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人α乳清蛋白(α-La)ELISA試劑盒廠家

點(diǎn)擊次數(shù):17發(fā)布時(shí)間:2016/8/17 16:48:23

人α乳清蛋白(α-La)ELISA試劑盒廠家

更新日期:2016/8/17 16:48:23

所 在 地:中國大陸

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簡單介紹:人α乳清蛋白(α-La)ELISA試劑盒廠家 貨號QY-H11896 規(guī)格48T/96T 樣本血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等 齊一生物科技(上海)有限公司銷售:021-6034 8496;181214 53965;173021 04490

相關(guān)標(biāo)簽:人α乳清蛋白(α-La)ELISA試劑盒廠家 

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詳細(xì)內(nèi)容

 人α乳清蛋白(α-La)ELISA試劑盒廠家ELISA 樣本的處理

ELISA 檢測的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性,在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。齊一生物銷售:0216034 8496;181214 53965;173021 04490

 

注意事項(xiàng):

   每個(gè)標(biāo)本量收集體積=  100ul × 檢測種類,如要做復(fù)孔,標(biāo)本量收集體積=  100ul × 檢測種類×2 。

   對于作某一個(gè)具體指標(biāo)來說,先用一系列稀釋度作一批標(biāo)本測定,得出對實(shí)驗(yàn)有意義的一個(gè)稀釋度(即測定劑量反應(yīng)曲線),然后用一個(gè)*適稀釋度測定即可。

   收集標(biāo)本前必須清楚要檢測的成份是否足夠穩(wěn)定。對收集后當(dāng)天進(jìn)行檢測的標(biāo)本,儲存在4℃?zhèn)溆茫缬刑厥庠蛐枰芷谑占瘶?biāo)本,將標(biāo)本及時(shí)分裝后放在-20℃ 或-70℃ 條件下保存。避免反復(fù)凍融。標(biāo)本 2 -8 ℃ 可保存48 小時(shí).-20℃ 可保存1個(gè)月。 -70 度可保存6個(gè)月。部分激素類標(biāo)本需添加抑肽酶。

   血清標(biāo)本采集時(shí),應(yīng)注意避免溶血,紅細(xì)胞溶解時(shí)會釋放出具有過氧化物酶活性的物質(zhì),以 HRP為標(biāo)記的 ELISA 測定中,溶血標(biāo)本可能會增加非特異性顯色。

人α乳清蛋(α-La)ELISA試劑盒廠家   為了保證尿液檢測結(jié)果的準(zhǔn)確性,必須正確收集尿液標(biāo)本和保存。盛尿容器要清潔干燥。使用一次性的容器(如塑料尿杯),避免因用藥并清洗不干凈而造成的污染,影響檢測結(jié)果。尿液標(biāo)本必須新鮮,留取后,應(yīng)及時(shí)檢測或保存,以免細(xì)菌繁殖。因在室溫中久置后(尤其是夏季)尿中磷酸鹽等可析出結(jié)晶而干擾檢測。

    標(biāo)本宜在新鮮時(shí)檢測。如有細(xì)菌污染,菌體中可能含有內(nèi)源性 HRP,也會產(chǎn)生假陽性反應(yīng)。保存過久可發(fā)生聚合在 ELISA中可使本底加深。

   凍結(jié)標(biāo)本融解后,蛋白質(zhì)局部濃縮,分布不均,應(yīng)充分混勻宜輕緩,避免氣泡,可上下顛倒混和,不要在混勻器上強(qiáng)烈振蕩。

   混濁或有沉淀的標(biāo)本應(yīng)先離心或過濾,澄清后再檢測。

   反復(fù)凍融會使蛋白效價(jià)降低,所以待測標(biāo)本如需保存作多次檢測,宜少量分裝冰存。也可加入適當(dāng)防腐劑。

   如果您在實(shí)驗(yàn)中遇到任何特殊問題,敬請咨詢 021-60348496標(biāo)本類型

ELISA常見的標(biāo)本一般包括:

