1. 酶標(biāo)儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人金黃色葡萄球菌腸毒素C（SEC）試劑盒現(xiàn)貨】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！

ELISA應(yīng)用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗的各領(lǐng)域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【人金黃色葡萄球菌腸毒素C（SEC）試劑盒現(xiàn)貨】齊一生物科技?xì)g迎您訂購咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實(shí)驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實(shí)驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【人金黃色葡萄球菌腸毒素C（SEC）試劑盒現(xiàn)貨】齊一生物供應(yīng)的ELISA試劑盒ELISA實(shí)驗原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測.先將生物素標(biāo)記的抗體同時溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標(biāo)準(zhǔn)品,對ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽性. 【人金黃色葡萄球菌腸毒素C（SEC）試劑盒現(xiàn)貨】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費(fèi)代測）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測試劑盒、比色法檢測試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

6000-5022        Multi-SpecimenTube(MST)Vortexer II, 240V            CNY                             

9240688  1/4-28 Nylock nut, AP                 CNY                             

330033     Microbial DNA qPCR Assay Kits                 CNY                             

1053394  Buffer RW1 (220 ml)                  CNY                             

275003     flocktype NDV Ab (5)                 CNY                             

274503                       CNY                             

274303     flocktype IBV Ab (5)          CNY                              【人金黃色葡萄球菌腸毒素C（SEC）試劑盒現(xiàn)貨】

282307     virotype PRRSV RT-PCR Kit (480)               CNY                             

9241818  Pyro Q24, Basic Agreement     """Repair service delivery: send to Regional Repair Center

Service response time (repair): 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"""       CNY                             

929527     #QX Alignment Marker 15bp/400bp (1.5 ml)            CNY                             

9020581  Y-Motor, DML3000           CNY                             

9022473  Foam Inserts, Shipment, DML3000          CNY                             

9013396  Fitting, soft wall (10)                 CNY                             

9013393  Tubing set, wash pump, 200             CNY                             

9013266  Cover, screw, cool/heat. (10)            CNY                             

9013192  Tubing, base outlet, auto vac, BR8000             CNY                             

9011756  Heat Transfer Adapter, Microplate RB    Adapter for accommodating a 96-well microplate RB on the cooling ａnd heating system or the cooling system; used for both cooling ａnd heating   CNY                             

9011708  Cable, LSB to AC, BR8000                  CNY                             

132738     NeXtal Stock Dioxane (200)     100% v/v Dioxane - 200mL (20 x 10 ml tube)  CNY                             

132600     NeXtal Stock Potassium sodium tar. (50) 1.5M Potassium sodium tartrate tetrahydrate - 50mL (4 x 12,5 ml tube)       CNY                             

800343     Cosmid End-Sequencing, w/o prep           CNY                             

133294     NeXtalStock Zinc chloride 200)                  CNY                             

133288     NeXtalStock TRIS-HCl pH 8.4 (200)           CNY                              【人金黃色葡萄球菌腸毒素C（SEC）試劑盒現(xiàn)貨】

1013335  Ni-NTA HisSorb 384 Plates, black (100)             CNY                             

997008     Reagent Cartridge Holder (2)           CNY                             

997004     8-Rod Covers (144)            CNY                             

994900     Filter-Tips ａnd Holders, EZ1    50 Disposable Filter-Tips, 50 Disposable Tip Holders; additional tips ａnd holders for use with BioRobot EZ1 Kits    CNY                             

674891     ipsogen TCF3-PBX1 Standards          CNY                             

674391     ipsogen Beta-Globine Control Gene Stds                   CNY                             

337830     qBiomarker SYBR Fluor Mastermix (2)              CNY                             

337633     qBiomarker SYBR Green ROX Mastermix (24)                   CNY                             

QY-15572 Anti-M-CSF (Macrophage Colony Stimulating Factors)巨噬細(xì)胞克隆刺激因子抗體                                                  

QY-15582 Anti-MDM2 (urine double minute 2)雙微體2癌基因抗體                                                  【人金黃色葡萄球菌腸毒素C（SEC）試劑盒現(xiàn)貨】

