檢測(cè)標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測(cè)方法：ELISA

檢測(cè)類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠(chéng)為您服務(wù)！本試劑盒用于測(cè)定血清,血漿及相關(guān)液體樣本中含量.

【人S100鈣結(jié)合蛋白A12/鈣粒蛋白C(S100A12)Elisa廠家】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值）,通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人S100鈣結(jié)合蛋白A12/鈣粒蛋白C(S100A12)Elisa廠家】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過(guò)氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人S100鈣結(jié)合蛋白A12/鈣粒蛋白C(S100A12)Elisa廠家】

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測(cè)樣品孔.在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測(cè)定：以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）. 測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開(kāi)封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果. 【人S100鈣結(jié)合蛋白A12/鈣粒蛋白C(S100A12)Elisa廠家】

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說(shuō)明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說(shuō)明書有異,以英文說(shuō)明書為準(zhǔn).

計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見(jiàn)應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

9018925  Adapter, 33x1.5ml, Tapered-Base Tubes                   CNY                             

9018906  36-Well Rotor Locking Ring               CNY                             

9018562  Board, power branching, EZ1a                  CNY                             

9013102  BGR Arm P7x82 Redesign (7186)              CNY                             

672591     ipsogen RARA-PML bcr1&2 Standards              CNY                             

672391     ipsogen PML-RARA bcr3 Standards          CNY                             

670513     ipsogen BCR-ABL1 Mbcr (GUS) Kit (24)             CNY                             

619024     careBrush, CE           CNY                              【人S100鈣結(jié)合蛋白A12/鈣粒蛋白C(S100A12)Elisa廠家】

389002     Investigator IDproof Single Key                 CNY                             

9001515  PyroMark Q24 MDx Vacuum Workstation       For preparation of single stranded DNA template ready for sequencing by PyroMark Q24.(220V) CE-marked.  CNY                             

9001512  HC2 Equipment Package (non-US)            CNY                             

9001340  AUTOPURE LS           CNY                             

4868004  pg FMDV RT-PCR ASSAY            CNY                             

4872004  PG AIV-H5 RT-PCR Kit (48)  Z          CNY                             

4846014  careUU PCR ASSAY            CNY                             

4559065  artus C.trachomatisPlus LC PCR Kit(96)CE                 CNY                             

4551063  artus Borrelia LC PCR Kit (24) CE               CNY                             

130717     NeXtal Tubes ComPAS Suite               CNY                             

130715     NeXtal Tubes ProComplex Suite                 CNY                             

4506263  artus HBV RG PCR Kit (24) CE           CNY                             

120015     QIAprep Turbo Buffer Module 8000         "6 x 250 ml each: Buffers P1, P2, N3; 12 x 100 ml Buffer PE (concentrate); 3 x 250 ml Buffer EB; 6 x 1.65 ml RNase A (25 mg/ml solution); handbook

Buffers to run QIAprep Turbo 96 on a BioRobot 8000 (24 x 96-well plates)"  CNY                             

79654       QIAshredder (50)     50 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps       CNY                             

76526       RNAprotect Cell Reagent (250 ml)           CNY                             

74192       Rneasy Plus 96 Kit (12)              CNY                              【人S100鈣結(jié)合蛋白A12/鈣粒蛋白C(S100A12)Elisa廠家】

27405       QuickLyse Miniprep Kit (100)            CNY                             

27173       QIAprep 96 Turbo Core Kit (24)                 CNY                             

20902       QIAEX II Suspension 1.5 ml suspension     CNY                             

133269     NeXtalStock Phosphate-citr. pH 4.2 (200)                 CNY                             

990522     Filter-Tips, 200 µl (960)            CNY                             

990396     QIAcube Modified Protocol               CNY                             

4501263  artus EBV RG PCR Kit (24) CE            CNY                             

QY-15902 Anti-MrpL28 (mitochondrial ribosomal protein L28)線粒體核糖體蛋白L28抗體                                                       【人S100鈣結(jié)合蛋白A12/鈣粒蛋白C(S100A12)Elisa廠家】

