1. 酶標(biāo)儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購買說明！【人8異前列腺素α（8-ISO-PGα）elisa試劑盒現(xiàn)貨】

ELISA應(yīng)用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【人8異前列腺素α（8-ISO-PGα）elisa試劑盒現(xiàn)貨】齊一生物銷售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人8異前列腺素α（8-ISO-PGα）elisa試劑盒現(xiàn)貨】ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測.先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽性.

以上資料僅供參考了解，不供于論文寫作

【人8異前列腺素α（8-ISO-PGα）elisa試劑盒現(xiàn)貨】齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費(fèi)代測）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測試劑盒、比色法檢測試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

5000-1430   MST Vortexer II - FoamFeetAssembly, 110V        CNY            

5128-1220   Specimen Transport Medium (STM) CE      CNY            

5123-1220   Female Swab Specimen Collection KitTM       CNY            

5100-1400IVD    hc2 Sample Conversion Kit       CNY            

EA11424 EASYartus BKV RG_II     CNY            

5050-1075 V.1   CD Rom Manual, MST Vortexer 2       CNY            

EA10124 EASYartus EBV RG PCR Kit 2      CNY            

BR-02   DxS B-RAF Mutation Test Kit     CNY            

9240729 Tubing, 3/8"OD , Red, AP        CNY            

9240719 Cable Carrier, Capper, Elevator, AP     CNY            

9240701 O-Ring, Shaker, Lower Body, AP96/196        CNY             【人8異前列腺素α（8-ISO-PGα）elisa試劑盒現(xiàn)貨】

9240696 Fitting, Barbed, 1/4" ID tube, AP       CNY            

9243651 Comprehen Valid., QIAgility """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   For planning purposes ａnd to ensure a smooth process, the Customer is expected to have all materials on-hand ａnd inventoried one (1) week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """ CNY            

218076  miScript SYBR Green PCR Kit (2000)      CNY            

271906  cattletype Milk Prep Kit (50)       CNY            

289980  intype IC-DNA (1ml)     CNY            

280437  virotype BTV RT-PCR Kit (480)       CNY            

932836  #QIAsymphony Ni-NTA Denat. Kit (384)        CNY            

800511  Verification Sequencing / DNA production        CNY            

9238243 ZLV BioRobot M96 User Manual        CNY            

9019988 ZLV Mainboard, QGY      CNY            

9020553 Foam angle, transport box, QIAC     CNY            

9020582 X-Motor, DML3000        CNY            

9020584 Y-Belt, DML3000 320 Teeth       CNY            

9020594 Enclosure, DML3000      CNY            

9021006 Motor, Pipettor Y, DCU      CNY            

9236333 Sample Loop     CNY             【人8異前列腺素α（8-ISO-PGα）elisa試劑盒現(xiàn)貨】

9013382 Shaker adapter, microplate, LHS     CNY            

9013275 Motor, gripper, handler T/L, BR3000     CNY            

9013269 Fitting, PA tubing M6x1, 2.25mm, (10)       CNY            

9013210 Cabling kit, moving, BR8000     CNY            

QY-11612    anti-Clostridium perfringens type D兔抗D型產(chǎn)氣莢膜梭菌抗體                    

QY-11622    Anti-C-Mer/MERTK (proto-oncogene tyrosine kinase)c-mer原癌基因酪氨酸激酶抗體                       

QY-11632    Anti-C-Met/HGFR（Hepatocyte growth factor receptor）肝細(xì)胞生長因子受體(原癌基因蛋白)抗體                       

QY-11642    Anti-C-Myc/MYC/MTLC致癌基因抗體                    

QY-11652    anti-c-Myc tagc-Myc tag標(biāo)簽抗體                    

QY-11662    Anti-CNP/CNPase ( 2＇,3＇-cyclic-nucleotide 3＇-phosphodiesterase)C型鈉尿肽抗體                       

