【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【人小乳腺表皮粘蛋白(SBEM)ELISA試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人小乳腺表皮粘蛋白(SBEM)ELISA試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人小乳腺表皮粘蛋白(SBEM)ELISA試劑盒現(xiàn)貨】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人小乳腺表皮粘蛋白(SBEM)ELISA試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問題請(qǐng)來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9015178  Cooling Block, 48-tube, 0.2 ml, M48           CNY                           

9015175  Reagent Holder Rack, M48           CNY                           

9015036  Probe holder, buffer track, BR8000             CNY                           

9015035  W-Drive, Complete, BR8 RD v2.0        CNY                           

133201    NeXtalStock Imidazole pH 7.0 (200)           CNY                           

929008    QIAxcel DNA Fast Analysis Kit (3000)        CNY                           

929002    QIAxcel DNA High Resolution Kit (1200)            CNY                           

206151    QIAGEN Multiplex PCR Plus Kit (30)          CNY                           

292033    mericon Chicken (24)           CNY                           

51194      QIAamp DNA Blood Maxi Kit (50)       For 50 DNA maxipreps: 50 QIAamp Maxi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (50 ml) CNY                           

133098    NeXtalStock Potassium iodide (200)            CNY                           

133093    NeXtalStock Potassium acetate (200)          CNY                            【人小乳腺表皮粘蛋白(SBEM)ELISA試劑盒現(xiàn)貨】

9015514  PM Kit Biorobot M 96           CNY                           

800381    Database Search            CNY                           

133001    NeXtalStock 1,6-Hexanediol (200)              CNY                           

9243520  QIAlink, Special Integration Service     "QIAlink Special Integration Service includes:

On site software configuration for new instruments ａnd software configurationi for lab developed test. Service may be used also for customer training separately, a refresh training, or a training for new hires. All travel ａnd labor included."      CNY                           

9014406  Upgrade kit, LabHandT-RapidPlate              CNY                           

9014341  Tubing, pump inlet, DISU, BR8000             CNY                           

9019914  PCB, HEPA filter, CAS          CNY                           

9018171  Light/uv, portal, Qsym          CNY                           

9018166  Ventilator, chassis, Qsym              CNY                           

9017852  Rotor Nut              CNY                           

9017840  Tool, calibration tip, QIAC            CNY                           

9017833  Sensor, door, QIAC              CNY                           

9017809  Foot, QIAC           CNY                           

9017807  Drawer, waste, QIAC           CNY                           

9017789  Motor, Y-drive, complete, QIAC           CNY                           

9017727  BracketContact, ProbeHolder, BR8 RD v4.0        CNY                            【人小乳腺表皮粘蛋白(SBEM)ELISA試劑盒現(xiàn)貨】

9015283  Cover, Z, M48              CNY                           

9015274  Laboratory Cabinet BR8000RD            CNY                           

9001909  QIAgility HEPA/UV (w/o PC) Priority   QIAgility HEPA/UV (w/o PC) Priority includes the QIAgility instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.       CNY                           

α-突觸核蛋白, E46K突變體      Alpha-Synuclein, E46K mutant      1.0mg                                

β-突觸核蛋白      Beta-Synuclein       0.5mg                                

β-突觸核蛋白      Beta-Synuclein       1.0mg                                

15N,α-突觸核蛋白      15N, Alpha-Synuclein, Uniform Label   0.5mg                                

15N,α-突觸核蛋白      15N, Alpha-Synuclein, Uniform Label   1.0mg                                

γ-突觸核蛋白      Gamma-Synuclein  0.5mg                                

γ-突觸核蛋白      Gamma-Synuclein  1.0mg                                

α-突觸核蛋白, δ NAC     Alpha-Synuclein, Delta NAC  0.5mg                                

α-突觸核蛋白, 112 (NACP 112)  Alpha-Synuclein, 112 (NACP 112) 0.5mg                                

QPH00121      Recombinant Human beta-NGF     Peprotech       5ug/20ug/                           

QPH00122      Recombinant Murine β-NGF  Peprotech       5ug/20ug/                           

QPH00123      Recombinant Human BMP-2  Peprotech       2ug/10ug/                           

QPH00124      Recombinant Murine EGF     Peprotech       100ug/500ug/                       【人小乳腺表皮粘蛋白(SBEM)ELISA試劑盒現(xiàn)貨】

QPH00125      Recombinant Human EGF     Peprotech       100ug/500ug/                      

QPH00126      Recombinant Human FGF-acidic   Peprotech       50ug/1mg                           

QPH00127      Recombinant Human FGF-acidic   Peprotech       10ug/50ug/                         

QPH00128      Recombinant Murine FGF-acidic   Peprotech       10ug/50ug/                         

QPH00129      Recombinant Human FGF-basic    Peprotech       50ug/1mg                           

QPH00130      Recombinant Human FGF-basic    Peprotech       10ug/50ug/                         

QPH00131      Recombinant Murine FGF-basic    Peprotech       10ug/50ug/                         

QPH00132      Recombinant Murine G-CSF  Peprotech       2ug/10ug/                           

QPH00133      Recombinant Human G-CSF  Peprotech       2ug/10ug/                           

QPH00134      Recombinant Human GM-CSF      Peprotech       10ug/1mg/                          

QPH00135      Recombinant Human GM-CSF      Peprotech       2ug/10ug/                           

QPH00136      Recombinant Murine GM-CSF      Peprotech       10ug/1mg/                          

QPH00137      Recombinant Murine GM-CSF      Peprotech       2ug/10ug/                           

QPH00138      Recombinant Human HGF     Peprotech       2ug/10ug/                           

QPH00139      Recombinant Human IFN-r   Peprotech       20ug/100ug/                       

QPH00140      Recombinant Murine IFN-r   Peprotech       20ug/100ug/                        【人小乳腺表皮粘蛋白(SBEM)ELISA試劑盒現(xiàn)貨】

QPH00141      Recombinant Human IGF-1   Peprotech       100ug/1mg                         

QPH00142      Recombinant Human IGF-1   Peprotech       20ug/100ug/                       

QPH00143      Recombinant Murine IGF-1   Peprotech       10ug/50ug/                         

QPH00144      Recombinant Human KGF     Peprotech       10ug/1mg/