ELISA酶聯(lián)法試劑盒操作因固相載體的形成不同而有所差異，國內(nèi)醫(yī)學(xué)檢驗一般均用板式點。本文將敘述板式ELISA各個操作步驟的注意要點，珠式、管式及磁性球ELSIA，國外試劑均與特殊儀器配合應(yīng)用，兩者均有詳細(xì)的使用說明，嚴(yán)格遵照規(guī)定操作，必能得出準(zhǔn)確的結(jié)果。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

首先齊一生物優(yōu)質(zhì)的試劑盒，良好的儀器和正確的操作是保證ELISA檢測結(jié)果準(zhǔn)確可靠必要條件。所以ELISA試劑盒的購買，試劑質(zhì)量的保證以及實驗前實驗儀器的檢察都是很有重要性的。保證實驗材料的質(zhì)量之后要嚴(yán)格按照實驗步驟進(jìn)行。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人β2糖蛋白(β2-GP)ELISA試劑盒現(xiàn)貨】齊一生物ELISA試劑盒

1.精心挑選的進(jìn)口抗體、標(biāo)準(zhǔn)品、顯色劑和酶標(biāo)板全部為進(jìn)口產(chǎn)品實現(xiàn)*佳性能；

2.原材料批量進(jìn)口加上自配的特殊配方稀釋劑保證高品質(zhì)產(chǎn)品性能的同時兼顧成本；

3.采用技術(shù)，嚴(yán)格按照標(biāo)準(zhǔn)生產(chǎn)方案組裝生產(chǎn)；

4.預(yù)包被微孔板的批內(nèi)和批間變異系數(shù)均小于10 ％；

5.經(jīng)過嚴(yán)格交叉反應(yīng)和干擾測試及穩(wěn)定性試驗；

6.明確的敏感度；

7.針對所有樣品類型達(dá)到*佳的性能；

8.廣泛的檢測范圍可以滿足大多數(shù)檢測要求；

9.完善的售后服務(wù)和免費的技術(shù)支持免去您的后顧之憂；

10.強(qiáng)大的生產(chǎn)能力和充足的儲備、操作熟練的技術(shù)人員及高效的生產(chǎn)流程，隨時可以組裝出您定制的產(chǎn)品。

【人β2糖蛋白(β2-GP)ELISA試劑盒現(xiàn)貨】齊一生物僅供實驗人、大鼠、小鼠、兔等動物實驗標(biāo)本檢測使用！得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！齊一生物銷售：021－6034 8496；181214 53965；173021 04490

ELISA試劑盒問題描述

標(biāo)準(zhǔn)曲線梯度差：

吸液或加液不準(zhǔn)請檢查移液器及吸頭

標(biāo)準(zhǔn)品稀釋不正確溶解標(biāo)準(zhǔn)品稍微旋轉(zhuǎn)瓶身，輕輕混勻使粉末完全溶解

洗滌不完全保證洗滌時間和洗滌次數(shù)及每孔的加液量

顯色很弱或無色：

孵育時間太短保證充足的孵育時間

實驗溫度不正確使用推薦的實驗溫度

試劑體積不夠或漏加、稀釋不正確檢查吸液及加液過程，保證所有試劑按順序足量添加

讀數(shù)數(shù)值低：

酶標(biāo)儀設(shè)置不正確 在酶標(biāo)儀上檢查波長及濾光片設(shè)置

提前打開酶標(biāo)儀預(yù)熱：變異系數(shù)大加液不正確檢查加液情況

【人β2糖蛋白(β2-GP)ELISA試劑盒現(xiàn)貨】背景值高：

檢測抗體的工作濃度使用推薦的稀釋倍數(shù)

酶標(biāo)板洗滌不完全重新閱讀操作手冊，保證清洗完全；如果用自動洗板機(jī)，請檢查所有的出口是否有堵塞

洗液有污染配制新鮮的洗液

靈敏度低：

ELISA試劑盒保存按說明書要求保存相關(guān)試劑

讀數(shù)前未終止OD讀數(shù)前應(yīng)在每孔中加入終止液

齊一生物客戶寄標(biāo)本時需注明以下情況：

1、標(biāo)本編號；2、所測項目；3、是否做復(fù)孔；3、聯(lián)系方式；4、實驗后標(biāo)本是否寄回。

客戶必知：

客戶應(yīng)對所提供的材料及信息負(fù)責(zé)，如因客戶提供的材料及信息不準(zhǔn)確而引起的實驗延誤或經(jīng)濟(jì)損失由客戶承擔(dān)。

