價(jià)格詳細(xì)資料：試劑盒價(jià)格電議，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認(rèn)同。齊一生物大品牌保證，傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)產(chǎn)品。

【人1-3-P-D葡聚糖（G)elisa試劑盒廠家】注意事項(xiàng)

1. 當(dāng)混合蛋白溶液時(shí)應(yīng)盡量輕緩，避免起泡。

2. 洗滌過(guò)程非常重要，不充分的洗滌易造成假陽(yáng)性。

3. 一次加樣時(shí)間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。

4. 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線，做復(fù)孔。

5. 如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高，請(qǐng)先稀釋后再測(cè)定，計(jì)算時(shí)請(qǐng)*后乘以稀釋倍數(shù)。

6. 在配制標(biāo)準(zhǔn)品、檢測(cè)溶液工作液時(shí)，請(qǐng)以相應(yīng)的稀釋液配制，不能混淆。

7. 底物請(qǐng)避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人1-3-P-D葡聚糖（G)elisa試劑盒廠家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人1-3-P-D葡聚糖（G)elisa試劑盒廠家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人1-3-P-D葡聚糖（G)elisa試劑盒廠家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

4863004       pg NDV Univ. RT-PCR ASSAY LE        CNY                          

4873004       pg AIV-H5 RT-PCR ASSAY           CNY                          

130923  NeXtal DWBlock Classics II Suite          CNY                          

130920  NeXtal DWBlock JCSG+ Suite        CNY                          

4621063       artus MTHFR LC PCR Kit (24) CE        CNY                          

4841014       pg HPV PCR ASSAY        CNY                          

4555265       artus M.tuberculosis RG PCR Kit (96) CE             CNY                          

4551065       artus Borrelia LC PCR Kit (96) CE         CNY                          

4513303       artus HI Virus-1 QS-RGQ Kit (24) RUO              CNY                          

4510033       artus Enterovirus LC RT-PCR Kit (24) PRE          CNY                           【人1-3-P-D葡聚糖（G)elisa試劑盒廠家】

130718  NeXtal Tubes PACT Suite         CNY                          

122001  SeqTarget Primer Select (400)          CNY                          

4506165       artus HBV TM PCR Kit (96) CE            CNY                          

120016  QIAquick 96 Plates (24)    Individual QIAquick 96 plates ａnd handbook. No buffers or reagents.       CNY                          

75162    RNeasy Maxi Kit (12) 12 RNeasy Maxi Spin Columns, Collection Tubes (50 ml), RNase-free Reagents ａnd Buffers   CNY                          

74704    RNeasy Fibrous Tissue Mini Kit (50)       50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), Proteinase K, RNase-free DNase I, RNase-free Reagents ａnd Buffers      CNY                          

27406    QuickLyse Miniprep Kit (250)          CNY                          

73504    RNeasy FFPE Kit (50)              CNY                          

73442    RNeasy Plus Universal Tissue Midi Kit10        CNY                          

72041    Oligotex Direct mRNA Midi/Maxi Kit      For 6 mRNA midi or 2 maxipreps: 1 ml Oligotex Suspension, Large Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents ａnd Buffers      CNY                          

26173    R.E.A.L. Prep 96 Plasmid Kit (24)   For 24 x 96 rapid extraction alkaline lysis minipreps: 24 QIAfilter 96 Plates, Square-Well Blocks, Tape Pads, Reagents, Buffers       CNY                          

20051    QIAEX II Gel Extraction Kit (500)   For 500 extractions: 5 x 1.0 ml QIAEX II Suspension, Buffers    CNY                          

19743    QIAfilter Midi Cartridges (25)   25 QIAfilter Midi Cartridges      CNY                          

69982    TissueLyser Adapter Set (2 x 24)      2 sets of Adapter Plates ａnd 2 racks for use with 2.0 ml microcentrifuge tubes on the TissueLyser. Also contains 6 silicone sealing strips. (3 per adapter) to ensure 2-ml tubes are secure.  CNY                          

19413    QIAvac 24 Plus           CNY                          

19088    Reagent DX         CNY                           【人1-3-P-D葡聚糖（G)elisa試劑盒廠家】

19086    Buffer EB (250ml)       250 ml Elution Buffer   CNY                          

19073    Buffer AVL    "Buffer AVL (5x 31 ml, 250 preps)

(""QIAamp Viral Lysis Buffer, includes 5x 310 µg Carrier RNA (26.6 ug/ml), 270 Mini preps"")"   CNY                          

