【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【人檸檬酸水解酶(ACL)試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人檸檬酸水解酶(ACL)試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人檸檬酸水解酶(ACL)試劑盒現(xiàn)貨】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人檸檬酸水解酶(ACL)試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問題請(qǐng)來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

51183      QIAamp DNA Blood Midi Kit (20) For 20 DNA midipreps: 20 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml)       CNY                           

9015518  Cable bare, Y         CNY                           

9001885  QIAcube (230V) System       QIAcube (230V) System combines the QIAcube system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                           

9001873  EZ1 Advanced, PrioPLUS      "EZ1 Advanced Instrument

Installation of EZ1 instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"   CNY                           

9001869  Rotor-Gene Q 6plex (PC) Priority Rotor-Gene Q 6plex (PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service ａnd response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents. CNY                           

9018449  Piston, locate (1), QX           CNY                           

9015450  Upgrade kit, support sheet, DISU v2.1         CNY                           

9015448  Pump, dispenser, BR8000, v2.0           CNY                           

9015447  Modules, DISU, BR8 RD v2.0             CNY                           

9015409  Belt, Y-timing, v2, M96         CNY                           

291053    mericon GMO Screen CTP2-CP4EPSPS (24)            CNY                            【人檸檬酸水解酶(ACL)試劑盒現(xiàn)貨】

9015387  Maintenance Kit, BR MDx            CNY                           

9001850  QIAsymphony RGQ System         CNY                           

9012291  Reagent holder tray        CNY                           

133077    NeXtalStock PEG 2,000 (200)             CNY                           

133076    NeXtalStock PEG 10,000 (200)           CNY                           

9015380  Unit, heat/cool, left, M96              CNY                           

9015373  Magnet, M96         CNY                           

9015371  Plungers, M96 (8)         CNY                           

133154    NeXtalStock BIS-TRIS Propane pH6.5 (100)             CNY                           

133153    NeXtalStock BIS-TRIS pH 8.5 (100)           CNY                           

204545    QuantiTect Multiplex PCR Kit (1000)    "1x 25 ml Master Mix

1x 20 ml RNase free water"   CNY                           

133062    NeXtalStock Magnesium nitrate (200)         CNY                           

133061    NeXtalStock Magnesium formate (200)              CNY                           

870011    therascreen KRAS RGQ PCR Kit CE (24)           CNY                           

290055    mericon VTEC stx1/2 Kit (96)             CNY                           

204341    QuantiTect Probe PCR Kit (40)     "-Trial kit size: 40 x 50 µl rxns at an economical price

- 1x 1ml 2x Master Mix, 1x 2 ml RNase free water

For quantitative, real-time PCR ａnd two-step RT-PCR using sequence-specific probes."   CNY                            【人檸檬酸水解酶(ACL)試劑盒現(xiàn)貨】

9018392  Key, Data Review, grey, QIAxcel FA           CNY                           

9012271  Power Supply, 24V / 8Amp, (9194)             CNY                           

9001647  Rotor-Gene Q 5plex (w/o PC) System  Rotor-Gene Q 5plex (w/o PC) System includes the Rotor-Gene instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the Rotorgene-Q systems hardware ａnd trains the user on operation of the Rotorgene software. All travel ａnd labor cost included for Installation ａnd service documented on a GMP/GLP-compliant Field Service document.     CNY                           

9015332  Sensor, pressure, M48          CNY                           

 QY-03060       抗堿性磷酸酶抗體       Anti-AP/ALP(Alkaline phosphatase)                                

 QY-03061       α－甲基酰基輔酶A消旋酶抗體      Anti-AMACR/P504S(alpha-methylacyl-CoA racemase)                                 

 QY-03062       神經(jīng)元突觸前膜蛋白抗體    Anti-Amph(Amphiphysin)                                

 QY-03063       腺苷單磷酸活化蛋白激酶α1抗體     Anti-AMPK alpha-1 (AMP-activated Protein Kinase-α1)                              

 QY-03064       腺苷單磷酸活化蛋白激酶β1抗體     Anti-AMPKβ1 (AMP-activated Protein Kinase beta-1)                                 

 QY-03065       糖尿病相關(guān)肽/胰島淀粉樣肽抗體      Anti-Amylin                              

 QY-03066       β淀粉樣肽1-42 (C端抗體)       Anti-β-Amyloid 1-42（Aβ1-42）(CT)                                

 QY-03067       β淀粉樣肽（1-16）抗體    Anti-β-Amyloid（1-16）                               

 QY-03068       β淀粉樣肽（31-35）抗體  Anti-β-Amyloid（31-35）                              

 QY-03069      β淀粉樣肽（1-28）抗體   Anti-β-Amyloid（1-28）                               

 QY-03070       小鼠抗β淀粉樣肽（1-28）抗體        Anti-β-Amyloid（1-28）                               

 QY-03071       β淀粉樣肽（25-35）抗體  Anti-β-Amyloid（25-35）                              

 QY-03072       β淀粉樣肽（1-40）抗體    Anti-β-Amyloid（1-40)                                  

 QY-03073       β淀粉樣肽β-Amyloid 1-40/Aβ1-40 C端抗體        Anti-β-Amyloid 1-40/Aβ1-40（CT）                                   【人檸檬酸水解酶(ACL)試劑盒現(xiàn)貨】

 QY-03074       β淀粉樣肽 1-40 C端抗體  Anti-β-Amyloid 1-40（CT）mouse                               

 QY-03075       β淀粉樣肽（1-42）抗體    Anti-β-Amyloid（1-42）                               

 QY-03076       血管生成素-1抗體       Anti-ANG-1/(Angiopoietin 1; angiopoietin-like 4)                            

 QY-03077       血管生成素-2抗體       Anti-ANG-2 (Angiopoietin 2)hu, mo, rat, pig, dog, chi                                  

 QY-03078       錨定蛋白G抗體   Anti-Ankyrin G/ANK-3/ankyrin 3                            

 QY-03079       心鈉肽（心鈉素）抗體       Anti-ANP(atrial natriuretic peptide)                                 

 QY-03080       血管緊張素Ⅱ-2型受體抗體       Anti-AT2R（Angiotensin Ⅱ Type 2 Receptor）                                  

 QY-03081       血管緊張素II抗體       Anti-AT-Ⅱ (Angiotensin Ⅱ)                                  

 QY-03082       血管位蛋白樣2/血管生成素樣蛋白-2抗體       Anti-ANGPTL2/ANG-2 (Angiopoietin-like Protein 2)                             

 QY-03083       血管位蛋白樣4/血管生成素樣蛋白-4抗體       Anti-ANGPTL4/ANG-4 (Angiopoietin-like Protein 4)                             

 QY-03084       膜粘連蛋白A1抗體     Anti-ANXA1/P35(Annexin A1)                                

 QY-03085       膜粘連蛋白 I抗體       Anti-Annexin I                                  

 QY-03086       膜粘連蛋白 Ⅱ抗體     Anti-Annexin Ⅱ                               

 QY-03087       膜粘連蛋白 Ⅲ抗體     Anti-Annexin Ⅲ                               

 QY-03088       膜粘連蛋白 Ⅳ抗體     Anti-Annexin Ⅳ                               

 QY-03089       膜粘連蛋白 V抗體      Anti-Annexin V                                  【人檸檬酸水解酶(ACL)試劑盒現(xiàn)貨】

 QY-03090       膜粘連蛋白 VI抗體     Anti-Annexin VI                               

 QY-03091       重組膜粘連蛋白-5(人)抗體  Anti-Annexin V (Recomb Humen Annexin V)                                 

 QY-03092       炭疽菌抗體    Anti-Anthrax