【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【人糖化蛋白(GSP)ELISA試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人糖化蛋白(GSP)ELISA試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人糖化蛋白(GSP)ELISA試劑盒現(xiàn)貨】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人糖化蛋白(GSP)ELISA試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問題請(qǐng)來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

293035    mericon Apricot kernels (96)        CNY                           

929520    QX Alignment Marker 15bp/500bp (1.5 ml)         CNY                           

9012643  Bottle, system liquid              CNY                            【人糖化蛋白(GSP)ELISA試劑盒現(xiàn)貨】

9012596  Disposable Tips, 300µl (960) Conducting disposable tips    CNY                           

133121    NeXtalStock Lithium citrate  (200)            CNY                           

133119    NeXtalStock Ammonium citrate  (200)              CNY                           

133190    NeXtalStock D-(+)-Glucose (200)              CNY                           

51162      QIAamp 96 DNA Blood Kit (12)    For 12 x 96 DNA preps: 12 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels  CNY                           

50112      QIAamp UCP PurePathogen Blood Kit (10)         CNY                           

133104    NeXtalStock Sodium acet.  (200)              CNY                           

9015468  Sensor, photo, PM-L24         CNY                           

9001871  EZ1 Advanced, System  EZ1 Advanced, System combines the EZ1 Advanced system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                           

9018458  Screw, w finishing washer (4), QX             CNY                           

9018447  Purge Tool, cartridge, QX            CNY                           

9012412  Incubator Tower (65°) for Plato 3 hold        CNY                           

9012392  Bottle, waste, 10 l, BR8000          CNY                           

205111    Omniscript RT Kit (50)  For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water      CNY                           

9018425  Door, sample, QX         CNY                           

9002123  QIAxcel Advanced System    QIAxcel Advanced System combines the QIAxcel Advanced instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                            【人糖化蛋白(GSP)ELISA試劑盒現(xiàn)貨】

57714     QIAamp MinElute Virus Vacuum Kit (50)     For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, Carrier RNA, Buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)     CNY                           

972012    PyroMark Q96 CpG p16 (96)       For quantification of methylation level in p16       CNY                           

133227    NeXtalStock Sodium cacodyl. pH 6.0 (100)        CNY                           

301108    PolyFect Transfection Reagent (100 ml)      For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.  CNY                           

56304     QIAamp DNA Micro Kit (50) For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)  CNY                           

9002031  Rotor-Gene Q MDx 5Plex HRM no PC        CNY                           

9002018  Rotor-Gene Q MDx 2plex HRM, Priority     Rotor-Gene Q 2plex HRM, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents. CNY                           

970902    PyroMark Q24 Advanced Kit (4x24)            CNY                           

133211    NeXtalStock Magnesium chloride H2O (200)             CNY                           

133195    NeXtalStock Sodium tartrate pH 5.6 (200)          CNY                           

293023    mericon Corn (24)         CNY                           

292055    mericon Goat Kit (96)           CNY                           

EK0544   hVEGFR-ELISA     人VEGFR2/KDRELISA試劑盒   Human VEGFR2/KDR ELISA Kit  96 tests                

EK0561   hECad-ELISA 人鈣粘附蛋白ELISA試劑盒       Human E-Cadherin ELISA Kit       96 tests                

EK0563   hCT1-ELISA  人心營(yíng)養(yǎng)素-1ELISA試劑盒 Human Cardiotrophin-1 ELISA Kit 96 tests                

EK0573   hCD40L-ELISA     人可溶性CD40LELISA試劑盒   Human soluble CD40L ELISA Kit  96 tests                

EK0575   hEGVEGF-ELISA  人內(nèi)分泌腺來源的血管內(nèi)皮生長(zhǎng)因子ELISA試劑盒       Human EG-VEGF ELISA Kit 96 tests                

EK0581   hRes-ELISA   人抵抗素ELISAELISA試劑盒    Human Resistin ELISA Kit     96 tests                 【人糖化蛋白(GSP)ELISA試劑盒現(xiàn)貨】

