齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

1。我必須運(yùn)行所有的標(biāo)準(zhǔn)和樣品的重復(fù)？

是的，重復(fù)的運(yùn)行，以監(jiān)測(cè)測(cè)定的精度和提高檢測(cè)結(jié)果的信心。

2。我必須在一次運(yùn)行所有的樣品嗎？

不，每個(gè)試劑盒采用stripwell微孔板。這允許用戶(hù)在不同的時(shí)間來(lái)分析不同數(shù)量的樣品。

3。什么類(lèi)型的可重復(fù)性的結(jié)果，得到與分析？

每一個(gè)工具都有一個(gè)包含一個(gè)典型數(shù)據(jù)的圖表的手冊(cè)。在運(yùn)營(yíng)商的任何變化，移液和洗滌技術(shù)，培養(yǎng)時(shí)間、溫度、試劑盒的年齡可以引起變化的結(jié)果。每個(gè)用戶(hù)都應(yīng)該得到自己的標(biāo)準(zhǔn)曲線(xiàn)。

4。是否有可能儲(chǔ)存的試劑比表示？

在不推薦使用條件下的部件組件的存儲(chǔ)，以保證測(cè)試的正確性能。

5。我應(yīng)該如何儲(chǔ)存我的樣品？

樣品應(yīng)保存在-20°C或更低的溫度。長(zhǎng)期儲(chǔ)存，建議凍結(jié)他們?cè)?/span>- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進(jìn)行了優(yōu)化，提供*佳的結(jié)果。修改的格式或協(xié)議可能會(huì)給出錯(cuò)誤的結(jié)果。

7。我可以使用一個(gè)不建議在試劑盒插入的樣本類(lèi)型嗎？

該試劑盒已被驗(yàn)證在試劑盒插入的樣品類(lèi)型。未經(jīng)檢驗(yàn)的樣品類(lèi)型。為進(jìn)一步信息的聯(lián)系技術(shù)服務(wù)。

8。我的樣本產(chǎn)生的值，在動(dòng)態(tài)范圍內(nèi)的測(cè)定。我可以使用這些值嗎？

建議只有在標(biāo)準(zhǔn)曲線(xiàn)的范圍內(nèi)下降的樣本值。標(biāo)準(zhǔn)曲線(xiàn)的范圍內(nèi)的值一般是非線(xiàn)性的，這可能導(dǎo)致不正確的外推值。產(chǎn)生值高于標(biāo)準(zhǔn)的樣品應(yīng)為（進(jìn)一步）稀釋和重復(fù)。如果樣品低于該測(cè)定的范圍內(nèi)，該樣品被認(rèn)為是不可檢測(cè)的。

9。我每次都要運(yùn)行一個(gè)空白或零標(biāo)準(zhǔn)嗎？

是的，這些都是必需的計(jì)算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測(cè)到檢測(cè)。當(dāng)排除特定的檢測(cè)問(wèn)題的來(lái)源時(shí)，它們也非常有幫助。

10。我能改變我在檢測(cè)中使用的樣本量嗎？

不建議你改變卷由于BG工具被設(shè)計(jì)為*佳的性能在給定的體積

11�？梢允褂貌煌慕M件組件嗎？

每一個(gè)組件包含有特定批號(hào)的組件，以確保所有的組件都進(jìn)行優(yōu)化，以及與組件中的所有其他組件。對(duì)這些特定的地段進(jìn)行質(zhì)量檢驗(yàn)。這是從來(lái)沒(méi)有建議使用你自己的組件或組件從其他包或供應(yīng)商。

12。我的標(biāo)準(zhǔn)曲線(xiàn)看起來(lái)很好，但我沒(méi)有得到一個(gè)信號(hào)，我的樣本，當(dāng)我預(yù)期，為什么？

樣品不包含被分析物。一個(gè)矩陣的效果可能是掩蓋檢測(cè)。確保推薦的稀釋液在試劑盒插入后按規(guī)定進(jìn)行。如果建議稀釋?zhuān)?qǐng)檢查是否正確進(jìn)行稀釋。過(guò)稀釋會(huì)導(dǎo)致樣品在標(biāo)準(zhǔn)曲線(xiàn)的范圍內(nèi)下降。

