【友情提示】：本產品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準備試劑，樣品和標準品

2.加入準備好的樣品和標準品，37℃反應30分鐘

3.洗板5次，加入酶標試劑，37℃反應30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內讀OD值

7.計算
齊一生物供應ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據。為了保證實驗數據的可靠性，在實驗過程中必須堅持全面質量控制和全過程質量控制。在收集標本前都必須有一個完整的計劃。

【人αN已酰氨基葡糖苷酶(αNAG)ELISA試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【人αN已酰氨基葡糖苷酶(αNAG)ELISA試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人αN已酰氨基葡糖苷酶(αNAG)ELISA試劑盒現(xiàn)貨】樣本離心轉速及時間

1.試管采集的樣本：3000轉，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應將其分成小部分-70 ℃保存，避免反復冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應在室溫下解凍并確保樣品均勻地充分解凍。

【人αN已酰氨基葡糖苷酶(αNAG)ELISA試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

       大鼠，小鼠，兔組織源性原代細胞總DNA      1μg/1μl（10μg/20ug/50ug）                              

       └ 肺血管內皮細胞總DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 肺血管平滑肌細胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ Ⅱ型肺泡上皮細胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 氣管上皮細胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 氣道平滑肌細胞總DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 肺成纖維細胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 支氣管上皮細胞總DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 支氣管成纖維細胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 心肌細胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 心肌成纖維細胞總DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 主動脈內皮細胞總DNA 1μg/1μl（10μg/20ug/50ug）                               【人αN已酰氨基葡糖苷酶(αNAG)ELISA試劑盒現(xiàn)貨】

       └ 主動脈平滑肌細胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 大隱靜脈平滑肌細胞總DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 冠狀動脈平滑肌細胞總DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 食管上皮細胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 食管平滑肌細胞總DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 腸平滑肌細胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 腸粘膜上皮細胞總DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 腸動脈內皮細胞總DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 腸靜脈內皮細胞總DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 肝實質細胞總DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 肝動脈內皮細胞總DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 肝動脈平滑肌細胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 小腸血管內皮細胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 小腸隱窩上皮細胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 肝內膽管上皮細胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 胃粘膜上皮細胞總DNA 1μg/1μl（10μg/20ug/50ug）                               【人αN已酰氨基葡糖苷酶(αNAG)ELISA試劑盒現(xiàn)貨】

       └ 肝竇內皮細胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

       └ 腦動脈血管內皮細胞總DNA 1μg/1μl（10μg/20ug/50ug）                              

       └ 腦動脈血管平滑肌細胞總DNA    1μg/1μl（10μg/20ug/50ug）                              

9238657  Main cable, centrifuge           CNY                           

9238630  Jumper, 2-channel         CNY                           

9238620  Computer board Zent3, no Eprom        CNY                           

9242063  Evaporation trap, IQ/OQ, QS        CNY                           

9241890  QIArent&go - Rent out fee EZ1 XL             CNY                           

9241825  Pyro Q96 MDa, Full Agreement    The Full Agreement, PyM provides full service coverage for the PyroMark instrument. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one on-site inspection service, with costs for labor ａnd parts covered. With a Full Agreement, there is a response time of 2 working days.   CNY                           

9241167  Board, LED scan, QX           CNY                           

9241148  Transport lock, Y axis, M48         CNY                           

9241143  Application AUTOPURE (customized)         CNY                           

9241005  QIArent&go - Rent out fee BR Universal             CNY                           

9240987  Kit, electronic, FFC-plugs, Qsym         CNY                           

9240980  Sensor, tray, sample tracking, Qsym            CNY                           

9240945  Sensor, xy, portal, Qsym              CNY                            【人αN已酰氨基葡糖苷酶(αNAG)ELISA試劑盒現(xiàn)貨】

9234856  Tubing coupling piece for washer liquid              CNY                           

9234852  Card, ISDN, Fritz PCI          CNY                           

9240783  Update, Weigh Cell, AP96             CNY                           

9240774  Assy, Transfer Rack, AP96          CNY                           

9241632  LED Light System, AP          CNY                           

9241369  Temperature Controller, EZ1a              CNY                           

9243645  Guided Valid Support, QIA Extraction Sys    The QIAGEN Other Extraction System Guided Validation Support service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to QIAGEN extraction chemistry utilized in the process, review the appropriate Handbook ａnd User Manual, as well as hands-on chemistry ａnd instrument operation as required.  The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to validate (Biosprint 96, DNA mericon)    CNY                           

9243542  BR MDx, PM Service    "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                           

9243305  Power Cord, India         CNY                           

9243110  CD-RW, Black, AP196/98             CNY                           

9242712  Validation Support, 2d QIAcube    The QIAGEN QIAcube Guided Validation includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist. A QIAGEN specialist will provide an introduction to the QIAcube system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on QIAcube operation.  The Guided Validation service includes experimental design consulation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.  Please indicate which protocols to include in the validation (QIAamp, QIAamp DNA Investigator, MinElute PCR Cleanup, etc.)    CNY                           

9019226  Fan - rear 92x92, PyroQ24           CNY                           

9019220  PCB - Distribution robot, PyroQ96MD        CNY                           

1066843  PG Influenza A RT-PCR Assay (48) LE Z            CNY                           

1066848  NDV Univ. RT-PCR REAGENT LE             CNY                           

1066854  AIV-H9 RT-PCR REAGENT LE           CNY                            【人αN已酰氨基葡糖苷酶(αNAG)ELISA試劑盒現(xiàn)貨】

1060992  Buffer MW1 (77ml)              CNY                           

1061059  Sample Preparation Reagents, 8ml        CNY                           

1061080  NG Quantification Standards        CNY                           

1061113  PG AIV-H7 RT-PCR Assay (48) Z        CNY