1.購買我公司任意ELISA試劑盒指標(biāo)享受免費(fèi)代測，并提供技術(shù)指導(dǎo)服務(wù)！

二、實(shí)驗(yàn)材料與試劑配制：

1. 儀器與材料：酶標(biāo)儀（使用前預(yù)熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時(shí)備用（如果標(biāo)本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】三. 仔細(xì)閱讀說明書

1.檢查試劑盒標(biāo)簽上的有效期。如果超過有效期，請(qǐng)勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標(biāo)本制備要規(guī)范，每份標(biāo)本體積要按2-3個(gè)復(fù)孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無法實(shí)驗(yàn)者，注意低溫保存

5.準(zhǔn)備好所有實(shí)驗(yàn)額外所需物品，（比如移液器，試管，清洗器，酶標(biāo)儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測標(biāo)本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲(chǔ)。 【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標(biāo)準(zhǔn)品稀釋液或者檢測稀釋液，可能在設(shè)計(jì)時(shí)就含有沉淀物。所有試劑在滴入酶標(biāo)板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標(biāo)板之前，必需不停攪拌。

9.保證充分的孵育時(shí)間和溫度。

10.切勿使用不同生產(chǎn)批號(hào)的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時(shí)，避免泡沫產(chǎn)生。

12.在實(shí)驗(yàn)開始之前，安排好實(shí)驗(yàn)流程。在實(shí)驗(yàn)開始之前，清潔工作臺(tái)。

13.若有問題，應(yīng)與我公司或代理商的技術(shù)支持聯(lián)系

【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測，對(duì)收集后當(dāng)天就進(jìn)行檢測的標(biāo)本，及時(shí)儲(chǔ)存在4℃?zhèn)溆�，如有特殊原因需要周期收集�?biāo)本，請(qǐng)?jiān)炷Ｈ〔暮�，將�?biāo)本及時(shí)分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實(shí)驗(yàn)質(zhì)量，所以避免反復(fù)凍融。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類標(biāo)本需添加抑肽酶。 

2.試劑盒代測標(biāo)本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項(xiàng)請(qǐng)與我司技術(shù)人員溝通。

3.液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

4.購買前請(qǐng)?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測指標(biāo)及其它特殊要求。

【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】五、代測數(shù)據(jù)詳細(xì)內(nèi)容

1、ELISA標(biāo)準(zhǔn)曲線；

2、實(shí)驗(yàn)具體詳細(xì)步驟；

3、完整的實(shí)驗(yàn)報(bào)告（試劑耗材，實(shí)驗(yàn)方法，實(shí)驗(yàn)結(jié)果及結(jié)果說明）；

4、實(shí)驗(yàn)進(jìn)行階段，我公司客服人員會(huì)及時(shí)向您匯報(bào)實(shí)驗(yàn)進(jìn)程。

9020319  PM kit, C12 & QIAgility (UV), CG1             CNY                             

9017625  Motor, y-drive (24V), BR8RD            CNY                             

9020254  LED, LightStrip (white), Qsym           CNY                             

9017354  Holder, VacTrap, BR8                CNY                             

9020201  PCB, lrge breakout, 12 core to rev8, CC2                  CNY                             

9020199  PCB, Z plate breakout,8 ch., 8 core, QX1                  CNY                             

9020193  PCB, vac pump, QXT                  CNY                             

9239523  Fitting,1/16MNPT,1/8, forWasteBottle(5)                  CNY                             

9239536  Assy,Terminated,RedLED,SDS (2)              CNY                             

9239529  Fuse&Holder,1Amp,Fblo,PicoSmd(5)                 CNY                             

9020159  Mask, protection - chamber bottom, RG3                CNY                             

9020145  QIAplex MDD HPV Genotyping LQ Software            CNY                             

9020114  USB cable, QGX                 CNY                             

9020102  Packaging, TLyser2           CNY                              【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】