   液體類標(biāo)本:包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清等。

   培養(yǎng)細(xì)胞

   組織標(biāo)本

這些標(biāo)本收集的時(shí)間、方法和保存都有一定的要求。

人α乳清蛋白(α-La)ELISA試劑盒廠家標(biāo)本的保存

一般說來,在 5 天內(nèi)測定的標(biāo)本可放置于 4℃,超過一周測定的需低溫凍存。

對收集后當(dāng)天進(jìn)行檢測的標(biāo)本,儲存在  4 ℃ 備用,如有特殊原因需要周期收集標(biāo)本, 將標(biāo)本及時(shí)分裝后放在  -20 ℃ 或  -70 ℃ 條件下保存。避免反復(fù)凍融。 標(biāo)本  2 -8 ℃ 可保存  48 小時(shí),-20 ℃ 可保存  1 個(gè)月。-70 度可保存  6 個(gè)月。部分標(biāo)本也可加入適當(dāng)防腐劑,激素類標(biāo)本需添加抑肽酶。標(biāo)本的處理 可用作 ELISA 測定的標(biāo)本十分廣泛,體液(如血清)、分泌物(唾液)和排泄物(如尿液)、細(xì)胞培養(yǎng)上清、細(xì)胞、組織等均可作標(biāo)本以測定其中某種成份。有些標(biāo)本可直接進(jìn)行測定,有些則需經(jīng)預(yù)處理。

◆ 血清:  

室溫血液自然凝固  10-20 分鐘后,離心  20 分鐘左右(  2000-3000 轉(zhuǎn)  / 分)。收集上清。如有沉淀形成,應(yīng)再次離心。  

◆ 血漿:  

應(yīng)根據(jù)試劑盒的要求選擇  EDTA 、檸檬酸鈉或肝素作為抗凝劑,加入  10 %(  v/v )抗凝劑 ( 0.1M 檸檬酸鈉或  1% heparin   2.0%EDTA.Na2) 混合  10-20 分鐘后,離心  20 分鐘左右(  2000-3000 轉(zhuǎn)  / 分)。仔細(xì)收集上清如有沉淀形 成,應(yīng)再次離心。  

人α乳清蛋白(α-La)ELISA試劑盒廠家◆ 尿液、胸腹水、腦脊液:  

用無菌管收集。離心  20 分鐘左右(  2000-3000 轉(zhuǎn)  / 分)。仔細(xì)收集上清。如有沉淀形成,應(yīng)再次離心。  

◆ 細(xì)胞培養(yǎng)上清:  

檢測分泌性的成份時(shí),用無菌管收集。離心  20 分鐘左右(  2000-3000 轉(zhuǎn)  / 分)。仔細(xì)收集上清。檢測細(xì)胞內(nèi)的 成份時(shí),用  PBS   PH7.2-7.4 )稀釋細(xì)胞懸液,細(xì)胞濃度達(dá)到  100 /ml 左右。通過反復(fù)凍融,以使細(xì)胞破壞并放出細(xì)胞內(nèi)成份。離心  20 分鐘左右(  2000-3000 轉(zhuǎn) / 分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。  

◆ 細(xì)胞:

檢測細(xì)胞的成份時(shí),對于貼壁細(xì)胞,去除培養(yǎng)液,用PBS、生理鹽水或無血清培養(yǎng)液洗一遍。加入適量裂解液。用槍吹打數(shù)下,使裂解液和細(xì)胞充分接觸。通常裂解液接觸細(xì)胞10 秒后,細(xì)胞就會被裂解。對于懸浮細(xì)胞,離心收集細(xì)胞,用PBS、生理鹽水或無血清培養(yǎng)液洗一遍.加入適量裂解液, 用槍吹打把細(xì)胞吹散。用手指輕彈以充分裂解細(xì)胞。充分裂解后應(yīng)沒有明顯的細(xì)胞沉淀。如果細(xì)胞量較多,必需分裝然后再裂解。充分裂解后,10000-14000g離心3-5 分鐘,取上

清,即可進(jìn)行后續(xù)操作。

 

人α乳清蛋白(α-La)ELISA試劑盒廠家◆ 組織標(biāo)本:  

