 7, 42182 (2017).

3、Lai, Qiuwen, et al. "E2F1 inhibits circulating cholesterol clearance by regulating Pcsk9 expression in the liver." JCI insight 2.10 (2017).

4、Zhang, Jun, et al. "Chronic over-expression of fibroblast growth factor 21 increases bile acid biosynthesis by opposing FGF15/19 action." EBioMedicine 15 (2017): 173-183.

5、Boué, Stephen, et al. "A novel gastrointestinal microbiome modulator from soy pods reduces absorption of dietary fat in mice." Obesity 24.1 (2016): 87-95.

6、Chin, Siew Hung, et al. "Opposing effects of reduced kidney mass on liver ａnd skeletal muscle insulin sensitivity in obese mice." Diabetes 64.4 (2015): 1131-1141.

來源：https://www.amyjet.com/featured/tba.shtml