EK0584   hTNF      人TNFβELISA試劑盒       Human TNFβ ELISA Kit     96 tests                

EK0588   hVEGFC-ELISA     人VEGF-CELISA試劑盒     Human VEGF-C ELISA Kit    96 tests                

EK0641   hSurv-ELISA  人生存素ELISA試劑盒       Human Survivin ELISA Kit    96 tests                

EK0642   hTSP2-ELISA 人TSP2ELISA試劑盒  Human TSP2 ELISA Kit 96 tests                

EK0644   hCD105-ELISA      人CD105ELISA試劑盒       Human CD105 ELISA Kit      96 tests                

EK0658   hAPP-ELISA  人APP ELISA試劑盒   Human APP ELISA Kit   96 tests                

EK0663   hBAFF-ELISA       人 BAFF ELISA試劑盒       Human BAFF ELISA Kit 96 tests                

EK0667   hPCad-ELISA 人P-CadherinELISA試劑盒 Human P-Cadherin ELISA Kit       96 tests                

EK0702   hCD40-ELISA       人CD40ELISA試劑盒  Human CD40 ELISA Kit 96 tests                

EK0706   hNCAM-ELISA      人CD56/NCAM-1ELISA試劑盒  Human CD56/NCAM-1 ELISA Kit 96 tests                

EK0744   hcMet-ELISA 人c-Met/HGFRELISA試劑盒      Human c-Met/HGFR ELISA Kit    96 tests                

EK0756   hErbB2-ELISA       人ErbB-2/Neu2ELISA試劑盒      Human ErbB-2/Neu2 ELISA Kit    96 tests                

EK0757   hFeuA-ELISA "人胎球蛋白AELISA試劑盒

"      HumanFetuin-A ELISA Kit     96 tests                

EK0770   hHBEGF-ELISA     人肝素結(jié)合表皮生長(zhǎng)因子ELISA試劑盒   Human HBEGF ELISA Kit     96 tests                

EK0777   hIL6Ra-ELISA       人IL-6RaELISA試劑盒      Human IL-6Ra ELISA Kit      96 tests                

EK0779   hIL7-ELISA    人IL-7ELISA試劑盒   Human IL-7 ELISA Kit   96 tests                 【人糖化蛋白(GSP)ELISA試劑盒現(xiàn)貨】

EK0799   hIL27-ELISA  人IL-27ELISA試劑盒        Human IL-27 ELISA Kit 96 tests                

EK0807   hCSF1R-ELISA     人CSF1R/M-CSFRELISA試劑盒      Human CSF1R/M-CSFR ELISA Kit      96 tests                

EK0816   hKal3-ELISA  人Kallikrein 3ELISA試劑盒 Human Kallikrein 3 ELISA Kit       96 tests                

EK0829   hRANK-ELISA      人 Receptor Activator of NF-κB (RANK)ELISA試劑盒   Human Receptor Activator of NF-κB (RANK) ELISA Kit 96 tests                

EK0850   hMPO-ELISA 人過氧化物酶ELISA試劑盒       Human Myeloperoxidase (MPO) ELISA Kit  96 tests                

EK0859   hPAI1-ELISA  人PAI-1ELISA試劑盒  Human PAI-1 ELISA Kit 96 tests                

EK0867   hDKK1-ELISA       人DKK-1ELISA試劑盒       Human DKK-1 ELISA Kit      96 tests                

EK0873   hPECAM-ELISA    人PECAM-1/CD31ELISA試劑盒       Human PECAM-1/CD31 ELISA Kit      96 tests                

EK0884   hBMP7-ELISA       人BMP-7ELISA試劑盒       Human BMP-7 ELISA Kit      96 tests                

EK0895   hp53-ELISA   人p53 ELISA試劑盒    Human p53 ELISA Kit    96 tests                

EK0904   hCEA-ELISA  人CEAELISA試劑盒    Human CEA ELISA Kit   96 tests