【人類(lèi)白細(xì)胞抗原C(HLA-C)ELISA試劑盒現(xiàn)貨】齊一生物供應(yīng)的ELISA試劑盒試驗(yàn)所需自備物品:

1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人類(lèi)白細(xì)胞抗原C(HLA-C)ELISA試劑盒現(xiàn)貨】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買(mǎi)說(shuō)明！

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見(jiàn)的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問(wèn)世以來(lái),得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類(lèi)戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【人類(lèi)白細(xì)胞抗原C(HLA-C)ELISA試劑盒現(xiàn)貨】齊一生物科技?xì)g迎您訂購(gòu)咨詢，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人類(lèi)白細(xì)胞抗原C(HLA-C)ELISA試劑盒現(xiàn)貨】齊一生物供應(yīng)的ELISA試劑盒ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過(guò)的HRP.再經(jīng)過(guò)溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開(kāi)優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過(guò)各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說(shuō)（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開(kāi)放閱讀框2和開(kāi)放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過(guò)氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性. 【人類(lèi)白細(xì)胞抗原C(HLA-C)ELISA試劑盒現(xiàn)貨】

以上資料僅供參考了解，不供于論文寫(xiě)作

齊一生物專(zhuān)業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

9236424  Motor, rotary, TWI           CNY                             

9232970  YZ 96 Connector Board 2 (Arm), E10103                   CNY                             

9236409  Plate Stacker TW I, microplate                  CNY                             

9236357  Sample Holder Large, 1.5ml             CNY                             

9232911  Board, YZ driver                CNY                             

9236336  Analog Board            CNY                             

9236158  Pot. , syr. or vert. , kit, RapidPlate            CNY                             

9236149  Locator Plate, 96 well,pos 4-5,BR RP                CNY                             

9236114  O-Ring, Coupling, RoboVac, BR8 RD v4.2                  CNY                              【人類(lèi)白細(xì)胞抗原C(HLA-C)ELISA試劑盒現(xiàn)貨】

9236107  Flange Tool                CNY                             

9230680  Probe, 0.4 mm, BR8000 v1.01                   CNY                             

9230478  Rack f. Diamend Gel Cards (holds 6cards)                  CNY                             

9230473  Set, Cables, PCB (X-main) to shaker          CNY                             

9235646  Connector, Board, HS shaker, BR8000 v1.0              CNY                             

9235255  T-piece, HSPS tubing, connector               CNY                             

9235190  Motor, clamp, shaker plate,  BR8000             CNY                             

9230060  Rack for reagents (12 x diameter 21mm)                  CNY                             

9238673  Hinge, imbalance system                   CNY                             

9238653  Power Board, temp. switch - heat sink             CNY                             

9238635  Hook identification compl.                CNY                             

9242028  RGQ, Repair Fee      "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: 0

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9241821  Pyro Q96 MDa, Basic Agreement    "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9241786  QIAgility Demopool Box Light Version               CNY                             

9240976  PCB, display, sample tracking, Qsym                 CNY                             

9240968  Barcode camera,  sample tracking, Qsym              CNY                              【人類(lèi)白細(xì)胞抗原C(HLA-C)ELISA試劑盒現(xiàn)貨】

9240816  #Full Agreement, EZ1 DSP, 1YR                 CNY                             

9240680  Spill Tray, Shaker,Modified, AP                   CNY                             

9240790  Update,Waste Bottle Sensor, AP196/98            CNY                             

6000-5014        Lumicheck Plate Service Package, DML2                  CNY                             

9241770  QIAxtractor, Premium Agreement  The Premium Cover Agreement, QIAxtractor provides full service coverage for the QIAxtractor ａnd the CAS 1280. Costs for service visits are fully covered including travel, labor, ａnd parts. One on-site pipetting head service ａnd inspection is included. With a Premium Cover Agreement, there is a priority response time of 24 hours (1 working day).         CNY                             

9238971  Target Prep, Full Agreement   The Full Agreement TargetPrep provides full service coverage for the BioRobot TargetPrep. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days). CNY                             

QY-x1001 人肝癌細(xì)胞Hep3B2.1-7                                                       

QY-x1002 人肝癌細(xì)胞SK-HEP-1                                                   

QY-x1003 人肝癌亞力山大細(xì)胞HCCC-9810                                               

QY-x1004 人膽囊癌細(xì)胞GBC-SD                                                 

QY-x1005 正常人肝細(xì)胞L-02                                                       

QY-x1006 人胚肝細(xì)胞正常QSG-7701                                                 

QY-x1007 人肝細(xì)胞正常HL-7702                                                

QY-x1008 CCL13張氏肝細(xì)胞正常Chang liver                                                      

QY-x1009 人肝星形細(xì)胞正常LX-2                                              

QY-x1010 人肝內(nèi)膽管上皮細(xì)胞HIBEpiC                                                     

QY-x1011 人高轉(zhuǎn)移肝癌細(xì)胞LM3                                                       

QY-x1012 人肝癌細(xì)胞系LM-6                                                      

3QY-x1013        人低轉(zhuǎn)移肝癌細(xì)胞MHCC97-L                                                     

QY-x1014 人肝癌細(xì)胞HepG2/2-15                                              

QY-x1015 人肝癌細(xì)胞9204                                                  【人類(lèi)白細(xì)胞抗原C(HLA-C)ELISA試劑盒現(xiàn)貨】

QY-x1016 人肝癌細(xì)胞HuH-7                                               

QY-x1017 人肝癌細(xì)胞FL62891                                                    

QY-x1018 人膽管細(xì)胞型肝癌細(xì)胞HCCC-9810                                                    

QY-x1019 人肝癌細(xì)胞HCC-9724                                                  

QY-x1020 人肝癌細(xì)胞HHCC                                                

QY-x1021 人肝癌細(xì)胞系Hep-3B                                                 

QY-x1022 表達(dá)HBV病毒的人肝細(xì)胞HepG2.2.15                                                      

QY-x1023 人肝癌細(xì)胞H7402                                              

QY-x1024 人胚肝二倍體細(xì)胞CCC-HEL-1                                                     

QY-x1025 人肝細(xì)胞FL62891                                                

QY-x1026 人感染HCV肝癌細(xì)胞HuH7-HCV                                               

QY-x1027 永生化鼻咽上皮細(xì)胞系NP69-SV40T                                                  

QY-x1028 人鼻咽癌母系細(xì)胞CNE-2Z                                                  

QY-x1029 人鼻咽癌細(xì)胞CNE                                              

QY-x1030 人鼻咽癌細(xì)胞（高分化）CNE1                                                  

QY-x1031 人鼻咽癌細(xì)胞（低分化）CNE2                                                   【人類(lèi)白細(xì)胞抗原C(HLA-C)ELISA試劑盒現(xiàn)貨】

QY-x1032 人鼻咽癌細(xì)胞HNE1                                                     

QY-x1033 人鼻咽癌細(xì)胞HNE2