檢測標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價格公道,傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠為您服務(wù)！本試劑盒用于測定血清,血漿及相關(guān)液體樣本中含量.

【人毒性休克綜合征毒素1(TSST-1)ELISA試劑盒現(xiàn)貨】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長下測定吸光度（OD值）,通過標(biāo)準(zhǔn)曲線計算樣品中該產(chǎn)品濃度.

試劑盒組成：

【人毒性休克綜合征毒素1(TSST-1)ELISA試劑盒現(xiàn)貨】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測樣品孔.在酶標(biāo)包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時藍(lán)色立轉(zhuǎn)黃色）.

11. 測定：以空白空調(diào)零,450nm波長依序測量各孔的吸光度（OD值）. 測定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

【人毒性休克綜合征毒素1(TSST-1)ELISA試劑盒現(xiàn)貨】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請每次測定的同時做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計算時請*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請避光保存.

7． 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號組分不得混用.

10. 如與英文說明書有異,以英文說明書為準(zhǔn).

【人毒性休克綜合征毒素1(TSST-1)ELISA試劑盒現(xiàn)貨】計算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個月

QY-14652 Anti-IGF-II（ Insulin-like Growth Factor-II ）胰島素樣生長因子-II抗體                                                 

QY-14662 IGF-IIBP3（Insulinlike growth factor 2 mRNA binding protein 3）胰島素樣生長因子2 mRNA 結(jié)合蛋白3抗體                                                  

QY-14672 Anti-IKB β (Inhibitor of K B)KB抑制蛋白抗體                                                  

QY-14682 Anti-IKB α (Inhibitor of KBα)KB抑制蛋白α抗體                                                   

QY-14692 Anti-IKI3 family protein擬南芥IKI3抗體                                                     

QY-14702 Anti-IL-10(Interleukin-10)白介素10抗體                                                    

QY-14712 anti-IL-12(Interleukin-12)白介素12抗體（白細(xì)胞介素-12）                                                   

QY-14722 Anti-IL-13(Interleukin-13)白介素13抗體                                                    

QY-14732 anti-IL-16/LCF(interleukin-16)(Lymphocyte Chemotactic Factor)白細(xì)胞介素-16抗體                                                

QY-14742 Anti-IL-17（Interleukin-17）白介素-17抗體                                                       

QY-14752 Anti-IL-18/IGIF(Interleukin-18)白細(xì)胞介素-18/干擾素γ誘導(dǎo)因子抗體                                                     

QY-14762 Anti-IL1RAPL1 （Interleukin 1 receptor accessory protein-like 1）白介素受體相關(guān)蛋白樣1前體蛋白抗體                                                

QY-14772 Anti-IL-1α (Interleukin-1α )白介素1α抗體                                                   

QY-14782 Anti-IL-1β(Interleukin-1β)白介素1β抗體                                               【人毒性休克綜合征毒素1(TSST-1)ELISA試劑盒現(xiàn)貨】

QY-14792 Anti-IL-2 (Interleukin-2)白介素2抗體                                                 

QY-14802 Anti-IL-2(Mouse to Humen Interleukin-2)鼠抗人白細(xì)胞介素-2抗體（單抗）                                                 

QY-14812 Anti-IL-23(Interleukin-23)白介素-23抗體                                                    

QY-14822 Anti-IL-4(Interleukin-4)白介素4抗體                                                  

QY-14832 Anti-IL-6 (Interleukin-6) Human白介素6                                                     

QY-14842 Anti-IL-6 (Interleukin-6) mouse、rat白介素6抗體                                                    

QY-14852 Anti-IL-6(Interleukin-6/interferon, beta 2)mouse rat白介素6抗體                                                    

QY-14862 Anti-IL-8（Interleukin-8）rat mouse白介素8抗體                                                   

QY-14872 Anti-ILK-1 (Integrin-linked protein kinase 1)整合素連接激酶-1抗體                                                  

QY-14882 anti-ING1/p33(inhibitor of growth gene 1)生長抑制因子基因1抗體                                                

QY-14892 Anti-Inhibin α抑制素α抗體                                                      

QY-14902 Anti-Insulin胰島素抗體                                               

QY-14912 Anti-Insulin Receptor-α（ISR-α）胰島素受體-α抗體                                                    

QY-14922 Anti-Insulin(1-51 protein)胰島素1-51蛋白抗體                                                 

QY-14932 Anti-Integrin α3β1整合素α3β1抗體                                                  

QY-14942 Anti-Integrin α4β7/LPAM-1 (Lymphocyte Peyer’s patch Adhesion Molecule 1/Integrin alpha4,beta7)整合素α4β7抗體                                                        【人毒性休克綜合征毒素1(TSST-1)ELISA試劑盒現(xiàn)貨】

