檢測(cè)標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測(cè)方法：ELISA

檢測(cè)類(lèi)型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠(chéng)為您服務(wù)！本試劑盒用于測(cè)定血清,血漿及相關(guān)液體樣本中含量.

【人糖化血紅蛋白A1c(GHbA1c)ELISA試劑盒廠家】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值）,通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

【人糖化血紅蛋白A1c(GHbA1c)ELISA試劑盒廠家】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過(guò)氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人糖化血紅蛋白A1c(GHbA1c)ELISA試劑盒廠家】

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測(cè)樣品孔.在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿(mǎn)洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測(cè)定：以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）. 測(cè)定應(yīng)在加終止液后15分鐘以?xún)?nèi)進(jìn)行.

注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開(kāi)封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果. 【人糖化血紅蛋白A1c(GHbA1c)ELISA試劑盒廠家】

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說(shuō)明書(shū)的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說(shuō)明書(shū)有異,以英文說(shuō)明書(shū)為準(zhǔn).

計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見(jiàn)應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

51504       QIAamp DNA Stool Mini Kit (50)      For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEX tablets, Buffers, Collection Tubes (2 ml)  CNY                             

9002013  Rotor-Gene Q MDx 2plex HRM System            CNY                             

970032     PyroMark Q24 CpG MGMT (4 x 24) For quantification of methylation level in region 17-39 of exon 1 of the MGMT gene.         CNY                             

922920     LiquiChip Filter Microplates (10)               CNY                             

292023     mericon Cattle (24)           CNY                             

292013     mericon Pig Kit (24)          CNY                              【人糖化血紅蛋白A1c(GHbA1c)ELISA試劑盒廠家】

9001903  QIAgility HEPA/UV (PC) Priority        QIAgility HEPA/UV (PC) Priority includes the QIAgility instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                             

133122     NeXtalStock Potassium citrate (200)                 CNY                             

963141     QIAquick 96 PCR BioRobot Kit (4)    For purification of 4 x 96 PCR products: 4 QIAquick 96 Plates, Reagents, Buffers, Collection Microtubes (1.2 ml) ａnd Caps, 96-Well Microplates RB ａnd Lids, Tape Pads  CNY                             

133193     NeXtalStock di-Potassium phosphate (200)               CNY                             

9018555  Cover, front, top, EZ1a              CNY                             

9018528  User Manual EZ1 Advanced              CNY                             

37601       Qproteome Plasma Membrane Protein Kit              CNY                             

9015329  O-rings, Norpren tube fixing, M48 (2)               CNY                             

9015328  Seal, cap, M48 (6)             CNY                             

239095     GelPilot 1 kb Plus Ladder (100)         Ready-to-use DNA marker, 600µl (100lanes), 12 fragments: 100 – 10000 bp         CNY                             

133127     NeXtalStock Sodium citrate (200)             CNY                             

133126     NeXtalStock TRIS-HCl pH 8.5 (200)           CNY                             

133008     NeXtalStock 2-Propanol (200)           CNY                             

133007     NeXtalStock MES sod. salt untit. (200)              CNY                             

9011949  Board, X-main           CNY                             

9011945  Tube Holder, STS, 11ml (6)       Holder for accommodating 8 tubes (11 ml) in the sample tracking system; pack of 6    CNY                              【人糖化血紅蛋白A1c(GHbA1c)ELISA試劑盒廠家】

9243588  RCS, Premium Agreement       "- Service response time RCS: 24 Hrs

- Inspection/maintenance delivery: yes, included; 1 /agreement year

- Cost coverage for repair parts: yes, included

- Cost coverage for labor: yes, included

- Cost coverage for travel: yes, included

- Replacement system provision for DML: yes, included

- Transportation cost coverage for DML: yes, included

For defect units, after customer calling QIAGEN technical service hotline before 9 am:

RCS:

- QIAGEN shall dispatch a specialist to repair the defect instrument at customer site within 24 Hrs.

DML:

- A special package containing a replacement unit shall be sent to the customer within 48 Hrs / 2 working days.

- Customer shall send the defect unit to QIAGEN Regional Repair Center following the instructions attached with the packaging.  

Note: for DML owned by customer, QIAGEN Regional Repair Center will send back the repaired original unit to customers within 7-10 working days.

PC, Monitor, Heater, Shaker, Washer, Printer ａnd Votexer:

- A new unit shall be sent to customer within  7-10 working days at no extra cost

- Customer shall send the defect unit to QIAGEN for disposal

- On-site installation of PC ａnd application software is not covered by the agreement

The Preventive Maintenance (PM) service ensures the correct operation of the equipment. During the PM, all the instrument modules are inspected by a qualified QIAGEN service technician. The Preventive Maintenance is performed under the manufacturer guidelines ａnd documented according to GLP / GMP"    CNY                             

37013       Albumin Affinity Cartridges (3 x 1ml)                 CNY                             

36910       Single-Tube Magnet         Magnet for separating magnetic particles in a 1.5 ml or 2 ml tube         CNY                             

36111       Ni-NTA Magnetic Agarose Beads (2 x 1 ml)     2 x 1 ml nickel-charged magnetic agarose beads (5% suspension)  CNY                             

