1.購買我公司任意ELISA試劑盒指標(biāo)享受免費代測，并提供技術(shù)指導(dǎo)服務(wù)！

二、實驗材料與試劑配制：

1. 儀器與材料：酶標(biāo)儀（使用前預(yù)熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時備用（如果標(biāo)本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人非典型性肺炎(SARS-Ab)elisa廠家】三. 仔細(xì)閱讀說明書

1.檢查試劑盒標(biāo)簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標(biāo)本制備要規(guī)范，每份標(biāo)本體積要按2-3個復(fù)孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無法實驗者，注意低溫保存

5.準(zhǔn)備好所有實驗額外所需物品，（比如移液器，試管，清洗器，酶標(biāo)儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測標(biāo)本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲。 【人非典型性肺炎(SARS-Ab)elisa廠家】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標(biāo)準(zhǔn)品稀釋液或者檢測稀釋液，可能在設(shè)計時就含有沉淀物。所有試劑在滴入酶標(biāo)板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標(biāo)板之前，必需不停攪拌。

9.保證充分的孵育時間和溫度。

10.切勿使用不同生產(chǎn)批號的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時，避免泡沫產(chǎn)生。

12.在實驗開始之前，安排好實驗流程。在實驗開始之前，清潔工作臺。

13.若有問題，應(yīng)與我公司或代理商的技術(shù)支持聯(lián)系

【人非典型性肺炎(SARS-Ab)elisa廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標(biāo)本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測，對收集后當(dāng)天就進(jìn)行檢測的標(biāo)本，及時儲存在4℃?zhèn)溆�，如有特殊原因需要周期收集�?biāo)本，請造模取材后，將標(biāo)本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質(zhì)量，所以避免反復(fù)凍融。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類標(biāo)本需添加抑肽酶。 

2.試劑盒代測標(biāo)本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項請與我司技術(shù)人員溝通。

3.液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

4.購買前請?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測指標(biāo)及其它特殊要求。

【人非典型性肺炎(SARS-Ab)elisa廠家】五、代測數(shù)據(jù)詳細(xì)內(nèi)容

1、ELISA標(biāo)準(zhǔn)曲線；

2、實驗具體詳細(xì)步驟；

3、完整的實驗報告（試劑耗材，實驗方法，實驗結(jié)果及結(jié)果說明）；

4、實驗進(jìn)行階段，我公司客服人員會及時向您匯報實驗進(jìn)程。

 QY-04107          乳腺癌易感基因2抗體          Anti-BRCA2（Breast cancer susceptbility gene 2）                                           

 QY-04108          溴脫氧尿苷抗體     Anti-BrdU (Bromodeoxyuridine)                                          

 QY-04109         溴脫氧尿苷單克隆抗體        Anti-BrdU (Bromodeoxyuridine)                                          

 QY-04110          溴脫氧尿苷抗體     Anti-BrdU (Bromodeoxyuridine)                                          

QY-0001   RCA (bovine serum albumin) 牛血清白蛋白            5g/8010g/140100g/1200                                    

QY-0002   KLH (keyhole limpet hemocyanin) 血藍(lán)蛋白           10mg/24050mg/1100100mg/1580                            

QY-0003   OVA (ovalbumin) 雞卵白蛋白                   10mg/10050mg/300100mg/500                                 

QY-0004   THY (thyroglobulin,TG) 甲狀腺球蛋白            10mg/15050mg/650                                   

QY-0005   rHSA人血清白蛋白                   10mg/80100mg/480                                   

QY-00060 Recomb Protein G-HRP酶標(biāo)重組蛋白G                  0.1ml/0                               

QY-04370 Streptavidin,SA鏈霉親和素             1mg/2205mg/88010mg/1600                             【人非典型性肺炎(SARS-Ab)elisa廠家】

QY－－0007  Recomb Protein G/FITC熒光素（FITC）標(biāo)記重組蛋白-G              0.5ml/850                                   

QY-03620 Recomb Protein A重組蛋白A                   1mg/2005mg/80020mg/2500                            

QY-03610 Recomb Protein G重組蛋白G                  1mg/2505mg/1500                            

QY-8280   Cholesterol膽固醇            100g/260500g/10801Kg/1580                                    

QY-01040 β-Amyloid（1-16）β淀粉樣肽1-16（多肽蛋白）              0.5mg/880                                  

QY-08710 Aβ31-35（β-Amyloid 31-35）β-淀粉樣肽31-35                 0.1mg/3200.2mg/480                                 

QY-01050 β-Amyloid（1-28）β淀粉樣肽1-28（多肽蛋白）              0.5mg/880                                  

QY-01070 β-Amyloid fragment（1-42）Ratβ淀粉樣肽（1-42）大鼠                 0.1mg/6000.5mg/2200                              

QY-01060 β-Amyloid（1-40)β淀粉樣肽1-40（多肽蛋白）                 0.1mg/5000.5mg/20001mg/3100                              

QY-01070 β-Amyloid fragment（1-42）β淀粉樣肽（1-42）（抗原）                 0.1mg/6000.5mg/2200                              

QY-00440 β-Amyloid 25-35 Aβ 25-35β-淀粉樣肽25-35             1mg/4005mg/150050mg/15000                                

