1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人β-促脂素(β-LPH)ELISA試劑盒現(xiàn)貨】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說(shuō)明！

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見(jiàn)的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問(wèn)世以來(lái),得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【人β-促脂素(β-LPH)ELISA試劑盒現(xiàn)貨】齊一生物科技?xì)g迎您訂購(gòu)咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人β-促脂素(β-LPH)ELISA試劑盒現(xiàn)貨】齊一生物供應(yīng)的ELISA試劑盒ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過(guò)的HRP.再經(jīng)過(guò)溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開(kāi)優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過(guò)各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說(shuō)（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開(kāi)放閱讀框2和開(kāi)放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過(guò)氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性. 【人β-促脂素(β-LPH)ELISA試劑盒現(xiàn)貨】

以上資料僅供參考了解，不供于論文寫(xiě)作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

DMEM     DMEM     DMEM with High-Glucose - L-Glutamine ａnd Sodium Pyruvate      500 ml                                 

DMEM  DMEM  DMEM with High-Glucose - L-Glutamine ａnd Sodium Pyruvate     1L                                

DMEM    DMEM    DMEM with L-Glutamine - Sodium Pyruvate ａnd 25 mM HEPES     500 ml                                 

DMEM  DMEM  DMEM with High-Glucose - L-Glutamine - Sodium Pyruvate ａnd 25 mM HEPES     500 ml                                 

DMEM-prf        DMEM-prf        DMEM with High Glucose - L-Glutamine ａnd Sodium Pyruvate - phenol red-free  1L                                

Ham＇s F-10       Ham＇s F-10       F-10 with L-Glutamine ａnd 25 mM HEPES      500 ml                                 

Ham＇s F-12       Ham＇s F-12       F-12 with L-Glutamine ａnd 25 mM HEPES      500 ml                                 

DMEM/F-12     DMEM/F-12     DMEM/F-12 with L-Glutamine        500 ml                                  【人β-促脂素(β-LPH)ELISA試劑盒現(xiàn)貨】

DMEM/F-12    DMEM/F-12    DMEM/F-12 with L-Glutamine ａnd 15 mM HEPES          500 ml                                 

RPMI 1640       RPMI 1640       RPMI  1640 without L-Glutamine - with 25 mM HEPES        500 ml                                 

RPMI 1640       RPMI 1640       RPMI  1640 with L-Glutamine ａnd 25 mM HEPES          500 ml                                 

IMDM    IMDM    MDM with L-Glutamine ａnd 25 mM HEPES; without alpha-Thioglycerol - 2-mercaptoethanol  500 ml                                 

rhSCF        重組人干細(xì)胞因子         Recombinant Human Stem Cell Factor    2µg                             

rhG-CSF    重組人粒細(xì)胞集落刺激因子         Recombinant Human Granulocyte Colony Stimulating Factor         2µg                             

rhGM-CSF         重組人粒細(xì)胞巨噬細(xì)胞集落刺激因子         Recombinant Human Granulocyte Macrophage Colony Stimulating Factor         5µg                                     

rhM-CSF   重組人巨噬細(xì)胞集落刺激因子     Recombinant Human Macrophage Colony Stimulating Factor        2µg                             

rhTNF-α 重組人腫瘤壞死因子-α        Recombinant Human Tumor Necrosis Factor-alpha         10µg                                    

rhTNF-α-His    重組人腫瘤壞死因子-α,His Recombinant Human Tumor Necrosis Factor-alpha,His  10µg                                    

rhTNF-α Vari 重組人腫瘤壞死因子-αVariant Recombinant Human Tumor Necrosis Factor- alpha Variant          10µg                                    

rhBAF       重組人B細(xì)胞活化因子         Recombinant Human B Cell Activating Factor         5µg                                     

rhOSM      重組人抑瘤素-M    Recombinant Human Oncostatin-M        2µg                             

rhOSM 209a.a.         重組人抑瘤素-M209a.a.        Recombinant Human Oncostatin-M 209a.a.  2µg                             

rhAK1-3    重組人血管抑素K1-3     Recombinant Human Angiostatin K1-3   10µg                                    

rhEndostatin    重組人血管內(nèi)皮抑素     Recombinant Human Endostatin     2µg                              【人β-促脂素(β-LPH)ELISA試劑盒現(xiàn)貨】

rhaFGF     重組人成纖維細(xì)胞生長(zhǎng)因子－－酸性       Recombinant Human Fibroblast Growth Factor- acidic  10µg                                    

rhbFGF     重組人成纖維細(xì)胞生長(zhǎng)因子－－堿性       Recombinant Human Fibroblast Growth Factor-basic    10µg                                    

rhKGF-1    重組人角質(zhì)細(xì)胞生長(zhǎng)因子-1 Recombinant Human Keratinocye Growth Factor-1        2µg                             

rhFGF-9    重組人成纖維細(xì)胞生長(zhǎng)因子-9      Recombinant Human Fibrobalst Growth Factor-9  5µg                                     

rhKGF-2    重組人角質(zhì)細(xì)胞生長(zhǎng)因子-2 "Recombinant Human Keratinocye Growth Factor-2