切割標(biāo)本后,稱取重量。加入一定量的  PBS或組織蛋白萃取試劑,緩沖液中可加入蛋白酶抑制劑。用手工或勻漿器將標(biāo)本勻漿充分。離心  20 分鐘左右(  2000-3000 轉(zhuǎn)  / 分)。仔細(xì)收集上清置于  -20 度或  - 70 度保存,如有必要,可以將樣品濃縮干燥。分裝后一份待檢測,其余冷凍備用。齊一生物銷售:0216034 8496181214 53965;173021 04490  

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ULBC plates    超低結(jié)合培養(yǎng)板(6孔)     Ultra-Low Binding Culture Plate (6-well)     "6-well, 4 plates

"                           【人α乳清蛋白(α-La)ELISA試劑盒廠家】

ULBC plate     超低結(jié)合培養(yǎng)板(24孔)   Ultra-Low Binding Culture Plate (24-well)   "24-well, 1 plate

"                          

ULBC plates    超低結(jié)合培養(yǎng)板(24孔)   Ultra-Low Binding Culture Plate (24-well)   "24-well, 4 plates

"                          

rhbFGF   重組人堿性成纖維細(xì)胞生長因子--堿性     Recombinant Human Fibroblast Growth Factor-basic  10µg                           

rhEGF     重組人表皮生長因子    "Recombinant Human Epidermal Growth Factor

"      100µg                         

rhBMP-2 重組人骨形成蛋白-2    "Recombinant Human Bone Morphogenetic Protein-2

"      2µg                      

rhBMP-4 重組人骨形成蛋白-4    "Recombinant Human Bone Morphogenetic Protein-4

"      2µg                      

rhBMP-7 重組人骨形成蛋白-7    "Recombinant Human Bone Morphogenetic Protein-7

"      2µg                      

rhNOGGIN    重組人NOGGIN蛋白  "Recombinant Human NOGGIN

"      5µg                      

HAMSC  人羊膜間充質(zhì)基質(zhì)細(xì)胞       Human Amniotic Mesenchymal Stromal Cells      5 x 10^5 cells/vial                       

HCMSC  人絨毛膜間充質(zhì)基質(zhì)細(xì)胞    Human Chorionic Mesenchymal Stromal Cells    5 x 10^5 cells/vial                       

HMSC-bm      人間充質(zhì)干細(xì)胞--骨髓 Human Mesenchymal Stem Cells-bone marrow   5 x 10^5 cells/vial                       

HMSC-ad       人間充質(zhì)干細(xì)胞--脂肪 Human Mesenchymal Stem Cells-adipose     5 x 10^5 cells/vial                       

HMSC-hp       人間充質(zhì)干細(xì)胞--肝臟 Human Mesenchymal Stem Cells-hepatic     5 x 10^5 cells/vial                       

HUMSC  人臍帶間充質(zhì)干細(xì)胞    Human Umbilical Mesenchymal Stem Cells   5 x 10^5 cells/vial                       

HPMSC   人肺間充質(zhì)干細(xì)胞       Human Pulmonary Mesenchymal Stem Cells       5 x 10^5 cells/vial                       

HVMSC  人脊椎間充質(zhì)干細(xì)胞    Human Vertebral Mesenchymal Stem Cells   5 x 10^5 cells/vial                        【人α乳清蛋白(α-La)ELISA試劑盒廠家】

MSCM    間充質(zhì)干細(xì)胞培養(yǎng)基  Mesenchymal Stem Cell Medium   500 ml                        

MODM-500    間充質(zhì)干細(xì)胞-成骨細(xì)胞分化培養(yǎng)基 Mesenchymal Stem Cell Oseogenic Differentiation Medium 500 ml                        

MODM-250           Mesenchymal Stem Cell Oseogenic Differentiation Medium 250 ml                        

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MADM-250           Mesenchymal Stem Cell Adipogenic Differentiation Medium       250 ml                        

MADM-b       間充質(zhì)干細(xì)胞-脂肪細(xì)胞分化培養(yǎng)基-基礎(chǔ) Mesenchymal Stem Cell Adipogenic Differentiation Medium-basal        500 ml                        