13。你怎么推薦我洗盤(pán)子？

如果您使用的是一個(gè)自動(dòng)平板洗衣機(jī)，我們建議定期檢查校準(zhǔn)，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動(dòng)洗衣機(jī)也是一樣的。一個(gè)中繼器或洗瓶也可以使用。用戶(hù)應(yīng)小心，以確保所有的內(nèi)容都是送氣和板拍干的無(wú)塵紙。【人孕酮(PROG)ELISA現(xiàn)貨】

14。我需要用一個(gè)盤(pán)子嗎？

可靠的結(jié)果可以得到無(wú)板振動(dòng)，但外徑的一般會(huì)低于那些使用平板振動(dòng)器。

15。為什么我要用波長(zhǎng)校正450-570nm之間？

對(duì)于酶聯(lián)免疫吸附試驗(yàn)，在雙波長(zhǎng)讀的是做正確的光密度貢獻(xiàn)的塑料以及，燈和光學(xué)波動(dòng)。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過(guò)程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶(hù)決定。

17。什么是預(yù)期的分析物濃度，我應(yīng)該找到？

一個(gè)給定的分析物的量可能會(huì)有所不同，不僅從物種的物種，但也組織和細(xì)胞來(lái)源。這個(gè)信息的*佳來(lái)源是目前的文獻(xiàn)，很容易通過(guò)互聯(lián)網(wǎng)在多個(gè)科學(xué)數(shù)據(jù)庫(kù)訪問(wèn)。

18。我的光密度有點(diǎn)高（或較低），比那些在手冊(cè)中，與我的試劑盒來(lái)了。為什么？

光學(xué)密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【人孕酮(PROG)ELISA現(xiàn)貨】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【人孕酮(PROG)ELISA現(xiàn)貨】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【人孕酮(PROG)ELISA現(xiàn)貨】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

 QY-04076          蛇毒巴曲酶抗體     Anti-Batroxobin                                           

 QY-04077          Bax（小鼠來(lái)源抗體）  Anti-Bax （Bcl-2 Assaciated X protein）                                           

 QY-04078          Bax抗體           Anti-Bax（Bcl-2 Assaciated X protein）                                              

 QY-04079          Bcl-2抗體        Anti-Bcl-2(B cell lymphoma/leukemia-2)                                             

 QY-04080          Bcl-xL蛋白抗體       Anti-Bcl-xL                                           

 QY-04081         p53正向細(xì)胞凋亡調(diào)控因子抗體         Anti-BBC3/PUMA(bcl-2 binding component 3)                                             

 QY-04082          抗磷酸化Bcl-xL(pSer62)蛋白抗體          Anti-phospho-Bcl-xL(pSer62)                                       

 QY-04083          丁酰膽堿脂酶抗體(N端)       Anti-BCHE(butyrylcholinesterase)NT                                           

 QY-04084          抗丁酰膽堿脂酶單克隆抗體         Anti-BCHE(mouse Anti-butyrylcholinesterase Monoclonal)                                        

 QY-04085          丁酰膽堿脂酶抗體(C端)        Anti-BCHE(butyrylcholinesterase)CT                                            【人孕酮(PROG)ELISA現(xiàn)貨】

 QY-04086          腦源神經(jīng)營(yíng)養(yǎng)因子/腦衍化神經(jīng)營(yíng)養(yǎng)因子   Anti-BDNF (Brain-derived Neurotrophin factor)                                         

 QY-04087          一種抑癌基因抗體         Anti-BECN1(Beclin 1)                                           

 QY-04088          β-酪蛋白(抗體)兔抗山羊、綿羊          Anti-Beta-casein(β-casein)                                        

 QY-04089         TNF家族B細(xì)胞激活因子抗體    Anti-BAFF/CD257(B cell activating factor)                                          

 QY-04090          堿性成纖維細(xì)胞生長(zhǎng)因子抗體     Anti-BFGF/FGF-2                                          

 QY-04091          骨保護(hù)蛋白/骨鈣素抗體        Anti-BGP(Osteocalcin/Bone Gla-protein)                                            

 QY-04092          BH3結(jié)構(gòu)域凋亡誘導(dǎo)蛋白抗體     Anti-Bid (BH3 interacting domain death agonist)                                       

 QY-04093         橋連整合因子蛋白抗體        Anti-BIN1(Bridging integrator protein-1)                                            

 QY-04094          細(xì)胞死亡調(diào)解子抗體     Anti-Bim(Bcl-2 interacting mediator of cell death)                                              