QY-14952 anti-Integrin avb3整合素avb3抗體                                                   

19587       Extension Tubes (3ml)                CNY                             

69989       Stainless Steel Beads 5 mm (200)    Stainless Steel Beads, suitable for use with the TissueLyser system         CNY                            

69965       TissueLyser Single-Bead Dispenser, 5mm                  CNY                             

19101       RNase A (17500 U)  2.5 ml (100 mg/ml; 7000 units/ml, solution)   CNY                             

19053       Buffer P3 500 ml Neutralization Buffer   CNY                             

19015       QIArack    1 rack for 12 x QIAGEN-tip 20, 8 x QIAGEN-tip 100, 6 x QIAGEN-tip 500 or HiSpeed Midi Tips, 4 x QIAGEN-tip 2500 or HiSpeed Maxi Tips, or 10 x QIAfilter Midi or Maxi Cartridges     CNY                             

12965       QIAGEN Plasmid Plus Maxi Kit (100)        QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction.      CNY                             

12262       QIAfilter Plasmid Maxi Kit (10)         10 QIAGEN-tip 500, Reagents, Buffers, 10 QIAfilter Maxi Cartridges     CNY                            

12183       QIAGEN Plasmid Mega Kit (25)        25 QIAGEN-tip 2500, Reagents, Buffers CNY                             

133278     NeXtalStock SPG pH 4.0 (200)          CNY                             

4502265  artus VZV RG PCR Kit (96) CE            CNY                              【人毒性休克綜合征毒素1(TSST-1)ELISA試劑盒現(xiàn)貨】

133263     NeXtalStock PCB pH 4.0 (200)          CNY                             

990600     Lysis Plate, 96-well (5)               CNY                             

990554     Reagent Trough (with Lid), 70 mL (10)    ccc   CNY                             

4502063  artus VZV LC PCR Kit (24) CE             CNY                             

949012     Autopure Proteinase K Solution (3500 µl)                  CNY                             

990395     Starter Pack, QIAcube               CNY                             

336312     SureSilencing shRNA Plasmid, Hygromycin               CNY                             

336311     SureSilencing shRNA Plasmid, GFP            CNY                             

990390     Reagent Bottle Rack                 CNY                             

941517     BioSprint 15 DNA Plant Kit (360)               CNY                             

330629     RT² SYBR Green ROX FAST Mastermix (25ml)          CNY                             

1070210  Snap-on connector (1)               CNY                             

979203     PyroMark Control Oligo   For installation check of PyroMark instrument.       CNY                             

9012986  Thermostate system,complete,(220V), 9604           CNY                             

9012937  RCS - Reagent Trough                CNY                             

9012805  Tube Holder, STS, conical, 2ml (6)             CNY                              【人毒性休克綜合征毒素1(TSST-1)ELISA試劑盒現(xiàn)貨】

330610     RT² SYBR Green Fluor FAST Mastermix (2)                CNY                             

330519     RT² SYBR Green Fluor qPCR Mastermix 25ml           CNY                             

929704     QX Colored 0.2 ml 12-Tube Strip (80)                 CNY                             

330509     RT² SYBR Green qPCR Mastermix (25 ml)                 CNY                             

336391     SureSilencing shRNA Control             CNY                             

336161     Multi-Analyte  ELISArray Kit           CNY                             

133146     NeXtalStock ADA pH 7.0 (200)                   CNY                             

133017     NeXtalStock Ammonium iodide (200)                CNY                             

1043367  QIAamp MinElute Virus Accessory Set              CNY                             

950154     Wash Reagent (DXW) (650 ml)                  CNY                             

219000     miScript Target Protector                  CNY                             

218411     Human miScript Primer Assay Set VXX.X          CNY                              【人維生素D受體(VDR)ELISA試劑盒廠家】

218200     Custom miScript Primer Assay (100)                  CNY                             

27115       QIAprep Spin Miniprep Columns (100)              CNY                             

74034       RNeasy Plus Micro Kit (50)                CNY                             

73404       RNeasy Plus Universal Tissue Mini Kit50          CNY                             

20912       Buffer QX1 Indikator (500 ml) 500 ml Solubilization ａnd Binding Buffer (with pH indicator)  CNY