QY-11672    anti-CNTF（Ciliary Neurotrophic Factor）睫狀神經(jīng)營養(yǎng)因子抗體                       

QY-11682    Anti-Collagen Ⅱ(Anti-Collagen Type Ⅱ)抗Ⅱ型膠原抗體(Collagen Ⅱ Ⅱ型膠原)                    

QY-11692    Anti-Collagen Ⅲ(Anti-Collagen Type Ⅲ)抗Ⅲ型膠原抗體(Collagen Ⅲ Ⅲ型膠原)                    

QY-11702    Anti-Collagen Ⅳ(Anti-Collagen Type Ⅳ)抗IV型膠原抗體(Collagen Ⅳ Ⅳ 型膠原)                      

QY-11712    Anti-Collagen Ⅵ（Collagen Type Ⅵ)抗Ⅵ型膠原抗體                      

QY-11722    Anti-Collagen I(Anti-Collagen Type I)抗I型膠原抗體(I型膠原)                       

QY-11732    Anti-CollagenⅤ(Anti-Collagen Type Ⅴ)抗Ⅴ型膠原抗體(CollagenⅤ Ⅴ型膠原)                      

QY-11742    Anti-CollagenⅩ(Collagen TypeⅩ)抗Ⅹ型膠原抗體                      【人8異前列腺素α（8-ISO-PGα）elisa試劑盒現(xiàn)貨】

QY-11752    Anti-COX-1 (Prostaglandin-endoperoxide synthase-1)前列腺素氧化環(huán)化酶1抗體                     

QY-11762    Anti-COX-2 (Prostaglandin-endoperoxide synthase-2)前列腺素氧化環(huán)化酶2抗體                     

QY-11772    Anti-C-PC(c-phycocyanin )C-藻藍(lán)素/藻藍(lán)蛋白抗體                     

QY-11782    Anti-C-Peptide（ C-PEP ）C-肽                      

QY-11792    Anti-CR (Calretinin)鈣結(jié)合蛋白抗體                     

QY-11802    Anti-C-reactive protin CRPC-反應(yīng)蛋白抗體                       

QY-11812    Anti-C-CRP（Mouse Anti-Human reactive protin CRP Antibody）鼠抗人C-反應(yīng)蛋白單克隆抗體                      

QY-11822    Anti-CREB-1(cAMP responsive element binding protein-1)環(huán)腺苷酸應(yīng)答元件結(jié)合蛋白抗體                     

QY-11832    Anti-CREB-1(cAMP responsive element binding protein-1)環(huán)腺苷酸應(yīng)答元件結(jié)合蛋白抗體                     

QY-11842    Anti-P-CREB-1(p-cAMP responsive element binding protein-1)磷酸化環(huán)腺苷酸應(yīng)答元件結(jié)合蛋白抗體                      

QY-11852    Anti-CRF (Corticotropin-Releasing Factor)促腎上腺皮質(zhì)激素釋放因子                      

QY-11862    Anti-CRF (Corticotropin-Releasing Factor)促腎上腺皮質(zhì)激素釋放因子                      

QY-11872    anti-CSP (circumsporozoite protein/Plasmodium yoelii)環(huán)子孢子蛋白(約氏瘧原蟲)抗體                      

QY-11882    Anti-CT （Calcitonin )降鈣素抗體                       

QY-11892    Anti-CT DNA (culf thymus DNA)兔抗小牛胸腺DNA 抗體                      

QY-11902    Anti-CTGF (connective tissue growth factor)結(jié)締組織生長因子抗體                     【人8異前列腺素α（8-ISO-PGα）elisa試劑盒現(xiàn)貨】

QY-11912    Anti-CTLA-4/CD152（cytotoxic T-lymphocyte associated antigen-4）淋巴細(xì)胞相關(guān)抗原4/細(xì)胞毒性T細(xì)胞抗原-4抗體                       

QY-11922    anti-CTn1 (cardiac troponin 1)心肌肌鈣蛋白抗體                     

QY-11932    Anti-CT-R（Calcitonin receptor）降鈣素受體抗體