QY-00760 β-Amyloid 1-42 CTβ淀粉樣肽 1-42 (C端多肽)             0.1mg/5000.5mg/20001mg/3100                              

QY-00570 Amylin糖尿病相關(guān)肽（胰島淀粉樣肽）               0.5mg/6501mg/1100                                  

QY-01120 APP695-770/APP17肽 （Amyloid protein precursor）淀粉樣肽前體蛋白（17肽抗原）                   0.5mg/6501mg/1100                                    

QY-04990 Neuroprotective peptide/Gly-HN(Nuclear-encoded Humanin CAS:HN(N) Gly14)神經(jīng)保護(hù)肽                  0.5mg/1100                                

QY-0158   Amylin(1-37)糖尿病相關(guān)肽（胰島淀粉樣肽）               0.1mg/6500.5mg/2480                               【人非典型性肺炎(SARS-Ab)elisa廠家】

QY-10530 CCP peptide (cyclic citrullinated peptide,CCP)瓜氨酸環(huán)肽                0.5mg/11001mg/1800                               

QY-06160 CCP peptide (cyclic citrullinated peptide,CCP)瓜氨酸環(huán)肽                0.5mg/1100                               

QY-00380 GLP-1（Glucagon-like peptide-1）(7-36)胰高血糖素樣肽-1（多肽）                    0.1mg/5000.5mg/2000                              

QY-0108   GRGDS peptide抗黏附多肽GRGDS                 1mg/3005mg/1200                            

9001658  Rotor-Gene Q 5plex HRM (w/o PC) Priority     Rotor-Gene Q 5plex HRM (w/o PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

951054     EZ1 DNA Blood 350 µl Kit (48)          "

48 Reagent Cartridges (Blood 350 ul), 50 Disposable Tip Holders, 50 Disposable Filter-Tips, 50 Sample Tubes (2.0 ml), 50 Elution Tubes (1.5 ml)"  CNY                             

9001874  EZ1 Advanced XL, System         EZ1 Advanced XL, System combines the EZ1 Advanced XL instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.          CNY                             

9015466  Cover, P             CNY                             

9012413  Z-Bars Gripper, RCS1                 CNY                             

9012410  Tischplatte Plato 3301S Technikk  (9194)                CNY                             

205110     Omniscript RT Kit (10)      For 10 reverse-transcription reactions: 40 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water         CNY                             

204845     QuantiTect Multiplex RT-PCR NR Kit(1000)               CNY                             

133178     NeXtalStock Citric acid pH 2.6 (200)                  CNY                             

133165     NeXtalStock CAPS pH 11.4 (200)               CNY                             

9018409  Regulator, N2, internal, front, QX            CNY                             

9015406  Driver, motor, M96           CNY                             

9001861  Rotor-Gene Q 2plex (PC) Priority     Rotor-Gene Q 2plex (PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

9012327  Peristaltic Pump Outlet Tubing                  CNY                              【人非典型性肺炎(SARS-Ab)elisa廠家】

1067925  Buffer AW1 (175 ml)                  CNY                             

38045       GNA Cartridge (5ml)                  CNY                             

38003       ConA Cartridges (3 x 1ml)                  CNY                             

9015365  Drop catcher, M96           CNY                             

204741     QuantiTect Multiplex PCR NoROX Kit (40)       "Trial kit size for 40 x 50 ul reactions: 1.7 ml QuantiTect Multiplex PCR NoROX Master Mix, 2.0 ml RNase-free water

For quantitative, real-time PCR ａnd two-step RT-PCR using probe detection with multiple assays."     CNY                             

133066     NeXtalStock Manganese(II) chloride (200)                CNY                             

290065     mericon Cronobacter spp Kit (96)             CNY                             

204374     Rotor-Gene Probe PCR Kit (400)      The Rotor-Gene Probe PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly sensitive quantification in real-time PCR ａnd two-step RT-PCR using sequence-specific probes.  CNY                             

9015354  Springs, gas (2)                   CNY                             

133054     NeXtalStock Imidazole HCl (200)               CNY                             

9001671  PyroMark Q24 Instrument       For methylation ａnd mutation analysis using Pyrosequencing technology in 24 well      CNY                             

950901     Capture Plate, standard yield (5)              CNY                             

37430       PhosphoSerine Antibody Q5     100 ug mixture of anti-phosphoserine antibodies(isotypes mouse IgG1 ａnd IgM, for 200 ml working solution)     CNY                             

133030     NeXtalStock Cesium sulfate (200)             CNY                             

9001627  Rotor-Gene Q 2plex (w/o PC) System       Rotor-Gene Q 2plex (w/o PC) System Priority includes the Rotor-Gene system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the Rotorgene-Q systems hardware ａnd trains the user on operation of the Rotorgene software. All travel ａnd labor cost included for Installation ａnd service documented on a GMP/GLP-compliant Field Service document.  CNY                             

9001620  Rotor-Gene Q 2plex System              CNY                              【人非典型性肺炎(SARS-Ab)elisa廠家】

9001603  Rotor-Disc 100 Starter Kit (230V)             CNY                             

9015316  Belt, Y-motor, v2, M48              CNY                             

9015312  Unit, spotlight, right, M48                CNY