9020100  Shipping Lock Screw, TLyser 2           CNY                             

9020094  Left Ratchet Holder, TLyser 2           CNY                             

9020090  Motor 24V DC, TLyser 2            CNY                             

9239518  Tubing. Peek, 1/6OD, 010ID, 10 (qty 5)             CNY                             

9239492  Hyd Arm Optic Switch               CNY                             

9239449  3A, 250V Fast Acting Fuse                 CNY                             

9232867  Sensor, pressure, 1600-1mbar                   CNY                             

9236775  Sealing ring, auto vac, SBS top, BR3000           CNY                             

9236509  BR 8000, PM Service        The Overhaul covers all travel ａnd labor costs plus all necessary parts to perform a Preventative Maintenance,. The instrument will also be thoroughly checked ａnd inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.     CNY                             

9239360  Z-Axis Coupling, EZ1                   CNY                             

9236478  BR 9604, Full Agreement         The Full Agreement 9604, 1 PM provides full service coverage for the BioRobot 9604. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).         CNY                             

9236476  #Agreement, 9604, 2PM, 1 yr        An agreement for preventive maintenance provides coverage for travel, labor, ａnd parts, plus the cost of consumables required for two maintenance visits during 1 year. Work carried out during the preventive maintenance visit is fully documented in accordance with GMP/GLP directives. CNY                             

9232726  Block, 2-way, v1.02           CNY                             

9239540  Cable Assy Extender, 2 wire, 22AWG, 12in                CNY                             

9239396  Upgr. LiquiChip Analyzer (RUO)                 CNY                             

9239496  EX XY Motor Driver w/Encoder                 CNY                              【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】

9239494  Sheath Fluid Tubing w/DeBubbler             CNY                             

QY-x638   PC-3前列腺癌細(xì)胞                                                       

QY-x639   BEL-7402肝細(xì)胞癌細(xì)胞                                                       

QY-x640   Tsu-Pr1非雄激素依賴型前列腺癌                                                      

QY-x641   HCC-9204肝癌細(xì)胞                                                      

QY-x642   HOS骨肉瘤細(xì)胞                                                   

QY-x643   Hep3B肝癌細(xì)胞                                                   

QY-x644   MG-63成骨肉瘤細(xì)胞                                                  

QY-x645   Hep G2肝癌細(xì)胞                                                 

QY-x646   Saos-2骨肉瘤細(xì)胞                                               

QY-x647   SMMC-7721肝癌細(xì)胞                                                   【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】

QY-x648   U-2 OS骨肉瘤細(xì)胞                                                       

QY-x649   PAN-1胰腺導(dǎo)管上皮癌細(xì)胞                                               

QY-x650   OS-732骨肉瘤細(xì)胞（瘤株）                                                       

QY-x651   Panc 10.05胰腺腺癌細(xì)胞                                                    

QY-x652   A-204橫紋肌肉瘤細(xì)胞                                                

QY-x653   SW1990胰腺腺癌細(xì)胞                                                

QY-x654   HT1080纖維肉瘤細(xì)胞                                                 

QY-x655   SK-RC-42腎癌細(xì)胞                                               

QY-x656   A375皮膚黑色素瘤細(xì)胞                                                      

QY-x657   RCC-krause腎癌細(xì)胞                                                    

QY-x658   A875黑色素瘤細(xì)胞                                                      

QY-x659   Ketr-3腎癌（瘤株）                                                    

QY-x660   SK-HEL-1皮膚黑色素瘤細(xì)胞                                               

QY-x661   SW 13腎上腺皮質(zhì)瘤細(xì)胞                                                    

QY-x662   A431皮膚基底細(xì)胞癌細(xì)胞                                                 

QY-x663   96-C低轉(zhuǎn)移肺癌細(xì)胞                                                   【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】

QY-x664   CEM白血病細(xì)胞                                                  

QY-x665   95-D高轉(zhuǎn)移肺癌細(xì)胞                                                  

QY-x666   K562慢性髓原白血病細(xì)胞                                                 

QY-x667   973肺腺癌細(xì)胞                                                    

QY-x668   HPB-ALLT細(xì)胞白血病細(xì)胞                                                  

QY-x669   A2肺腺癌細(xì)胞                                                      

QY-x670   HL-60白血病細(xì)胞