35330       Penta-His Alexa Fluor 532 Conjugate       125 ul Penta-His Alexa Fluor 532 Conjugate, 200 ug/ml CNY                             

218432     Mouse miScript Assay 384 Set VXX.X (20)                  CNY                             

218412     Mouse miScript Primer Assay Set VXX.X           CNY                             

218380     #miScript PCR Control Set        miScript PCR Control Set allows customers to identify easily the right normalization control for their real-time PCR set up while working with miRNAs or other small RNAs. It includes 6 normalization control assays (for 50 real-time PCR rxns a 50µl) which are all designed against snoRNAs or snRNAs. Works for human mouse ａnd rat.        CNY                             

218193     miScript PCR Starter Kit (80)             CNY                             

QYD115   豬囊蟲(chóng)抗體檢測(cè)試劑盒         96孔/盒 評(píng)估豬場(chǎng)豬瘟疫苗免疫狀況，感染豬的血清學(xué)診斷                                    

QYD116   豬輪狀病毒IgM抗體（RV-IgM)檢測(cè)試劑盒        97孔/盒 用于測(cè)定豬血清、血漿及相關(guān)液體樣本中輪狀病毒IgM 抗體（RV-IgM)表達(dá)                              

QYD117   豬大腸桿菌K99 檢測(cè)試劑盒        96孔/盒 用于測(cè)定豬血清、血漿及相關(guān)液體樣本中大腸桿菌K99表達(dá)                                      【人糖化血紅蛋白A1c(GHbA1c)ELISA試劑盒廠家】

QYD118   豬大腸桿菌987P 檢測(cè)試劑盒     96孔/盒 用于測(cè)定豬血清、血漿及相關(guān)液體樣本中大腸桿菌987P表達(dá)                                   

QYD119   豬大腸桿菌K88 檢測(cè)試劑盒       96孔/盒 用于測(cè)定豬血清、血漿及相關(guān)液體樣本中大腸桿菌K88表達(dá)                                     

QYD120   豬傳染性腸胃炎病毒檢測(cè)試劑盒         96孔/盒 用于測(cè)定豬血清、血漿及相關(guān)液體樣本中傳染性胃腸炎病毒(TGEV)表達(dá)                                    

QYD121   豬流行性腹瀉病毒（PEDV）檢測(cè)試劑盒 96孔/盒 用于測(cè)定豬血清、血漿及相關(guān)液體樣本中流行性腹瀉病毒（PEDV）表達(dá)                                   

QYD122   豬輪狀病毒抗原（RV-Ag)檢測(cè)試劑盒 96孔/盒 用于測(cè)定豬血清、血漿及相關(guān)液體樣本中輪狀病毒抗原（RV-Ag)表達(dá)                                    

QYD123   豬圓環(huán)病毒抗原（PCV）檢測(cè)試劑盒  96孔/盒 用于測(cè)定豬血清、血漿及相關(guān)液體樣本中圓環(huán)病毒（PCV）表達(dá)                             

QYD124   豬鏈球菌（strep）檢測(cè)試劑盒    96孔/盒 用于測(cè)定豬血清、血漿及相關(guān)液體樣本中鏈球菌（strep）表達(dá)                                

QYD125   豬嗜血桿菌(HPS)檢測(cè)試劑盒         96孔/盒 用于測(cè)定豬血清、血漿及相關(guān)液體樣本中嗜血桿菌(HPS)表達(dá)                                    

QYD126   雞禽流感H5N1抗體檢測(cè)試劑盒 96孔/盒                                      

QYD201   雞禽流感H9抗體檢測(cè)試劑盒       96孔/盒                                      

QYD201   雞傳染性喉氣管炎抗體檢測(cè)試劑盒     96孔/盒                                      

QYD201   雞法氏囊抗體檢測(cè)試劑盒     96孔/盒                                      

QYD201   雞新城疫抗體檢測(cè)試劑盒     96孔/盒                                      

QYD301   狗狂犬抗體檢測(cè)試劑盒         96孔/盒                                      

QYD302   旋毛蟲(chóng)抗體檢測(cè)試劑盒         96孔/盒                                      

QYD303   犬惡心絲蟲(chóng)抗體檢測(cè)試劑盒         96孔/盒                                       【人糖化血紅蛋白A1c(GHbA1c)ELISA試劑盒廠家】

QYD401   牛羊亞1型口蹄疫          96孔/盒                                      

QYD402   猴B病毒抗體檢測(cè)試劑盒(定量）        96孔/盒                                      

QYD403   肺吸蟲(chóng)試劑盒         96孔/盒                                      

QYD500   豬藍(lán)耳抗體檢測(cè)卡         50T/盒                                          

QYD501   豬口蹄疫0型抗體檢測(cè)卡     50T/盒                                          

QYD502   豬偽狂犬抗體檢測(cè)卡     50T/盒                                          

QYD503   豬圓環(huán)抗體檢測(cè)卡         50T/盒                                          

QYD504   豬細(xì)小抗體檢測(cè)卡         50T/盒                                          

QYD505   豬乙腦抗體檢測(cè)卡         50T/盒                                          

QYD506   雞禽流感抗體檢測(cè)卡     50T/盒                                          

QYD507   雞禽流感抗原檢測(cè)卡     50T/盒                                          

QYD508   H7亞型禽流感抗原快速檢測(cè)卡   50T/盒                                          

QYD509   H5亞型禽流感抗原快速檢測(cè)卡   50T/盒                                          

QYD510   H9亞型禽流感抗原快速檢測(cè)卡   50T/盒