檢測標本：血清,血漿,尿液,胸腹水,腦脊液,細胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價格及詳細資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認同.大品牌保證,價格公道,傾力為國內外科研院校實驗室提供*優(yōu)質的產(chǎn)品.若有需要,我司將竭誠為您服務！本試劑盒用于測定血清,血漿及相關液體樣本中含量.

【人流感嗜血桿菌（HI）IGMELISA廠家】實驗原理：

   本試劑盒應用雙抗體夾心法測定標本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標記的本產(chǎn)品抗體結合,形成抗體-抗原-酶標抗體復合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉化成藍色,并在酸的作用下轉化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關.用酶標儀在450nm波長下測定吸光度（OD值）,通過標準曲線計算樣品中該產(chǎn)品濃度.

試劑盒組成：

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人流感嗜血桿菌（HI）IGMELISA廠家】標本要求：

1．標本采集后盡早進行提取,提取按相關文獻進行,提取后應盡快進行實驗.若不能馬上進行試驗,可將標本放于-20℃保存,但應避免反復凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標準品的稀釋與加樣：在酶標包被板上設標準品孔10孔,在、第二孔中分別加標準品100μl,然后在、第二孔中加標準品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標準品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標準品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標準品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標準品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人流感嗜血桿菌（HI）IGMELISA廠家】

2.     加樣：分別設空白孔（空白對照孔不加樣品及酶標試劑,其余各步操作相同）、待測樣品孔.在酶標包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標板孔底部,盡量不觸及孔壁,輕輕晃動混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復5次,拍干.

6.     加酶：每孔加入酶標試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應（此時藍色立轉黃色）.

11. 測定：以空白空調零,450nm波長依序測量各孔的吸光度（OD值）. 測定應在加終止液后15分鐘以內進行.

注意事項：

1． 試劑盒從冷藏環(huán)境中取出應在室溫平衡15-30分鐘后方可使用,酶標包被板開封后如未用完,板條應裝入密封袋中保存.

2． 濃洗滌液可能會有結晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結果. 【人流感嗜血桿菌（HI）IGMELISA廠家】

3． 各步加樣均應使用加樣器,并經(jīng)常校對其準確性,以避免試驗誤差.一次加樣時間控制在5分鐘內,如標本數(shù)量多,推薦使用排搶加樣.

4． 請每次測定的同時做標準曲線,做復孔.如標本中待測物質含量過高（樣本OD值大于標準品孔孔的OD值）,請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計算時請*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請避光保存.

7． 嚴格按照說明書的操作進行,試驗結果判定必須以酶標儀讀數(shù)為準.

8． 所有樣品,洗滌液和各種廢棄物都應按傳染物處理.

9． 本試劑不同批號組分不得混用.

10. 如與英文說明書有異,以英文說明書為準.

計算：

以標準物的濃度為橫坐標,OD值為縱坐標,   

在坐標紙上繪出標準曲線,根據(jù)樣品的OD     

值由標準曲線查出相應的濃度；再乘以稀釋     

倍數(shù)；或用標準物的濃度與OD值計算出標     

準曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實際濃度.

試劑盒性能：

1.樣品線性回歸與預期濃度相關系數(shù)R值為0.95以上.

2.批內與批見應分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個月

9014794  Board, YZ6L, slave, BR8000              CNY                             

9014772  Card, X-main, bar code              CNY                             

9014686  Cable, Arm/East, BR8, RD                 CNY                             

9014535  RCS, Arm Cable, Flat, RCS1                CNY                             

9014497  Wash Station, complete, BR8 RD             CNY                             

9022475  Foam Inserts, Shipment, careHPV            CNY                              【人流感嗜血桿菌（HI）IGMELISA廠家】

9022273  PyroMark Q24 Plate Holder, PyroQ24               CNY                             

9021624  Kit, 8 ch pip. plunger upgrade, Cx8          CNY                             

9230198  Sealing ring, 2.5/1.5 mm           CNY                             

9238671  Cap, white                 CNY                             

9238651  Interference suppression filter F11.126/           CNY                             

9238641  Fan, power board ａnd casing  this is a 5.25" fan that is mounted horizontally underneath the centrifuge.  CNY                            

9238637  Diode BYW 35           CNY                             

9240970  Motor, x, sample tracking, Qsym              CNY                             

9240965  X-Ring, pipetting system, Qsym                 CNY                             

9240683  Spring, Elevator Pickup Head, AP196/98           CNY                             

9240814  #Agreement, EZ1 DSP, PM, 1YR                CNY                             

9240788  Update, Pump Pulley/Motor, AP98          CNY                             

6000-5008        RCS Reagent Troughs                CNY                             

6000-5004        Sealing Film Dispenser              CNY                             

9241768  QIAxtractor, Basic Agreement         The Basic Cover Agreement, QIAxtractor provides full service coverage for the QIAxtractor ａnd the CAS 1280. Costs for service visits are fully covered including travel, labor, ａnd parts. One on-site pipetting head service ａnd inspection is included. With a Basic Cover Agreement, there is a response time of 5 working days.   CNY                             