"       5µg                             

rhFGF-19  重組人成纖維細(xì)胞生長(zhǎng)因子-19   "Recombinant Human Fibrobalst Growth Factor-19

"       5µg                                     

rhFGF-21  重組人成纖維細(xì)胞生長(zhǎng)因子-21   "Recombinant Human Fibrobalst Growth Factor-21

"       5µg                                     

9236166  Safety shield, RapidPlate          CNY                              【人β-促脂素(β-LPH)ELISA試劑盒現(xiàn)貨】

9230863  Anpressplatte Rose-80              CNY                             

9235538  Teaching Tool            CNY                             

9230282  Cable, RS323, robot to syringe pump, 225                CNY                             

5050-2002        hc2 Software V2 Package                  CNY                             

BS96RNA BioSprint 96 to RNA (384) - UDP               CNY                             

9240730  Tubing, 1/4" OD, Red, AP          CNY                             

9240713  Poly Muffler Kit, AP96/196                 CNY                             

9240690  Bearings, Lead Screws, AP                CNY                             

9243654  Comprehen Valid., Other Quant. Sys.      """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   For planning purposes ａnd to ensure a smooth process, the Customer is expected to have all materials on-hand ａnd inventoried one (1) week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """     CNY                             

6000-5013        LumiCheck Plate User Package                  CNY                             

288103     bactotype Mycoplasma Mg/Ms PCR Kit (24)            CNY                             

1048147  QIAamp Viral RNA Mini Accessory Set              CNY                             

270043     cattletype BHV1 gB Ab (5)                CNY                             

1019992  Buffer Set (RNeasy 96 BioRobot 9604 Kit                  CNY                             

273401     pigtype Toxoplasma Ab (1)                CNY                             

273505     pigtype Trichinella Ab (20)                 CNY                              【人β-促脂素(β-LPH)ELISA試劑盒現(xiàn)貨】

9019538  ZLVBelting, flat cogged, 6T, 2.5mm, CASx                CNY                             

9020591  Tray Assembly, DML 3000                  CNY                             

9021007  Motor, Pipettor X, DCU             CNY                             

9013373  Fix kit, liquid handling, BR8000                 CNY                             

9013315  Motor, handler T/L, BR3000             CNY                             

9013262  Cable, 24 V, power to power, 1200 mm           CNY                             

9013249  BGR Wash System             CNY                             

9013207  Service kit, accessories, BR8000               CNY                             

9013194  Tubing, L0 outlet, auto vac,BR8000 v1.00                CNY                             

9013187  Holder, retractable tubing, dispenser, B          CNY                             

800328     Miniprep, PCR purification (Easy-Read)            CNY                             

132109     EasyXtal 15-Well DG-Tool X-Seal (100)     "100 empty 15-well crystallization plates with dropguard, X-Seal crystallization supports

For manual setup of hanging ｄrop crystallization experiments."    CNY                             

132101     NeXtal DWBlock Adhesive Foils                 CNY                             

132045     NeXtal Evolution µplate (10)             CNY                             

338172     RT2 Calibration Plate A             CNY                             

338125     384EZload Covers (4)                 CNY                              【人β-促脂素(β-LPH)ELISA試劑盒現(xiàn)貨】

FM   成纖維細(xì)胞培養(yǎng)基         Fibroblast Medium  500 ml                                 

FM-2         成纖維細(xì)胞培養(yǎng)基-2    Fibroblast Medium-2        500 ml                                 

AEpiCM    肺泡上皮細(xì)胞培養(yǎng)基     Alveolar Epithelial Cell Medium       500 ml                                  【人基質(zhì)裂解素(ST3)ELISA試劑盒現(xiàn)貨】

SkMCM    骨骼肌細(xì)胞培養(yǎng)基       Skeletal Muscle Cell Medium   500 ml                                 

EpiCM       上皮細(xì)胞培養(yǎng)基  Epithelial Cell Medium     500 ml                                 

EpiCM-a   動(dòng)物上皮細(xì)胞培養(yǎng)基  Epithelial Cell Medium-animal          500 ml                                 