QY-15912 Anti-MSH-2 (MutS homolog 2)錯(cuò)配修復(fù)蛋白2抗體                                                   

QY-15922 anti-MLH1(Mutl homolog l gene)錯(cuò)配修復(fù)蛋白1抗體                                                       

QY-15932 Anti-MSLN (mesothelin)間皮素抗體                                                     

QY-15942 anti-MUC5AC/Mucin 5AC(Gastric Mucin M1)胃粘液素抗體                                               

QY-15952 Anti-MTR-1A (Melatonin receptor-1A)褪黑素受體/松果體素受體抗體                                                    

QY-15962 Anti-mucin-1/Muc-1/CD227 antigen (Epithelial Membrane Antigen )粘蛋白-1/上皮膜抗原抗體                                              

QY-15972 Anti-MyD88 (myeloid differential protein-88)髓樣分化蛋白抗體                                              

QY-15982 Anti-Myelin P0 protein( peripheral myelin prothein Zero;MPZ;MPP)外周髓磷脂P0蛋白/P0蛋白抗體                                                    

QY-15992 Anti-Myosin (Smooth Muscle)鼠抗人心肌肌凝蛋白(平滑肌) 單抗                                                   

QY-16002 Anti-N-AChR α4 (Nicotinic-Acetylcholine receptor α4)煙堿型乙酰膽堿受體α4抗體                                                 

QY-16012 Anti-N-AChR α7 (Nicotinic-Acetylcholine receptor α7)煙堿型乙酰膽堿受體α7抗體                                                 

QY-16022 Anti-Nanog胚胎干細(xì)胞關(guān)鍵蛋白抗體                                               

QY-16032 anti-Natrexone抗納曲酮抗體IgG                                                       

QY-16042 Anti-NAP1 (nucleosome assembly protein 1)核小體組裝蛋白1抗體                                                 

QY-16052 Anti-N-cadherinN-鈣粘附分子抗體                                                      

QY-16062 Anti-N-coR1 （Nuclear receptor co-repressor 1）核受體輔助抑制因子抗體                                                   【人S100鈣結(jié)合蛋白A12/鈣粒蛋白C(S100A12)Elisa廠家】

QY-16072 Anti-Nephrin Protein腎病蛋白抗體                                                     

QY-16082 Anti-Nestin巢蛋白/神經(jīng)上皮干細(xì)胞蛋白抗體                                                  

QY-16092 Anti-Nestin巢蛋白/神經(jīng)上皮干細(xì)胞蛋白抗體                                                  

QY-16102 Anti-Neurobeachin protein (AKAP550)蛋白激酶錨定蛋白/激酶固定蛋白抗體                                                

QY-16112 Anti-Neurocan神經(jīng)粘蛋白抗體                                                  

QY-16122 Anti-Neurofascin-155神經(jīng)束蛋白-155                                                

QY-16132 Anti-NF-H（Neurofilament triplet H)高分子量神經(jīng)絲蛋白抗體                                               

QY-16142 Anti-NFKBp65(p65 NF-kappa B;p65NFKB)細(xì)胞核因子/k基因結(jié)合核因子抗體                                                 

QY-16152 Anti-NF-L（Neurofilament triplet L)低分子量神經(jīng)絲蛋白抗體                                                 

QY-16162 Anti-NF-M (Neurofilament triplet M）中分子量神經(jīng)絲蛋白抗體                                                      

QY-16172 Anti-NF-κBp50（p50 NF-kappa B;p50NFKB）細(xì)胞核因子50/κ基因結(jié)合核因子50抗體                                                    

QY-16182 Anti-NGF-R/p75NTR/CD271（p75 Neurotrophin R）神經(jīng)生長(zhǎng)因子受體抗體                                                  

QY-16192 Anti-NGF-β神經(jīng)生長(zhǎng)因子-β抗體                                                      

QY-16202 anti-NGN3（neurogenin 3; Neurog3）神經(jīng)元素3抗體                                                     

QY-16212 Anti-NGX6 (nasopharyngeal carcinoma/NPC associated gene 6)鼻咽癌細(xì)胞相關(guān)基因6抗體                                                  

QY-16222 Anti-NHE1（Na+/H+ Exchanger）鈉氫通道蛋白抗體                                                【人S100鈣結(jié)合蛋白A12/鈣粒蛋白C(S100A12)Elisa廠家】