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Ared       茜素紅S染色試劑盒    Alizarin Red S Staining Kit     100 ml                        

Ored       油紅O染色試劑盒       Oil Red O Staining Kit    100 ml                        

PCR 人間質(zhì)干細(xì)胞成脂肪細(xì)胞檢測試劑盒       Human Mesenchymal Stem Cell Adipogenesis Detection Kit       50 reactions                        

HMSC-C PCR 人間充質(zhì)干細(xì)胞軟骨形成檢測試劑盒       Human Mesenchymal Stem Cell Chondrogenesis Detection Kit   50 reactions                            

SafraninO       番紅O染色試劑盒       Safranin O Staining Kit   100 reactions                            

ABlue      阿利新藍(lán)染色試劑盒    Alcian Blue Staining Kit  100 reactions                             【人α乳清蛋白(α-La)ELISA試劑盒廠家】

s�ci�*�)ily:"Times New Roman";Times New Roman"'>人真皮成纖維細(xì)胞-胎兒C處理  Human Dermal Fibroblasts-Fetal-Mitomycin C treated 1 x 10^6 cells/vial                      

 

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bFGF-std MEF-cm 堿性成纖維細(xì)胞生長因子刺激的小鼠胚胎成纖維細(xì)胞條件培養(yǎng)基       bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium   100ml                         

ULBC plates    干細(xì)胞超低結(jié)合培養(yǎng)板       Ultra-Low Binding Culture Plate    6-well, 1 plate                          

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5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer and each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

 

 

Storage and validity

1Storage  2-8.

2validity six months.

9237124       Upgrade App., MinElute 96       Take advantage of our instrumentation and molecular biology expertise to install MinElute 96  on your BioRobot System.  The onsite protocol development visit includes all travel and labor for the visit to install the standard QIAGEN MinElute 96 protocol, plus a MinElute 96 BioRobot Kit (4-plate) Cat # 28051 CNY                           

9237099       Upgrade BR configuration, on site            CNY                          

9236424       Motor, rotary, TWI             CNY                          

9232970       YZ 96 Connector Board 2 (Arm), E10103            CNY                          

9236409       Plate Stacker TW I, microplate         CNY                          

9236357       Sample Holder Large, 1.5ml             CNY                           【人淀粉樣前體蛋白(βAPP)ELISA試劑盒廠家】

9232911       Board, YZ driver         CNY                          

9236336       Analog Board              CNY                          

9236158       Pot. , syr. or vert. , kit, RapidPlate           CNY                          

9236149       Locator Plate, 96 well,pos 4-5,BR RP            CNY                          

9236114       O-Ring, Coupling, RoboVac, BR8 RD v4.2           CNY                          

9236107       Flange Tool          CNY                          

9230680       Probe, 0.4 mm, BR8000 v1.01         CNY                          

9230478       Rack f. Diamend Gel Cards (holds 6cards)            CNY                          

9230473       Set, Cables, PCB (X-main) to shaker             CNY                          

9235646       Connector, Board, HS shaker, BR8000 v1.0         CNY                          

9235255       T-piece, HSPS tubing, connector             CNY                          

9235190       Motor, clamp, shaker plate,  BR8000           CNY                          

9230060       Rack for reagents (12 x diameter 21mm)         CNY                          

9238673       Hinge, imbalance system            CNY                          

9238653       Power Board, temp. switch - heat sink            CNY                          

9238635       Hook identification compl.         CNY                           【人淀粉樣前體蛋白(βAPP)ELISA試劑盒廠家】

9242028       RGQ, Repair Fee "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: 0

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"    CNY                          

9241821       Pyro Q96 MDa, Basic Agreement    "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"  CNY                          

9241786       QIAgility Demopool Box Light Version           CNY                          

9240976       PCB, display, sample tracking, Qsym              CNY                          

9240968       Barcode camera,  sample tracking, Qsym             CNY                          

9240816       #Full Agreement, EZ1 DSP, 1YR             CNY                          

9240680       Spill Tray, Shaker,Modified, AP        CNY                          

9240790       Update,Waste Bottle Sensor, AP196/98         CNY                          

6000-5014    Lumicheck Plate Service Package, DML2             CNY                          

TGS       滋養(yǎng)層細(xì)胞生長因子     Trophoblast Growth Supplement       5 ml                     

PAdDS  前脂肪細(xì)胞分化生長因子     Preadipocyte Differentiation Supplement                             

PAGS    前脂肪細(xì)胞生長因子     Preadipocyte Growth Supplement     5 ml                      【人淀粉樣前體蛋白(βAPP)ELISA試劑盒廠家】

