齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1。我必須運行所有的標準和樣品的重復？

是的，重復的運行，以監(jiān)測測定的精度和提高檢測結果的信心。

2。我必須在一次運行所有的樣品嗎？

不，每個試劑盒采用stripwell微孔板。這允許用戶在不同的時間來分析不同數量的樣品。

3。什么類型的可重復性的結果，得到與分析？

每一個工具都有一個包含一個典型數據的圖表的手冊。在運營商的任何變化，移液和洗滌技術，培養(yǎng)時間、溫度、試劑盒的年齡可以引起變化的結果。每個用戶都應該得到自己的標準曲線。

4。是否有可能儲存的試劑比表示？

在不推薦使用條件下的部件組件的存儲，以保證測試的正確性能。

5。我應該如何儲存我的樣品？

樣品應保存在-20°C或更低的溫度。長期儲存，建議凍結他們在- 70余。

6。我可以修改協議嗎？

BG的ELISA試劑盒進行了優(yōu)化，提供*佳的結果。修改的格式或協議可能會給出錯誤的結果。

7。我可以使用一個不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗證在試劑盒插入的樣品類型。未經檢驗的樣品類型。為進一步信息的聯系技術服務。

8。我的樣本產生的值，在動態(tài)范圍內的測定。我可以使用這些值嗎？

建議只有在標準曲線的范圍內下降的樣本值。標準曲線的范圍內的值一般是非線性的，這可能導致不正確的外推值。產生值高于標準的樣品應為（進一步）稀釋和重復。如果樣品低于該測定的范圍內，該樣品被認為是不可檢測的。

9。我每次都要運行一個空白或零標準嗎？

是的，這些都是必需的計算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測到檢測。當排除特定的檢測問題的來源時，它們也非常有幫助。

10。我能改變我在檢測中使用的樣本量嗎？

不建議你改變卷由于BG工具被設計為*佳的性能在給定的體積

11�？梢允褂貌煌慕M件組件嗎？

每一個組件包含有特定批號的組件，以確保所有的組件都進行優(yōu)化，以及與組件中的所有其他組件。對這些特定的地段進行質量檢驗。這是從來沒有建議使用你自己的組件或組件從其他包或供應商。

12。我的標準曲線看起來很好，但我沒有得到一個信號，我的樣本，當我預期，為什么？

樣品不包含被分析物。一個矩陣的效果可能是掩蓋檢測。確保推薦的稀釋液在試劑盒插入后按規(guī)定進行。如果建議稀釋，請檢查是否正確進行稀釋。過稀釋會導致樣品在標準曲線的范圍內下降。

13。你怎么推薦我洗盤子？

如果您使用的是一個自動平板洗衣機，我們建議定期檢查校準，并將該系統在洗滌前沖洗緩沖液沖洗。手動洗衣機也是一樣的。一個中繼器或洗瓶也可以使用。用戶應小心，以確保所有的內容都是送氣和板拍干的無塵紙。【人螺旋肽(THP)ELISA試劑盒現貨】

14。我需要用一個盤子嗎？

可靠的結果可以得到無板振動，但外徑的一般會低于那些使用平板振動器。

15。為什么我要用波長校正450-570nm之間？

對于酶聯免疫吸附試驗，在雙波長讀的是做正確的光密度貢獻的塑料以及，燈和光學波動。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預期的分析物濃度，我應該找到？

一個給定的分析物的量可能會有所不同，不僅從物種的物種，但也組織和細胞來源。這個信息的*佳來源是目前的文獻，很容易通過互聯網在多個科學數據庫訪問。

18。我的光密度有點高（或較低），比那些在手冊中，與我的試劑盒來了。為什么？

光學密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【人螺旋肽(THP)ELISA試劑盒現貨】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【人螺旋肽(THP)ELISA試劑盒現貨】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【人螺旋肽(THP)ELISA試劑盒現貨】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