試劑盒樣本要求

在收集標(biāo)本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測，對收集后當(dāng)天就進(jìn)行檢測的標(biāo)本，及時儲存在4℃?zhèn)溆�，如有特殊原因需要周期收集�?biāo)本，請造模取材后，將標(biāo)本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質(zhì)量，所以避免反復(fù)凍融。具體操作注意事項請與我司技術(shù)人員溝通。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人β2糖蛋白(β2-GP)ELISA試劑盒現(xiàn)貨】液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

1. 血清：室溫血液自然凝固10-20分鐘后，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。

2. 血漿：應(yīng)根據(jù)標(biāo)本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑，混合10-20分鐘后，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成應(yīng)再次離心。

3. 尿液、胸腹水、腦脊液：用無菌管收集。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。如有沉淀形成，應(yīng)再次離心。

4. 細(xì)胞培養(yǎng)上清：檢測分泌性的成份時，用無菌管收集。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。

5. 培養(yǎng)細(xì)胞:檢測細(xì)胞內(nèi)的成份時，用PBS（PH7.2-7.4）稀釋細(xì)胞懸液，細(xì)胞濃度達(dá)到100萬/ml左右。通過反復(fù)凍融或加入組織蛋白萃取試劑，以使細(xì)胞破壞并放出細(xì)胞內(nèi)成份。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。

6. 組織標(biāo)本：切割標(biāo)本后，稱取重量。加入一定量的PBS，緩沖液中可加入1μg/L蛋白酶抑制劑或50U/ml的Aprotinin（抑肽酶）。用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清置于-20度或-70度保存，如有必要，可以將樣品濃縮干燥。分裝后一份待檢測，其余冷凍備用。

7. 標(biāo)本采集后盡早進(jìn)行提取，提取按相關(guān)文獻(xiàn)進(jìn)行，提取后應(yīng)盡快進(jìn)行實驗。若不能馬上進(jìn)行試驗，可將標(biāo)本放于-20℃保存，但應(yīng)避免反復(fù)凍融

8.不能檢測含NaN3的樣品，因NaN3抑制辣根過氧化物酶的（HRP）活性。

9.如果樣品不能立即檢測，應(yīng)將其分裝，-70 ℃保存，避免反復(fù)冷凍。保存過程中如出現(xiàn)沉淀，應(yīng)再次離心。血液標(biāo)本盡可能的不要使用溶血或高血脂血。如果血清中含大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解

凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

9241770  QIAxtractor, Premium Agreement The Premium Cover Agreement, QIAxtractor provides full service coverage for the QIAxtractor ａnd the CAS 1280. Costs for service visits are fully covered including travel, labor, ａnd parts. One on-site pipetting head service ａnd inspection is included. With a Premium Cover Agreement, there is a priority response time of 24 hours (1 working day).       CNY                           

9238971  Target Prep, Full Agreement  The Full Agreement TargetPrep provides full service coverage for the BioRobot TargetPrep. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).       CNY                           

