【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【人恙蟲病抗體IgM(STA-IgM)ELISA試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人恙蟲病抗體IgM(STA-IgM)ELISA試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人恙蟲病抗體IgM(STA-IgM)ELISA試劑盒現(xiàn)貨】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人恙蟲病抗體IgM(STA-IgM)ELISA試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問題請(qǐng)來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

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9016530  Transportation box 1, LiquiChip           CNY                           

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9016325  Washer head with holder , P 13xx        CNY                           

9019185  PCB - Front, PyroQ96MD            CNY                           

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9011824  Z-Modul; Tip-Adapter 1100µl; BR8000        CNY                           

9237033  Cable, Liquid Sensor, BR8 RD v1.0             CNY                           

9236960  Probe Holder, Body, BR8 RD        CNY                            【人恙蟲病抗體IgM(STA-IgM)ELISA試劑盒現(xiàn)貨】

9019144  Fan - rear 92x92, PyroQ96MD            CNY                           

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9019075  PyroMark NDT Holder, PyroQ96MD   Re-usable holder for tips, Nucleotide Dispensing Tips ａnd Reagent Dispensing Tips, for use in PyroMark Q96 MD.      CNY                           

9019010  Motor M, EZ1xl            CNY                           

9018953  Reagent Block, 16x0.2ml/2ml PCR/Flat-Bas        CNY                           

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9018935  Adapter, reagent tube SBS Plate           CNY                           

9015837  Upg. Kit, Y6L Connection, BR8 (ISI21)              CNY                           

9015689  Fittings (10), M6/3.3 mm, white          CNY                           

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9015523  O-rings, plunger (12)            CNY                           

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9019167  Linear actuator, PyroQ96MD        CNY                           

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QY-10952       Anti-CD20CD20抗體                                      

QY-10962       Anti-CD24CD24抗體                                      

QY-10972       Anti-CD25/IL-2RA(Imterleukm-2 Receptor alpha)CD25/白介素2-受體抗體                                      

QY-10982       Anti-CD272/BTLA(B ａnd T lymphocyte attenuator)B和T淋巴細(xì)胞衰減蛋白抗體                                   

QY-10992       Anti-CD2AP (CD2-associated protein)白細(xì)胞分化抗原CD2AP抗體                                         

QY-11002       Anti-CD3CD3抗體                                         

QY-11012       Anti-CD3（Mouse Anti-Human CD3 Monoclonal Antibody）鼠抗人CD3單克隆抗體                                      

QY-11022       Anti-CD31（PECAM-1）血小板內(nèi)皮細(xì)胞黏附分子-1抗體                                       

QY-11032       Anti-CD31（PECAM-1）血小板內(nèi)皮細(xì)胞黏附分子-1抗體                                       

QY-11042       Anti-CD34CD34抗體（細(xì)胞表面的唾液粘蛋白）                                      

QY-11052       Anti-CD36CD36抗體                                      

QY-11062       Anti-CD4（Mouse Anti-Human CD4 Monoclonal）鼠抗人CD4單克隆抗體                                     

QY-11072       Anti-CD4（Rabbit Anti-Human Mouse rat CD4 Palyclonal Antibody）CD4抗體                                        

QY-11082       Anti-CD4（Rabbit Anti-Human Mouse rat CD4 Palyclonal Antibody）CD4抗體                                        

QY-11092       anti-CD40L (CD40 Ligand/TNFSF5/CD154)抗CD40L抗體                                        

QY-11102       Anti-CD44CD44抗體                                       【人恙蟲病抗體IgM(STA-IgM)ELISA試劑盒現(xiàn)貨】

QY-11112 Anti-CD45(LCA)CD45(白細(xì)胞共同抗原)抗體                                      

QY-11122       Anti-CD49/VLA(very late antigens)CD49(非常晚期抗原)抗體                                     

QY-11132       Anti-CD5CD5抗體                                         

QY-11142       Anti-CD54/ICAM-1(Intercellular dhesion molecule 1/CD54 antigen;)細(xì)胞間粘附分子-1抗體                                   

QY-11152       Anti-ICAM-2/CD102（intercellular adhesion molecules-2）細(xì)胞間粘附分子-2抗體                                         

QY-11162       Anti-CD56/NCAM1(Neural Cell Adhesion Molecule 1)神經(jīng)細(xì)胞粘附分子1抗體（Elisa IP IHC 用）                                     

QY-11172       Anti-CD56/NCAM1(Neural Cell Adhesion Molecule 1)神經(jīng)細(xì)胞粘附分子1抗體                                       

QY-11182       Anti-CD62L (ELAM-1/L-Selectin )L選擇素抗體                                   

QY-11192       anti-E-Selectin E選擇素抗體                                         

QY-11202       Anti-CD68（Rabbit Anti-Mouse rat CD68 Palyclonal Antibody）CD68抗體（用于大鼠、小鼠）                                   

QY-11212       Anti-CD73(5´-nucleotidase cytosolicⅡ)胞漿-5´-核苷酸酶-Ⅱ抗體                                    

QY-11222       Anti-CD79A/IGBP-1(Immunoglobuin binding protein-1)免疫球蛋白結(jié)合蛋白-1抗體                                       

QY-11232       Anti-CD8（Mouse Anti-Human CD8 Monoclonal Antibody）鼠抗人CD8單克隆抗體                                      

QY-11242       Anti-CD8（Rabbit Anti-Human Mouse rat CD8 Palyclonal Antibody）CD8抗體                                        

QY-11252       Anti-CD86/B7-2CD86抗體                                     

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QY-11272       Anti-CDK2 (Cyclin-Dependent Kinase 2)周期素依賴性激酶2抗體