929564     QX Pro Marker 14-97 kDa (575 µg)          CNY                             

972422     PyroMark Q96 SNP APOE (96) For detection of two polymorphism in APOE (APOE 112 ａnd APOE 158)          CNY                            

133224     NeXtalStock Sodium ac. pH 4.4 (200)                CNY                             

301425     Effectene Transfection Reagent (1 ml)    1 ml Effectene Reagent, Enhancer, Buffer; for 40 transfections in 60 mm dishes or 160 transfections in 12-well plates  CNY                             

9012693  Probe Tubing Holding Screw with bushing                 CNY                             

9002008  Rotor-Gene Q MDx 2plex, Priority  Rotor-Gene Q MDx 2plex, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

971460     therascreen® KRAS Pyro Kit (24) CE                   CNY                             

292043     mericon Turkey (24)                   CNY                             

133207     NeXtalStock K/Na tartrate (200)               CNY                             

292073     mericon Ruminant Kit (24)                CNY                              【人螺旋肽(THP)ELISA試劑盒現貨】

929104     QIAxcel RNA QC Kit v2.0 (1200)                CNY                             

206042     Type-it Fast SNP Probe PCR Kit (100)        "Features

 Outstanding separation for reliably high call rates

 For difficult SNP loci ａnd for low amounts of template

 Validated using TaqMan SNP Genotyping Assays

 Up to 40% time savings due to fast-cycling procedure

 Convenient ｍaster mix format ａnd optimized protocol"   CNY                             

205314     QuantiTect Reverse Transcription (400)           CNY                             

205313     QuantiTect Rev.Transcription Kit (200)    "For 200 x 20 µl reactions: gDNA Wipeout Buffer, Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, RT Primer Mix, RNase-Free Water.

The contents of this kit are 4 x the contents of the 205311 (NO bulk!).

Suitable only for cDNA synthesis followed by real-time analyses." CNY                             

291055     mericon GMO Screen CTP2-CP4EPSPS (96)               CNY                             

9001918  PyroMark Q96 MD, PrioPLUS  "PyroMark Q96 MD Instrument

Installation of PyroMark Q96 MD instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

9001914  PyroMark Q24, PrioPLUS "PyroMark Q24 Instrument

Installation of PyroMark Q24 & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10

Loaner available in 2 days*

Annual Inspections (1 onsite or RRC  PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)

(In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs)"         CNY                             

9001898  QIAxtractor, UV/HEPA (w/o PC) PrioPLUS        "QIAxtractor  Instrument

Installation of QIAxtractor instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

133112     NeXtalStock Sodium formate (200)          CNY                             

965152     QIAamp DNA Blood BioRobot MDx Kit (12)   For 12 x 96 DNA preps: 12 QIAamp 96 Plates,Buffers,† QIAGEN Protease, ElutionMicrotubes CL, Caps, S-Blocks, Tape Pad      CNY                             

962241     QIAprep 96 Plus BioRobot Kit (4)              CNY                             

51204       FlexiGene DNA Kit (50)    For purification of DNA from 50 ml whole blood: Buffers, QIAGEN Protease CNY                             

9015484  Reagent Holder, 6-bottle, 30 ml               CNY                             

9015474  Cable bare, P             CNY                             

9015472  Motor, Y            CNY                             

960141     QIAwell 96 Ultra BioRobot Kit (4)    For 4 x 96 ultrapure plasmid minipreps, 4 each: QIAfilter 96, QIAwell 96, ａnd QIAprep 96 Plates; Flat-Bottom Blocks ａnd Lids, Reagents, Buffers, Collection Microtubes (1.2 ml) ａnd Caps, 96-Well Microplates RB ａnd Lids, Tape Pads         CNY                              【人螺旋肽(THP)ELISA試劑盒現貨】

955134     EZ1 Virus Mini Kit v2.0 (48)               CNY                             

953034     EZ1 DNA Tissue Kit (48)   48 Reagent Cartridges (Tissue), 50 Disposable Tip Holders, 50 Disposable Filter-Tips, 50 Sample Tubes (2.0 ml), 50 Elution Tubes (1.5 ml), Buffer G2, Proteinase K CNY                             