9241656  Transfer Rack Plate, AP             CNY                              【人流感嗜血桿菌（HI）IGMELISA廠家】

9241630  Shaker Adjustment Kit, AP                CNY                             

9241367  Damper, door, EZ1a                   CNY                             

9240759  Update, Sliding Door Latch, AP98             CNY                             

9243548  Pyro Q96 ID, PM Service "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9242766  Cogwheel, x-drive, portal, Qsym               CNY                             

9242710  Validation Support, 2d, QIAgility      The QIAGEN QIAgility Guided Validation Support service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the QIAgility system ａnd software, review the User Manual, ａnd practical hands-on operation. The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which processes (quant setup, normalization, PCR setup, CE setup) ａnd kit manufacturer (Applied Biosystems, Promega, QIAGEN) need to be included in the validation.     CNY                             

9242253  Cable, x, portal AS, Qsym                   CNY                             

9242234  RGQ, IQ/OQ Service         "Instrument qualification support service for the Rotorgene Q ａnd Rotorgene 6000. The product contains the IQ/OQ documentation ａnd the performance of the qualification protocols (travel ａnd labor costs included).

During the service, the compliance with the manufacturer＇s installation requirements is testified ａnd the performance verification & dynamic calibration of the Rotorgene Q is performed." CNY                             

9242172  Repair fee, Depot Repair, TissueLyser II           CNY                             

QT-00180 陰溝腸桿菌ATCC13047 凍干粉                                         

QT-00181 表皮葡萄球菌CMCC(B)29069        凍干粉                                         

QT-00182 阪崎腸桿菌ATCC29544 凍干粉                                          【人流感嗜血桿菌（HI）IGMELISA廠家】

QT-00183 弗氏檸檬酸桿菌ATCC43864 凍干粉                                         

QT-00184 肺炎克雷伯氏菌ATCC10031 凍干粉                                         

QT-00185 小腸結腸炎耶爾森氏菌ATCC23715      凍干粉                                         

QT-00186 亞利桑那沙門氏菌CMCC(B)47001        凍干粉                                         

QT-00187 熱帶念珠菌ATCC13803 凍干粉                                         

QT-00188 克柔假絲酵母菌ATCC14243 凍干粉                                         

QT-00189 光滑念珠菌ATCC15126 凍干粉                                         

QT-00190 創(chuàng)傷弧菌ATCC27562      凍干粉                                         

QT-00191 嬰兒雙歧桿菌ATCC15697      凍干粉                                         

QT-00192 德式乳桿菌保加利亞亞種CICC6032     凍干粉                                         

QT-00193 生孢梭菌CMCC（B）64941  凍干粉                                         

QT-00194 嗜熱鏈球菌CICC6038     凍干粉                                         

QT-00195 SphingobacteriumcompostiDSM18850   凍干粉                                         

QT-00196 豬霍亂沙門氏菌豬霍亂亞種DSM4224         凍干粉                                         

QT-00197 沼澤紅假單胞菌DSM5859    凍干粉                                         

QT-00198 嗜鹽細菌屬DSMZ16465         凍干粉                                          【人流感嗜血桿菌（HI）IGMELISA廠家】

QT-00199 土壤伯克氏菌DSMZ18235    凍干粉                                         

QT-00200 煙酸芽孢桿菌DSMZ2923      凍干粉                                         

QT-00201 菲律賓鏈霉菌DSMZ40112=ATCC23905         凍干粉                                         

QT-00202 抗輻射鏈霉菌DSMZ41901    凍干粉                                         

QT-00203 短乳桿菌DSMZ6235      凍干粉                                         

QT-00204 乳酸片球菌JCM8791=DSM20284=NCIMB12174  凍干粉                                         

QT-00205 桃色歐文氏菌NBRC102418  凍干粉                                         

QT-00206 白色噬瓊膠菌NBRC102603  凍干粉                                         

QT-00207 浮游球衣菌NBRC13543         凍干粉                                         

QT-00208 丁香假單胞菌NBRC14078     凍干粉                                         

QT-00209 睪丸酮叢毛單胞菌NBRC14951     凍干粉                                         

QT-00210 耐放射異常球菌NBRC15346         凍干粉                                         

QT-00211 煙酸芽孢桿菌NBRC15566     凍干粉                                         

QT-00212 新鞘氨醇單胞菌NBRC16725         凍干粉