9238953  Vertical plate, BS15        CNY                           

9238792  Angle rotor for 12 Falcon tubes15 ml          CNY                           

9017118  LiquiChip IS 200, Plate Handler            CNY                           

9232556  Vacuum manifold, strip holder 1.02             CNY                           

9232477  Lid, wash station, BR8000 v1.01          CNY                            【人β2糖蛋白(β2-GP)ELISA試劑盒現(xiàn)貨】

9238940  PCB, CSU-02, BS15             CNY                           

9238783  Swing-out rotor for 4 buckets      Sigma Rotor # 11150     CNY                           

9238914  Shield motor, BS96        CNY                           

9238705  MP-motor capacitor             CNY                           

9231890  Base, 1-8 connector, BR8000        CNY                           

9238688  PC board Zent1, no Eprom, parameter free         CNY                           

9231133  Flat Band Cable 10P 1170mm Xmain-Xadp          CNY                           

9020069  Front plate, TLyser 2            CNY                           

9020058  Fuse T 0,63A, 220-240V 50/60Hz, TLyser          CNY                           

9014867  Valve, Pinch, RoboVac, BR8         CNY                           

9014599  PCB, YZ-Adapter, BR8          CNY                           

9014579  QIAVac Multiwell    Vacuum manifold for processing 96-well purification plates of SBS standard     CNY                           

9022581  PCB - Mainboard(progr.)Biocontrol,RGQII         CNY                           

9022409  RCS Tip Eject Station v.2             CNY                           

9022130  Optical Head Assembly, careHPV         CNY                           

9022125  Tool, Thermistor Gauge, RGx              CNY                            【人β2糖蛋白(β2-GP)ELISA試劑盒現(xiàn)貨】

9021983  Latching mechanism (2), top lids, QXA              CNY                           

9021290  Silicone grease for PM, EZ1         CNY                           

9238617  Magnet, tacho        CNY                           

9238473  Rotary flex circuit, BR RapidPlate        CNY                           

9238240  BioRobot M48 User Manual          CNY                           

9238225  Sheath Delivery Tubing w. Connectors        CNY                           

9238158  Heat Transfer Adapter, microplate FB          CNY                           

9240660  Block, Plastic, QC, AP96/196              CNY                           

CCGW    細(xì)胞培養(yǎng)超純水   Cell Culture Grade Water       500 ml                        

MDS     肌細(xì)胞分離液       Myocyte Detachment Solution      100 ml                         【人β2糖蛋白(β2-GP)ELISA試劑盒現(xiàn)貨】

BPE 牛腦垂體提取物   Bovine Pituitary Extract  25 mg                         

BPE 牛腦垂體提取物   Bovine Pituitary Extract  100 mg                       

ITS  胰島素鐵硒傳遞蛋白    Insulin-Transferrin-Selenium, 100x      10 ml                          

L-Glu      左旋谷氨酰胺溶液      L-Glutamine Solution, 200 mM     100 ml                        

NEAA     100X非必需氨基酸      100X Non-Essential Amino Acids  100ml                         

HGL PCR       人胚層檢測試劑盒       Human Germ Layer Detection Kit  50 reactions                       

Ored       油紅O染色試劑盒       Oil Red O Staining Kit    100 ml                        

StemDS  人胚胎干細(xì)胞分離液    StemDS® Human Embryonic Stem Cell Dissociation Solution    100 ml                        

BPF 牛血漿纖維粘連蛋白    Bovine Plasma Fibronectin     1 mg                           

HCF 人細(xì)胞纖連蛋白    Human Cellular Fibronectin    100 μg                      

BPV 牛血漿玻連蛋白    Bovine Plasma Vitronectin    100 μg                      

rHSA      重組人血清白蛋白       "Recombinant Human Serum Albumin

"      1g                        

BPF 牛血漿纖維粘連蛋白    Bovine Plasma Fibronectin     1 mg                           

CCCSCK 膠原I-細(xì)胞培養(yǎng)表面包被試劑盒      Collage I-Cell Culture Surface Coating Kit    10 mg                         

PLL 多聚賴氨酸   Poly-L-Lysine 1 mg/ml   1 ml                     

PLL 多聚賴氨酸   Poly-L-Lysine 10 mg/ml 1 ml                      【人β2糖蛋白(β2-GP)ELISA試劑盒現(xiàn)貨】

STEMium       人胚胎干細(xì)胞無血清培養(yǎng)基       Human Pluripotent Stem Cell Growth Medium     500 ml                        

STEMium-XF 無異種人胚胎干細(xì)胞生長培養(yǎng)基       Xeno-free Human Pluripotent Stem Cell Growth Medium    500 ml                        

STEMium-ACF      無動物成分的人胚胎干細(xì)胞生長培養(yǎng)基    Animal Component-free Human Pluripotent Stem Cell Growth Medium   500 ml                        

StemDS  人胚胎干細(xì)胞分離液    StemDS® Human Embryonic Stem Cell Dissociation Solution    100 ml                        

StemCryo       人胚胎干細(xì)胞凍存液    Human Pluripotent Stem Cell Cryopreservation Medium      50ml                           

StemCryo       人胚胎干細(xì)胞凍存液    Human Pluripotent Stem Cell Cryopreservation Medium,     5 x 10mL                           