QY-15592 Anti-Megsin/SER—PINB7絲氨酸(或半胱氨酸)蛋白酶抑制劑B7抗體                                                     

QY-15602 Anti-Melan-A/MART-1黑色素瘤相關(guān)抗原/黑色素-A抗體                                                

QY-15612 Anti-Metal ion transporter擬南介金屬離子轉(zhuǎn)運(yùn)蛋白抗體                                              

QY-15622 Anti-Mfn1 (Mitofusin1)線粒體融合蛋白1抗體                                                  

QY-15632 Anti-MGMT (O6-methylguanine-DNA methyltransferase)O6甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶抗體                                                     

QY-15642 anti-MT(metallothionein)金屬基質(zhì)硫蛋白抗體                                                  

QY-15652 anti-MGr1-Ag/37LRP(P37-kDa laminin receptor precursor)（NT）層粘連蛋白受體1抗體（N端）                                                       

QY-15662 anti-MGr1-Ag/37LRP(P37-kDa laminin receptor precursor)（CT）層粘連蛋白受體1抗體（C端）                                              

QY-15672 Anti-MICA(MHC class I polypeptide-related sequence A)一種細(xì)胞應(yīng)激分子抗體                                                      

QY-15682 Anti-Midnolin isoform Protein 1中腦核仁蛋白1抗體                                               

QY-15692 Anti-Midnolin isoform Protein 2中腦核仁蛋白2抗體                                               

QY-15702 Anti-MIF (Macrophage Migration Inhibitory Factor)巨噬細(xì)胞移動抑制因子抗體                                                     

QY-15712 Anti-MIP-1α (macrophage inflammatory protein 1α)巨噬細(xì)胞炎癥因子1α抗體                                               

QY-15722 Anti-MIP-1β (macrophage inflammatory protein 1β)巨噬細(xì)胞炎癥因子1β 抗體                                                      

QY-15732 Anti-MMP-1(matrix metalloproteinases-1)基質(zhì)金屬蛋白酶-1抗體                                                   

QY-15742 Anti-MMP-1(matrix metalloproteinases-1)anti-Mouse基質(zhì)金屬蛋白酶-1抗體（小鼠）                                                【人金黃色葡萄球菌腸毒素C（SEC）試劑盒現(xiàn)貨】

QY-15752 Anti-MMP-13 (Matrix metalloproteinase 13)基質(zhì)金屬蛋白酶13抗體                                                      

QY-15762 Anti-MMP-14(Matrix metalloproteinase-14)基質(zhì)金屬蛋白酶-14抗體                                                       

QY-15772 Anti-MMP-2(Collagenase IV /Gelatinase A/Metallo proteinase-2)基質(zhì)金屬蛋白酶-2抗體                                               

QY-15782 Anti-MMP-3(matrix metalloproteinase-3/Transin-1/SL-1/Stromelysin-1 precursor)基質(zhì)金屬蛋白酶-3抗體                                                       

QY-15792 Anti-MMP-7(Matrilysin/matrix metalloproteinases-7)基質(zhì)金屬蛋白酶-7抗體                                                 

QY-15802 Anti-MMP-9(matrix metalloproteinase 9)基質(zhì)金屬蛋白酶-9抗體                                                     

QY-15812 Anti-β-2-MG鼠抗人β2微球蛋白抗體(單抗)                                                  

QY-15822 Anti-Mo anti-KLH小鼠抗血藍(lán)蛋白抗體                                                      

QY-15832 Anti-MOG (myelin oligo-dendrocyte glycoprotein-MOG)髓鞘少樹突膠質(zhì)細(xì)胞糖蛋白抗體                                                      

QY-15842 Anti-Mouse anti-human HAS鼠抗人血清白蛋白單克隆抗體                                                    

QY-15852 Anti-Mouse IgA兔抗小鼠IgA抗體                                                      

QY-15862 Anti-MPO (myeloperoxidase)髓過氧化物酶抗體                                                

QY-15872 Anti-MRP1(Multidrug Resistanec-Associated Protein 1)多藥耐藥相關(guān)蛋白1抗體                                                   

QY-15882 Anti-MRP2 (multidrug resistance-associated protein2)多藥耐藥相關(guān)蛋白2抗體                                                     

QY-15892 Anti-MRP3(Multidrug Resistanec-Associated Protein 3)多藥耐藥相關(guān)蛋白3抗體