檢測(cè)標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測(cè)方法：ELISA

檢測(cè)類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠(chéng)為您服務(wù)！本試劑盒用于測(cè)定血清,血漿及相關(guān)液體樣本中含量.

【人細(xì)胞毒素(CTX)ELISA試劑盒現(xiàn)貨】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值）,通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

【人細(xì)胞毒素(CTX)ELISA試劑盒現(xiàn)貨】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過(guò)氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測(cè)樣品孔.在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測(cè)定：以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）. 測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

【人細(xì)胞毒素(CTX)ELISA試劑盒現(xiàn)貨】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說(shuō)明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說(shuō)明書有異,以英文說(shuō)明書為準(zhǔn).

【人細(xì)胞毒素(CTX)ELISA試劑盒現(xiàn)貨】計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

QT-00121 地衣芽孢桿菌ATCC11946      凍干粉                                         

QT-00122 谷氨酸棒桿菌ATCC13032      凍干粉                                         

QT-00123 熏衣草灰鏈霉菌ATCC13306 凍干粉                                         

QT-00124 豬霍亂沙門氏菌豬霍亂亞種ATCC13312      凍干粉                                         

QT-00125 脫氮假單胞菌ATCC13867      凍干粉                                         

QT-00126 耐放射異常球菌ATCC13939 凍干粉                                         

QT-00127 綠針假單胞菌ATCC13985      凍干粉                                         

QT-00128 腸炎沙門氏菌腸炎亞種ATCC14028      凍干粉                                         

QT-00129 巨大芽孢桿菌ATCC14581      凍干粉                                         

QT-00130 吸水鏈霉菌奧薩霉素亞種ATCC15420 凍干粉                                         

QT-00131 長(zhǎng)雙歧桿菌ATCC15707 凍干粉                                         

QT-00132 放射性根瘤菌ATCC15955      凍干粉                                          【人細(xì)胞毒素(CTX)ELISA試劑盒現(xiàn)貨】

QT-00133 短密青霉ATCC16024      凍干粉                                         

QT-00134 副溶血性弧菌ATCC17802      凍干粉                                         

QT-00135 單核增生李斯特菌ATCC19115      凍干粉                                         

QT-00136 缺陷短波單胞菌ATCC19146 凍干粉                                         

QT-00137 師崗鏈霉菌ATCC19166 凍干粉                                         

QT-00138 脫氮付球菌ATCC19367 凍干粉                                         

QT-00139 絲狀鏈霉菌ATCC19753 凍干粉                                         

QT-00140 裂殖壺菌ATCC20888      凍干粉                                         

QT-00141 產(chǎn)朊假絲酵母ATCC22023      凍干粉                                         

QT-00142 乙酸鈣不動(dòng)桿菌ATCC23055 凍干粉                                         

QT-00143 珊瑚鏈霉菌ATCC23901 凍干粉                                         

QT-00144 菲律賓鏈霉菌ATCC23905      凍干粉                                         

QT-00146 里氏木霉ATCC24449      凍干粉                                         

QT-00147 變異鏈球菌ATCC25175 凍干粉                                         

QT-00148 沙鏈霉菌ATCC25488      凍干粉                                         

QT-00149 酪丁酸梭菌ATCC25755 凍干粉                                          【人細(xì)胞毒素(CTX)ELISA試劑盒現(xiàn)貨】

QT-00150 蠣灰鏈霉菌ATCC27455 凍干粉                                         

QT-00151 黃曲霉ATCC28539 凍干粉                                         

QT-00152 噬糖鹽紅菌ATCC29252 凍干粉                                         

133269     NeXtalStock Phosphate-citr. pH 4.2 (200)                 CNY                             

990522     Filter-Tips, 200 µl (960)            CNY                             

990396     QIAcube Modified Protocol               CNY                             

4501263  artus EBV RG PCR Kit (24) CE            CNY                             

4501205  artus EBV RG PCR Kit (96) RUO                 CNY                             

4500265  artus HSV-1/2 RG PCR Kit (96) CE               CNY                             

949008     Autopure Precipitation Soln (3800 ml)              CNY                             

335001     EpiTect Methyl qPCR Assay (200)              CNY                             

990332     Filter-Tips, 200 µl (1024)          CNY                             

939011     Buffer ATL ( 4 x 50 ml)               CNY                             

9010929  Motor, Y, with gearbox encoder                CNY                             

9010855  ZLV BGR DT-Abstreifer 4-fach Plato 7...D                  CNY                             

133246     NeXtalStock Sodium phosphate pH9.2 (200)            CNY                              【人細(xì)胞毒素(CTX)ELISA試劑盒現(xiàn)貨】

330601     RT² SYBR Green FAST Mastermix (8)                 CNY                             

929604     QX Wash Buffer (40 ml)             CNY                             

9010810  Source rack (96 pos) for tubes 16mm x 1                   CNY                             

9010636  Microplate station, tall    Station for accommodating a 96-well plate (or other labware with the same base dimensions) on the worktable  CNY                             

69581       DNeasy 96 Blood & Tissue Kit (4)    For 4 x 96 DNA minipreps: 4 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers       CNY                             

63206       DyeEx 2.0 Spin Kit (250)  250 DyeEx Spin Columns, Collection Tubes (2 ml)    CNY                             

207043     QuantiTect Whole Transcriptome Kit (25)                 CNY                             

9010253  Gripper, right, landoplasted, MTP 120             CNY                             

979101     PyroMark Q96 HS Plate (100)  For sample analysis of DNA template on MD. CNY                             

979009     PyroMark Annealing Buffer (250 ml)        For annealing of sequencing primer to DNA template.   CNY                             

62124       EZ1 DSP DNA Blood Kit (48)     CE-IVD Certified Kit for Extraction of DNA from human whole Blood. To be used in combination with EZ1 DSP Instrument (SAP 9001360) ａnd EZ1 DSP Blood Card (SAP 9017713). Available Input Volumes 350 µl, selectable elution volumes 200 µl. Processing time for 6 samples 25 Minutes.       CNY                             

301709     HiPerFect Transfection Reagent (100 ml)                  CNY                             

9002199  RGQ 6plex, PC, PrioPLUS, no PM     "RGQ 6plex, PC Instrument

Installation of RGQ 2plex & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

Loaner available in 2 days*

No preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"          CNY                             

9002196  RGQ 2plex HRM, PC, PrioPLUS, basic,no PM   "RGQ 2plex HRM, PC Instrument

Installation of RGQ 2plex HRM & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10

Loaner available in 2 days*

Preventive Maintenance (RRC,  1 PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"          CNY                             

206546     Type-it HRM PCR Kit (2000)              CNY                             

206541     Rotor-Gene Type-it HRM Discovery Kit            CNY                              【人細(xì)胞毒素(CTX)ELISA試劑盒現(xiàn)貨】

9012731  Diluent Trough + lid PK 80 white               CNY                             

9012721  syringe pump inlet tubing Plato 3100                CNY                             

978803     PyroMark OneStep RT-PCR Kit (200)                 CNY                             

133231     NeXtalStock Sodium cacodyl. pH 6.8 (100)                CNY