MEF       小鼠胚胎成纖維細(xì)胞    Mouse Embryonic Fibroblasts       5 x 10^5 cells/vial                       

MEF-mt  小鼠胚胎成纖維細(xì)胞－－處理 Mouse Embryonic Fibroblasts-mitomicine treated       5 x 10^5 cells/vial                       

MEF-mt  小鼠胚胎成纖維細(xì)胞－－CD1小鼠C處理    Mouse Embryonic Fibroblasts- Mitomycin C treated from CD1 mouse     1 x 10^6 cells/vial                     

MEF-mt  小鼠胚胎成纖維細(xì)胞－－CF1小鼠C處理     Mouse Embryonic Fibroblasts from CF1 mouse 1 x 10^6 cells/vial                       

MEF-mt  小鼠胚胎成纖維細(xì)胞－－CF1小鼠C處理     Mouse Embryonic Fibroblasts-Mitomycin C treated from CF1 mouse      1 x 10^6 cells/vial                     

HDF-f-mt       人真皮成纖維細(xì)胞-胎兒C處理  Human Dermal Fibroblasts-Fetal-Mitomycin C treated 1 x 10^6 cells/vial                      

MEF-cm 小鼠胚胎成纖維細(xì)胞培養(yǎng)基       Mouse Embryonic Fibroblasts-Conditioned Medium    100 ml                        

bFGF-std MEF-cm 堿性成纖維細(xì)胞生長因子刺激的小鼠胚胎成纖維細(xì)胞條件培養(yǎng)基       bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium   100ml                         

ULBC plates    干細(xì)胞超低結(jié)合培養(yǎng)板       Ultra-Low Binding Culture Plate    6-well, 1 plate                          

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9237124       Upgrade App., MinElute 96       Take advantage of our instrumentation ａnd molecular biology expertise to install MinElute 96  on your BioRobot System.  The onsite protocol development visit includes all travel ａnd labor for the visit to install the standard QIAGEN MinElute 96 protocol, plus a MinElute 96 BioRobot Kit (4-plate) Cat # 28051 CNY                           

9237099       Upgrade BR configuration, on site            CNY                          

9236424       Motor, rotary, TWI             CNY                          

9232970       YZ 96 Connector Board 2 (Arm), E10103            CNY                          

9236409       Plate Stacker TW I, microplate         CNY                          

9236357       Sample Holder Large, 1.5ml             CNY                           【人淀粉樣前體蛋白(βAPP)ELISA試劑盒廠家】

9232911       Board, YZ driver         CNY                          

9236336       Analog Board              CNY                          

9236158       Pot. , syr. or vert. , kit, RapidPlate           CNY                          

9236149       Locator Plate, 96 well,pos 4-5,BR RP            CNY                          

9236114       O-Ring, Coupling, RoboVac, BR8 RD v4.2           CNY                          

9236107       Flange Tool          CNY                          

9230680       Probe, 0.4 mm, BR8000 v1.01         CNY                          

9230478       Rack f. Diamend Gel Cards (holds 6cards)            CNY                          

9230473       Set, Cables, PCB (X-main) to shaker             CNY                          

9235646       Connector, Board, HS shaker, BR8000 v1.0         CNY                          

9235255       T-piece, HSPS tubing, connector             CNY                          

9235190       Motor, clamp, shaker plate,  BR8000           CNY                          

9230060       Rack for reagents (12 x diameter 21mm)         CNY                          

9238673       Hinge, imbalance system            CNY                          

9238653       Power Board, temp. switch - heat sink            CNY                          

9238635       Hook identification compl.         CNY                           【人淀粉樣前體蛋白(βAPP)ELISA試劑盒廠家】

9242028       RGQ, Repair Fee "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: 0

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"    CNY                          

9241821       Pyro Q96 MDa, Basic Agreement    "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"  CNY                          

9241786       QIAgility Demopool Box Light Version           CNY                          

9240976       PCB, display, sample tracking, Qsym              CNY                          

9240968       Barcode camera,  sample tracking, Qsym             CNY                          

9240816       #Full Agreement, EZ1 DSP, 1YR             CNY                          

9240680       Spill Tray, Shaker,Modified, AP        CNY                          

9240790       Update,Waste Bottle Sensor, AP196/98         CNY                          

6000-5014    Lumicheck Plate Service Package, DML2             CNY                          

TGS       滋養(yǎng)層細(xì)胞生長因子     Trophoblast Growth Supplement       5 ml                     

PAdDS  前脂肪細(xì)胞分化生長因子     Preadipocyte Differentiation Supplement                             

PAGS    前脂肪細(xì)胞生長因子     Preadipocyte Growth Supplement     5 ml                      【人淀粉樣前體蛋白(βAPP)ELISA試劑盒廠家】