 QY-04095          細(xì)胞凋亡抑制因子5抗體     Anti-BIRC5/Survivin variant                                         

 QY-04096          BK通道蛋白抗體   Anti-BK channel (stretch-activated Kca channel)                                        

 QY-04097          鈣激活鉀通道蛋白抗體         Anti-BKCa channels（calcium-activated potassium channel）                                           

 QY-04098         骨形態(tài)發(fā)生蛋白-2抗體        Anti-BMP2 (bone morphogenetic protein 2)                                      

 QY-04099          骨形態(tài)發(fā)生蛋白4抗體          Anti-BMP4（bone morphogenetic protein 4）                                          

 QY-04100         骨形態(tài)發(fā)生蛋白-7抗體        Anti-BMP-7(bone morphogenetic protein-7)                                      

 QY-04101          骨成型蛋白受體1A抗體       Anti-BMPR-1A(Bone Morphogenetic protein Receptor-1A)                                        【人孕酮(PROG)ELISA現(xiàn)貨】

 QY-04102          腦鈉素/利鈉肽抗體      Anti-BNP （Brain Natriuretic Peptide ）                                         

 QY-04103         B細(xì)胞特異性轉(zhuǎn)錄因子抗體         Anti-BOb-1(B-cell oct-binding protein 1)                                              

 QY-04104          B Raf抗體        Anti-B Raf                                            

 QY-04105          乳腺癌易感基因1抗體(人)  Anti-BRCA1（Breast cancer susceptbility gene 1）(human)                                     

 QY-04106          乳腺癌易感基因1抗體（小鼠 大鼠）       Anti-BRCA1（Breast cancer susceptbility gene 1）（mouse rat）                                             

292015     mericon Pig Kit (96)          CNY                             

133123     NeXtalStock Tris (200)               CNY                             

965672     QIAamp One-For-All Nucleic Acid Kit (12)                  CNY                             

133192     NeXtalStock Ammonium tartrate pH 7 (200)           CNY                             

133183     NeXtalStock Citric acid pH 3.5 (200)                  CNY                             

9018513  Adapter, USB-serial, EZ1a                 CNY                             

9001896  QIAxtractor, UV/HEPA (w/o PC) System  QIAxtractor, UV/HEPA (w/o PC) System combines the QIAxtractor system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document. CNY                             

51104       QIAamp DNA Blood Mini Kit (50)     For 50 DNA minipreps: 50 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)        CNY                             

133099     NeXtalStock Potassium nitrate (200)                 CNY                             

133092     NeXtalStock PEG 8,000 (200)            CNY                             

9015516  Heat unit, complete                  CNY                             

9018452  Piston, purge (1), QX                  CNY                             

9012416  Microplate Station 8000, short        Station for accommodating a 96-well plate (or other labware with the same base dimensions) on the BioRobot 8000 or MDx worktable    CNY                             

133168     NeXtalStock CAPSO pH 9.2 (200)               CNY                             

9018424  Door, N2, QX             CNY                              【人孕酮(PROG)ELISA現(xiàn)貨】

9015405  Board, PDB, M96              CNY                             

9015386  Manual, Service, BIOROBOT MDx            CNY                             

9001863  Rotor-Gene Q 2plex HRM (PC) Priority    Rotor-Gene Q 2plex HRM (PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

9001720  Rotor-Gene Q 6Plex no PC                 CNY                             

9012331  Set, Tubing, Pipetting, Plato3xx1              CNY                             

133084     NeXtalStock PEG 300 (200)               CNY                             

38065       #PNA Cartridge (5ml)                CNY                             

204752     QuantiFast Multiplex PCR +R Kit (80)                CNY                             

290095     mericon Quant. L. pneumophila Kit (96)          CNY                             

800423     Library, Large Insert, Picked Clones                   CNY                             

290045     mericon Campylobacter triple Kit (96)              CNY                             

9015353  Controller, temp.               CNY                             

133051     NeXtalStock HEPES sod. salt untit. (200)          CNY                             

9018388  QIAcube Tip Adapter Ring Tool                 CNY                             

9018377  Teaching Tools, Alignment, AP                   CNY                             

9012226  Power Supply, 6 amp for Plato size 90               CNY                              【人孕酮(PROG)ELISA現(xiàn)貨】

9012066  Tray, buffer bottles, roter, BR8000           CNY                             

9001674  PyroMark Q96 MD Automated Instrument     For performing automated pyrosequncing reaction on the Q96 MD      CNY