9018454  Pivot pin, N2, w E-clip, QX                 CNY                             

9015457  Motor, Z/P, M96                CNY                             

9015411  Unit, tip connector, M96          CNY                             

QY-L0189 朝藿定A1140147-77-9   Epimedin A1     140147-77-9    20mg                          

QY-L0190 寶藿苷Ⅰ113558-15-9    Baohuoside I    113558-15-9    20mg                          

QY-L0191 淫羊藿次苷I（95%）    Icarisid I           20mg                          

QY-L0192 脫水淫羊藿素38226-86-7      Anhydroicaritin       38226-86-7       20mg                          

QY-L0193 淫羊藿素737-52-0 Oxypeucedanin         737-52-0  20mg                          

QY-L0194 川陳皮素10236-47-2      Naringin   10236-47-2       20mg                          

QY-L0195 去甲基川陳皮素2174-59-6   Demethylnobiletin   2174-59-6         20mg                          

QY-L0196 刺芒柄花素485-72-3      Formotin  485-72-3  20mg                          

QY-L0197 芒柄花苷486-62-4 Ononin     486-62-4  20mg                          

QY-L0198 川楝素58812-37-6 Toosendanin     58812-37-6       20mg                          

QY-L0199 次野鳶尾黃素41743-73-1      Irisflorentin      41743-73-1       20mg                          

QY-L0200 射干苷611-40-5      Tectoridin         611-40-5  20mg                          

QY-L0201 鳶尾黃素548-77-6 Tectorigenin     548-77-6  20mg                          

QY-L0202 柴胡皂苷A20736-09-8   Saikosaponin A          20736-09-8       20mg                          

QY-L0203 柴胡皂苷B158558-08-0         "Saikosaponin B1

"       "58558-08-0

"       20mg                           【人螺旋肽(THP)ELISA試劑盒現貨】

QY-L0204 柴胡皂苷B258316-41-9 Saikosaponin B2       58316-41-9       20mg                          

QY-L0205 柴胡皂苷C20736-08-7   Saikosaponin C 20736-08-7       20mg                          

QY-L0206 柴胡皂苷D20874-52-6   Saikosaponin D         20874-52-6       20mg                          

QY-L0207 蟾毒靈465-21-4      Bufalin      465-21-4  20mg                          

QY-L0208 蟾毒它靈 471-95-4         Bufotaline         471-95-4         20mg                          

QY-L0209 華蟾酥毒基（97%）470-37-1        Cinobufagin      470-37-1  20mg                          

QY-L0210 華蟾毒它靈 1108-68-5         Cinobufotalin 1108-68-5       20mg                          

QY-L0211 日蟾毒它靈     Gamabufalin             20mg                          

QY-L0212 沙蟾毒精（95%）17008-69-4        Bufarenogin     17008-69-4       20mg                          

QY-L0213 酯蟾毒配基（97%）465-39-4        Resibufogenin  465-39-4  20mg                          

QY-L0214 常春藤皂苷元465-99-6 Hederagenin    465-99-6  20mg                          

QY-L0215 α-常春藤皂苷35790-95-5    A-Hederin         35790-95-5       20mg                          

QY-L0216 蟲草素73-03-0        Cordycepin       73-03-0    20mg                          

QY-L0217 腺苷58-61-7   Adenosine         58-61-7    20mg                          

QY-L0218 重樓皂苷I50773-41-6    Chonglou Saponin I  50773-41-6       20mg                          

QY-L0219 重樓皂苷II76296-72-5   Chonglou Saponin II 76296-72-5       20mg                           【人螺旋肽(THP)ELISA試劑盒現貨】

QY-L0220 薯蕷皂苷19057-60-4      Dioscin     19057-60-4       20mg                          

QY-L0221 重樓皂苷Ⅵ55916-51-3  Polyphyllin VI   55916-51-3       20mg