19051    Buffer P1       500 ml Resuspension Buffer (RNase A not included)     CNY                          

16181    QIAGEN Plasmid Plus 96 Miniprep  Kit (4)        CNY                          

12462    QIAGEN Large-Construct Kit (10)  10 QIAGEN-tip 500, Reagents, Buffers, ATP-Dependent Exonuclease       CNY                          

QY-16562    Anti-NT-3神經(jīng)營(yíng)養(yǎng)因子-3抗體                                    

QY-16572    Anti-NT-4/NT-5（Neurotrophic factor 4/5）神經(jīng)生長(zhǎng)因子4/5抗體                                     

QY-16582    Anti-NTN（Neurturin ）神經(jīng)生長(zhǎng)因子抗體                                       

QY-16592    Anti-Nurr1核受體相關(guān)因子-1抗體                                       

QY-16602    Anti-OAS1（2＇,5＇-oligoadenylate synthetase 1）寡腺苷酸合成酶-1                                        

QY-16612    Anti-OAT-1 (Organic anion transporter 1)human陰離子轉(zhuǎn)運(yùn)蛋白-1(抗人;RC-0607R 抗大、小鼠)                                       

QY-16622    Anti-OAT-1 (Organic anion transporter 1)mouse、rat陰離子轉(zhuǎn)運(yùn)蛋白-1（抗大、小鼠：RC-0606R 抗人）                                        

QY-16632    Anti-OAT-3(Organic anion transporter 3)陰離子轉(zhuǎn)運(yùn)蛋白-3抗體                                    

QY-16642    Anti-OAT4L/URAT1 (Urate Transporter 1)尿酸鹽重吸收轉(zhuǎn)運(yùn)子1抗體                                       

QY-16652    Anti-OCT1 (organic cat-ion transporter1)陽(yáng)離子轉(zhuǎn)運(yùn)器1抗體                                        

QY-16662    Anti-OCT2 (organic cat-ion transporter2)B細(xì)胞特異性轉(zhuǎn)錄因子抗體                                   

QY-16672    Anti-Oct-4 antibody胚胎干細(xì)胞關(guān)鍵蛋白抗體                                   

QY-16682    anti-ODC（Ornithine decarboxylase）抗鳥(niǎo)氨酸脫羧酶抗體                                    

QY-16692    Anti-Omgp (olyigodendrocyte myelin glycoprotein)少突細(xì)胞髓磷脂糖蛋白抗體                                   

QY-16702    Anti-OPCML (opioid binding protein/cell adhesion molecule-like)阿片樣物質(zhì)結(jié)合蛋白/細(xì)胞粘附分子樣蛋白抗體                                        【人1-3-P-D葡聚糖（G)elisa試劑盒廠家】

QY-16712    Anti-OPG (Osteoprotegerin)骨保護(hù)蛋白/護(hù)骨素抗體                                        

QY-16722    Anti-OPGL/RANKL (Osteoprotegerin ligand)骨保護(hù)蛋白配體抗體                                        

QY-16732    Anti-OPN(osteopontin)骨橋蛋白抗體                                     

QY-16742    Anti-OPN(osteopontin)骨橋蛋白抗體                                     

QY-16752    Anti-ORX-A （orexin-A）增食欲素-A/欲激素A                                      

QY-16762    Anti-Osx (Osterix)成骨相關(guān)轉(zhuǎn)錄因子抗體                                    

QY-16772    anti-Os05g/0477200 protein抗水稻白葉枯病菌Os05g0477200蛋白抗體                                         

QY-16782    anti-OTR(oxytocin receptor）縮宮素受體抗體                                     

QY-16792    Anti-OVA雞卵白蛋白/卵清蛋白抗體                                   

QY-16802    Anti-OXR (Orexin receptor)食欲素受體抗體                                        

QY-16812    Anti-P14ARF抑癌基因p14抗體                                    

QY-16822    Anti-P16/CDKN2A (Cyclin-dependent kinase inhibitor-2A)抑癌基因p16抗體                                     

QY-16832    Anti-P19ARFp19ARF抑癌基因抗體                                     

QY-16842    Anti-P21/WAF1/CIP1p21抗體                                         

QY-16852    Anti-P27/kip1 (cyclin-dependent kinase inhibitor 1B)P27抗體/周期素依賴激酶抑制劑                                      

QY-16862    Anti-P2Y1 receptorP2Y1受體抗體                                          【人1-3-P-D葡聚糖（G)elisa試劑盒廠家】

QY-16872    Anti-P311 protein神經(jīng)再生相關(guān)蛋白抗體                                    

QY-16882    Anti-P38 MAPK(MAPK14 non-phospha)絲裂原活化蛋白激酶p38抗體