AdGS    脂肪細(xì)胞生長因子   Adipocyte Growth Supplement  5 ml                     

OEpiCGS     卵巢上皮細(xì)胞生長因子  Ovarian Epithelial Cell Growth Supplement     5 ml                     

MODS   間充質(zhì)干細(xì)胞成骨分化生長因子       Mesenchymal Stem Cell Osteogenic Differentiation supplement    5 ml                     

MADS   間充質(zhì)干細(xì)胞脂肪分化生長因子       Mesenchymal Stem Cell Adipogenic Differentiation supplement    5 ml                     

MSCGS 間充質(zhì)干細(xì)胞生長因子  Mesenchymal Stem Cell Growth Supplement  5 ml                     

MSCGS-sf    間充質(zhì)干細(xì)胞生長因子  Mesenchymal Stem Cell Growth Supplement-serum free      5 ml                     

MSCGS-acf  間充質(zhì)干細(xì)胞生長因子-無動物組分  Mesenchymal Stem Cell Growth Supplement-animal component free 5 ml                     

MCDS   間充質(zhì)干細(xì)胞軟骨分化生長因子       Mesenchymal Stem Cell Chondrogenic Differentiation Supplement      5 ml                        

MEpiCGS     乳腺上皮細(xì)胞生長因子       Mammary Epithelial Cell Growth Supplement  5 ml                     

FBS       胎牛血清     Fetal Bovine Serum, Human Cell Tested   10 ml                          

FBS       胎牛血清     Fetal Bovine Serum, Human Cell Tested   25 ml                          

T/E  胰酶/EDTA消化液   Trypsin/EDTA solution 100 ml                        

TNS       胰酶中和液 Trypsin Neutralization Solution   100 ml                        

TNS-sf 胰酶中和液-無血清  TNS-sf (Trypsin Neutralization Solution-serum free)      100 ml                        

ECDS    非酶細(xì)胞裂解液       Enzyme-Free Cell Dissociation Solution   100 ml                        

CFM      細(xì)胞凍存液 Cell Freezing Medium  50 ml                           【人淀粉樣前體蛋白(βAPP)ELISA試劑盒廠家】

SF-CFM       無血清細(xì)胞凍存液        Serum-Free Cell Freezing Medium    50 ml                          

StemCryo      人胚胎干細(xì)胞凍存液      Human Pluripotent Stem Cell Cryopreservation Medium       50ml                           

StemCryo      人胚胎干細(xì)胞凍存液      Human Pluripotent Stem Cell Cryopreservation Medium,      5 x 10mL                          

TB  臺盼藍(lán)  Trypan Blue, 0.4%       50 ml                          

DPBS    磷酸鹽緩沖液   Dulbecco’s Phosphate-Buffered Saline  500 ml                        

HBSS    平衡鹽溶液       Hank＇s Balanced Salt Solution    500 ml                        

PLL       多聚賴氨酸       Poly-L-Lysine 1 mg/ml 1 ml                     

PLL       多聚賴氨酸       Poly-L-Lysine 10 mg/ml      1 ml                     

GSN      0.2％的明膠溶液      0.2% Gelatin Solution  100ml                         

FBS       胎牛血清    Fetal Bovine Serum, Human Cell Tested   500 ml                        

P/S  青霉素/鏈霉素溶液        Penicillin/Streptomycin Solution  5 ml                     

P/S  青霉素/鏈霉素溶液        Penicillin/Streptomycin Solution  100 ml                        

AMS      抗真菌溶液       Antimycotic Solution    50 ml                          

ABAMS       抗菌/抗霉菌溶液      Antibiotic/Antimycotic Solution  50 ml