AdGS    脂肪細(xì)胞生長因子   Adipocyte Growth Supplement  5 ml                     

OEpiCGS     卵巢上皮細(xì)胞生長因子  Ovarian Epithelial Cell Growth Supplement     5 ml                     

MODS   間充質(zhì)干細(xì)胞成骨分化生長因子       Mesenchymal Stem Cell Osteogenic Differentiation supplement    5 ml                     

MADS   間充質(zhì)干細(xì)胞脂肪分化生長因子       Mesenchymal Stem Cell Adipogenic Differentiation supplement    5 ml                     

MSCGS 間充質(zhì)干細(xì)胞生長因子  Mesenchymal Stem Cell Growth Supplement  5 ml                     

MSCGS-sf    間充質(zhì)干細(xì)胞生長因子  Mesenchymal Stem Cell Growth Supplement-serum free      5 ml                     

MSCGS-acf  間充質(zhì)干細(xì)胞生長因子-無動物組分  Mesenchymal Stem Cell Growth Supplement-animal component free 5 ml                     

MCDS   間充質(zhì)干細(xì)胞軟骨分化生長因子       Mesenchymal Stem Cell Chondrogenic Differentiation Supplement      5 ml                        

MEpiCGS     乳腺上皮細(xì)胞生長因子       Mammary Epithelial Cell Growth Supplement  5 ml                     

FBS       胎牛血清     Fetal Bovine Serum, Human Cell Tested   10 ml                          

FBS       胎牛血清     Fetal Bovine Serum, Human Cell Tested   25 ml                          

T/E  胰酶/EDTA消化液   Trypsin/EDTA solution 100 ml                        

TNS       胰酶中和液 Trypsin Neutralization Solution   100 ml                        

TNS-sf 胰酶中和液-無血清  TNS-sf (Trypsin Neutralization Solution-serum free)      100 ml                        

ECDS    非酶細(xì)胞裂解液       Enzyme-Free Cell Dissociation Solution   100 ml                        

CFM      細(xì)胞凍存液 Cell Freezing Medium  50 ml                           【人淀粉樣前體蛋白(βAPP)ELISA試劑盒廠家】

SF-CFM       無血清細(xì)胞凍存液        Serum-Free Cell Freezing Medium    50 ml                          

StemCryo      人胚胎干細(xì)胞凍存液      Human Pluripotent Stem Cell Cryopreservation Medium       50ml                           

StemCryo      人胚胎干細(xì)胞凍存液      Human Pluripotent Stem Cell Cryopreservation Medium,      5 x 10mL                          

TB  臺盼藍(lán)  Trypan Blue, 0.4%       50 ml                          

DPBS    磷酸鹽緩沖液   Dulbeccos Phosphate-Buffered Saline  500 ml                        

HBSS    平衡鹽溶液       Hank's Balanced Salt Solution    500 ml                        

PLL       多聚賴氨酸       Poly-L-Lysine 1 mg/ml 1 ml                     

PLL       多聚賴氨酸       Poly-L-Lysine 10 mg/ml      1 ml                     

GSN      0.2%的明膠溶液      0.2% Gelatin Solution  100ml                         

FBS       胎牛血清    Fetal Bovine Serum, Human Cell Tested   500 ml                        

P/S  青霉素/鏈霉素溶液        Penicillin/Streptomycin Solution  5 ml                     

P/S  青霉素/鏈霉素溶液        Penicillin/Streptomycin Solution  100 ml                        

AMS      抗真菌溶液       Antimycotic Solution    50 ml                          

ABAMS       抗菌/抗霉菌溶液      Antibiotic/Antimycotic Solution  50 ml                           

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