價格詳細資料：試劑盒價格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認同。齊一生物大品牌保證，傾力為國內(nèi)外科研院校實驗室提供*優(yōu)質(zhì)產(chǎn)品。

【人T細胞活化連接蛋白(LAT)ELISA試劑盒廠家】注意事項

1. 當混合蛋白溶液時應盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時間控制在5分鐘內(nèi)，如標本數(shù)量多，推薦使用排槍加樣。

4. 請每次測定的同時做標準曲線，做復孔。

5. 如標本中待測物質(zhì)含量過高，請先稀釋后再測定，計算時請*后乘以稀釋倍數(shù)。

6. 在配制標準品、檢測溶液工作液時，請以相應的稀釋液配制，不能混淆。

7. 底物請避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人T細胞活化連接蛋白(LAT)ELISA試劑盒廠家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人T細胞活化連接蛋白(LAT)ELISA試劑盒廠家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人T細胞活化連接蛋白(LAT)ELISA試劑盒廠家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9011892       Tubing, teflon, M6, 2.5 x 0.5 mm, 0.65 m              CNY                          

9011887       Holder, tip rack, front unit          CNY                          

9011881       Front unit, BR9600             CNY                          

9011878       Shaker adapter, microplate Adapter for accommodating four 96-well plates or othe labware with the same base dimensions) on the 4-plate shaker system; not for use with the robotic handling system   CNY                          

9011862       Worktable right, BR8000 v1.01        CNY                          

9019147       IPC Hard Drive - no software, PyroQ96        CNY                          

9019072       PyroMark Plate Hotel, PyroQ96MD              CNY                          

9019062       PyroMark Q24 Software    For use with PyroMark Q24 instrument.Software for setting up a run ａnd analyzing mutations ａnd CpGs    CNY                          

9019000       Cover, rear, EZ1xl              CNY                          

9018998       Cover, front, top, EZ1xl             CNY                          

9018956       Reagent Block, 24x2ml Flat-Base Tubes         CNY                          

9018945       Adapter QIAGEN BioRobot M48 Tubes              CNY                          

9022706       Clevis Pin, X-pulley, DML3000        CNY                          

9016062       Tubing Set, Wash pump, BR8000 I          CNY                          

9016031       Tubing, retractable, DISU, BR8 RD v2           CNY                           【人T細胞活化連接蛋白(LAT)ELISA試劑盒廠家】

9015712       DISU Retraction Mechanism BR8,complete           CNY                          

9015654       Communication cable RS485, BR M48          CNY                          

9015594       PM Kit Biorobot EZ1         CNY                          

9019160       Valve - Dispense, PyroQ96MD        CNY                          

9014206       Tubing Set, Waste Sys., BR8 RD             CNY                          

9014139       Sensor; 4l-Bottle; Vac.-Cont.; BR8000           CNY                          

9014118       Tubing, vacuum, textile reinforced, 3m             CNY                          

9014061       Magnet, 96-well, Type A           CNY                          

9021199       Transport Box  PyroMark Q24              CNY                          

9021163       QIAxcel ScreenGel Software            CNY                          

35370    Penta-His Alexa Fluor 647 Conjugate      125 ul Penta-His Alexa Fluor 647 Conjugate, 200 ug/ml       CNY                          

9018300       EZ1 Advanced Test Card          CNY                          

9018293       EZ1 Advanced DNA Blood Card            CNY                          

800354  Setup Large Clone / Large Scale /Genomic            CNY                          

9239544       Assy, XY, Slotted Optical Switch 2          CNY                          

9015287       Filter, dust, left, M48          CNY                           【人T細胞活化連接蛋白(LAT)ELISA試劑盒廠家】

QM101-6     Pfu DNA Polymerase(2.5u/ul)   250U                                

QM101-8     long taq DNA Polymerase(2.5u/ul)    250U                                

QM101-9     冷抑制熱啟動HotStart Taq DNA Polymerase     250U                                

QM101-09   2×HotStart Taq PCR MasterMix（含染料）     0.5ml                                 

QM101-12   10x JumpStart Buffer 熱啟動緩沖液(不含酶）  1ML                                 

QM101-14   2 x SY Green qPCR Mix   50200                               

QM201-1     2×Taq PCR MasterMix(含染料） 1ml5×1ml                               

QM201-3     2×Taq PCR MasterMix（不含染料） 1ml5×1ml                               

QM201-5     2×Taq Plus PCR MasterMix(含染料） 0.5ml5×1ml                            

QM201-7     2×Taq Plus PCR MasterMix（不含染料） 0.5ml5×1ml                             【人T細胞活化連接蛋白(LAT)ELISA試劑盒廠家】

QM201-9     2×Pfu PCR MasterMix(含染料）  0.5ml5×1ml                            

QM201-11   2×Pfu PCR MasterMix（不含染料）  0.5ml5×1ml                            

QM201-13   2×Long Taq PCR Master Mix（含染料）  0.5m                                 

QM201-15   2×Long Taq PCR Master Mix（不含染料）     0.5m                                 

QM201-17   2×HotStart Taq PCR MasterMix（含染料）     0.5m                                 

QM201-19   2×HotStart Taq PCR MasterMix（不含染料） 0.5m                                 

QM301-1     超純 dNTP Mixture(2.5 mM each) 1ML                                 

QM301-4     超純 dNTP Mixture(10 mM each)  1ML                                 

QM301-5     dATP 100mMsolution  0.25 ml                              

QM301-6     dCTP 100mM solution       0.25 ml                              

QM301-7     dGTP 100mM solution       0.25 ml                              

QM301-8     dTTP 100mM solution 0.25 ml                              

QM301-9     PCR enhancer      100μ                                 

QT101-4      TUREscript 1st Strand cDNA Synthesis Kit(鏈反轉(zhuǎn)錄試劑盒）     2050                                 

QT101-1      TUREscript H Minus M-MuLV Reverse Transcriptase  5000U                              

QT101-6      TUREscript One Step RT-PCR Kit（一步法反轉(zhuǎn)錄試劑盒）      2050                                  【人T細胞活化連接蛋白(LAT)ELISA試劑盒廠家】

QT101-8      TUREscript SY Green qRT-PCR Kit （兩步法熒光定量反轉(zhuǎn)錄試劑盒） 2050                                 

QD101-1      DNA MarkerⅠ（條帶100.200.300.400.500.600） "50T

"                                 

QD101-3      DNA Marker Ⅱ（條帶100.300.500.700.900.1200）    "50T

"                                 

QD101-5      DNA Marker Ⅲ（條帶300.500.800.1500.2000.3000.5000）     "50T

"                                 

QD101-7      DNA Marker Ⅳ（條帶500.1000.1500.3000.5000.8000）   "50T

"                                 

QD101-9      DNA Marker Ⅴ（條帶200.400.700.1000.1500.2000）       "50T

"                                 

QD101-11    DNA MarkerⅥ(條帶250.1000.2500.5000.7000.10000)  "50T

"