【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【人水通道蛋白4（AQP-4-AB）抗體elisa現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人水通道蛋白4（AQP-4-AB）抗體elisa現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人水通道蛋白4（AQP-4-AB）抗體elisa現(xiàn)貨】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人水通道蛋白4（AQP-4-AB）抗體elisa現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問題請(qǐng)來(lái)電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9240780  500 ml Reagent #6 Update, AP196        CNY                           

9240772  Assy, Sub, Dispense Head, AP96          CNY                           

9240769  Mono Touch Screen, AP96           CNY                           

9241465  IQ/OQ Services, QIAcube     Instrument qualification support service for the QIAcube. The product contains the IQ/OQ documentation ａnd the performance of the qualification protocols (travel ａnd labor costs included).       CNY                           

9243647  Acceler. Valid Supp, QuantiPlex/QP Hyres    The QIAGEN Quantiplex/Quantiplex HYres Accelerated Validation Support service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introductory seminar about the Quantiplex/Quantiplex HYres quantification chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on user reaction setup, ａnd best practices.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through Quantiplex/Quantiplex HYres validation, as well as general wet lab support such as pipetting for quantification to produce the data needed for the validation study. The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.  CNY                           

9243646  Guided Valid Support, Other Quant. Sys       The QIAGEN Guided Validation Support, Other Quantification System service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide hands-on user reaction setup, ａnd best practices.  The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to validate (Plexor HY, Quantifiler Human, Quantifiler Y, Quantifiler Duo)  CNY                           

9243541  #Rapid Plate, PM Service      "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                            【人水通道蛋白4（AQP-4-AB）抗體elisa現(xiàn)貨】

9243115  Lee Sample Valve, LC           CNY                           

9242863  QIArent&go - Rent out fee QSAS Cabinet           CNY                           

9242771  Tool, align encoder, Qsym            CNY                           

9242717  Validation Support, 5d, QIAcube   The QIAGEN QIAcube Accelerated Validation Support service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the QIAcube system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on user support ａnd QIAcube instrument operation. The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Please indicate which protocols to include in the validation (QIAamp, QIAamp DNA Investigator, MinElute PCR Cleanup, etc.).       CNY                           

9242204  Adapter, 2x Rotor-Disc, Qsym             CNY                           

5000-1020R    DML 2000 Luminometer, 240V (Refurbished)            CNY                           

5000-1323      Rotary Shaker - Cord Line, 220V         CNY                           

5000-1317      Knob Speed Timer, SKR1             CNY                           

5000-1272      X Drive Track, DML2          CNY                           

5000-1260      Stepper Model New Styl, DML2          CNY                           

5001-2240      Hybrid Capture 2 (hc2)System Core, 240V         CNY                           

5000-1506      Switch ON-OFF 240V, HTR1              CNY                           

6000-4111ENV2.2  RCS User Application Manual (English)        CNY                           

6000-3117      Plate Washer I - Maintenance Kit          CNY                           

5199-1220      hc2 High-Risk HPV DNA Test             CNY                           

5126-1220      hc2 DNA Collection Device          CNY                            【人水通道蛋白4（AQP-4-AB）抗體elisa現(xiàn)貨】

GR8RNA QIAshredder (250)/RNeasy (250) spezial            CNY                           

RNEZ4U QIAshredder (50)/RNeasy (50) special        CNY                           

9240736  Decon tube, Actril, AP98              CNY                           

5000-1410      Strip Shock Absorbing, VTX2             CNY                           

9240692  Fan, 12Vdc, PC-104 cover, AP196/98         CNY                           

51331     QIAamp 96 DNA QIAcube HT Kit (5)         CNY                           

74171     RNeasy 96 QIAcube HT Kit (5)           CNY                           

180994    GeneRead Adapter L Set 12-plex (72)          CNY                           

       └ 虹膜色素上皮細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 晶狀體上皮細(xì)胞總DNA 1μg/1μl（10μg/20ug/50ug）                               【人水通道蛋白4（AQP-4-AB）抗體elisa現(xiàn)貨】

       └ 角膜上皮細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 角膜內(nèi)皮細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 視網(wǎng)膜神經(jīng)節(jié)細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 淋巴內(nèi)皮細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 淋巴成纖維細(xì)胞總DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 外周血白細(xì)胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       肺大動(dòng)脈內(nèi)皮細(xì)胞       5 × 105       1ml                      

       肺大動(dòng)脈平滑肌細(xì)胞    5 × 105       1ml                      

       肺大靜脈內(nèi)皮細(xì)胞       5 × 105       1ml                      

       肺大靜脈平滑肌細(xì)胞    5 × 105       1ml                      

       肺動(dòng)脈成纖維細(xì)胞       5 × 105       1ml                      

       肺泡上皮細(xì)胞       5 ×105  1ml                      

       氣管上皮細(xì)胞       5 × 105       1ml                      

       肺微血管內(nèi)皮細(xì)胞       5 × 105       1ml                      

       肺成纖維細(xì)胞       5 × 105       1ml                      

       心肌細(xì)胞       5 ×105  1ml                       【人水通道蛋白4（AQP-4-AB）抗體elisa現(xiàn)貨】

       心肌成纖維細(xì)胞    5 × 105       1ml                      

       主動(dòng)脈內(nèi)皮細(xì)胞    5 × 105       1ml                      

       主動(dòng)脈平滑肌細(xì)胞       5 × 105       1ml                      

       單核細(xì)胞       5 × 105       1ml                      

       大隱靜脈平滑肌細(xì)胞    5 × 105       1ml                      

       食管上皮細(xì)胞       5 × 105       1ml                      

       食管平滑肌細(xì)胞    5 × 105       1ml                      

       腸動(dòng)脈內(nèi)皮細(xì)胞    5 × 105       1ml                      

       腸靜脈內(nèi)皮細(xì)胞    5 × 105       1ml                      

       腸平滑肌細(xì)胞       5 × 105       1ml                      

       腸粘膜上皮細(xì)胞    5 × 105       1ml                      

       肝實(shí)質(zhì)細(xì)胞    5 ×105  1ml                      

       肝動(dòng)脈內(nèi)皮細(xì)胞    5 × 105       1ml                      

       肝動(dòng)脈平滑肌細(xì)胞       5 × 105       1ml                      

       肝內(nèi)膽管上皮細(xì)胞       5 × 105       1ml