MCM        腎系膜細(xì)胞培養(yǎng)基       Mesangial Cell Medium   500 ml                                 

ObM         成骨細(xì)胞培養(yǎng)基  Osteoblast Medium 500 ml                                 

CM   軟骨細(xì)胞培養(yǎng)基     Chondrocyte Medium      500 ml                                 

SM   滑膜細(xì)胞培養(yǎng)基  Synoviocyte Medium        500 ml                                 

NPCM       髓核細(xì)胞培養(yǎng)基  Nucleus Pulposus Cell Medium         500 ml                                 

HM  肝細(xì)胞培養(yǎng)基       Hepatocyte Medium        500 ml                                 

SteCM      星形細(xì)胞培養(yǎng)基  Stellate Cell Medium        500 ml                                 

CMM        心肌細(xì)胞培養(yǎng)基  Cardiac Myocyte Medium        500 ml                                 

TMCM      小梁網(wǎng)細(xì)胞培養(yǎng)基         "Trabecular Meshwork Cell Medium

"       500 ml                                 

TM   滋養(yǎng)層細(xì)胞培養(yǎng)基       Trophoblast Medium        500 ml                                 

AdM          脂肪細(xì)胞培養(yǎng)基     Adipocyte Medium  500ml                                  

PAM 前脂肪細(xì)胞培養(yǎng)基       Preadipocyte Medium     500 ml                                 

PADM       脂肪細(xì)胞誘導(dǎo)分化培養(yǎng)基     Preadipocyte Differentiation Medium     500 ml                                  【人基質(zhì)裂解素(ST3)ELISA試劑盒現(xiàn)貨】

MSCM      間充質(zhì)干細(xì)胞培養(yǎng)基  Mesenchymal Stem Cell Medium    500 ml                                 

MODM-500      間充質(zhì)干細(xì)胞-成骨細(xì)胞分化培養(yǎng)基 Mesenchymal Stem Cell Oseogenic Differentiation Medium  500 ml                                 

MODM-250               Mesenchymal Stem Cell Oseogenic Differentiation Medium  250 ml                                 

MADM-500      間充質(zhì)干細(xì)胞-脂肪細(xì)胞分化培養(yǎng)基   Mesenchymal Stem Cell Adipogenic Differentiation Medium 500 ml                                 

MADM-250               Mesenchymal Stem Cell Adipogenic Differentiation Medium 250 ml                                 

MCDM     間充質(zhì)干細(xì)胞-軟骨分化培養(yǎng)基   Mesenchymal Stem Cell Chondrogenic Differentiation Medium     500ml                                  

SMCM-sf  平滑肌細(xì)胞培養(yǎng)基         Smooth Muscle Cell Medium- serum free        500 ml                                 

NPrCM     神經(jīng)前體細(xì)胞培養(yǎng)基     Neural Precursor Cell Medium         500 ml                                 

NM  神經(jīng)細(xì)胞培養(yǎng)基  Neuronal Medium   500 ml                                 

1061071  PG AIV-H5 RT-PCR Assay (48)  Z              CNY                             

1061075  PG NDV Univ. RT-PCR Assay (48) Z            CNY                             

1061085  TB PCR REAGENT II          CNY                              【人基質(zhì)裂解素(ST3)ELISA試劑盒現(xiàn)貨】

1061098  HIV-1 RT-PCR REAGENT II                  CNY                             

9016574  QIAamp Media MDx, PRO                 CNY                             

9016538  Transportation box , BioRobot EZ1           CNY                             

9016528  Transportation box , Access. Cabinet                CNY                             

9016471  BR MDx Service, PRO                CNY                             

9016150  #App. Package, M48, Forensics                 CNY                             

9016146  #App. Package, M48, Genotyping            CNY                             

9019182  Pump - Pressure, PyroQ96                 CNY                             

9019172  Contact pins - Heating block, PyroQ96              CNY                             

9011910  Tip-disposal unit, v2.03             CNY                             

9011893  Tubing, teflon, M6, 2.5 x 0.5 mm, 0.25 m                 CNY                             

9237081  Installation, BR plus 1 integration            CNY                             

9236921  Liquid Sensor, BR8 RD v1.0                CNY                             

9019148  IPC chassis, PyroQ96MD           CNY                             

9019076  PyroMark CDT Holder, PyroQ96MD                  CNY                             

9019006  Power supply, EZ1xl          CNY                              【人基質(zhì)裂解素(ST3)ELISA試劑盒現(xiàn)貨】

9018997  Cover, front, left, EZ1xl             CNY                             

9018957  Reagent Block, 17x5ml Flat -Base Tubes          CNY                             

9018936  Adapter, 16 tube-rack cepheid Smartcycle               CNY                             

9022748  Actuator, Tip Eject, DCU          CNY                             

9022733  RQ document, RGQ          CNY                             

9022708  Baffle, PMT, DML3000              CNY                             

9016065  Maintenance Kit, Basic, BR8000 I            CNY                             

9016063  Tubing Set, Waste DISU, BR8000              CNY                             

9015724  Probe, 0.9 mm, BR8000 v1.00                   CNY                             

9015534  Cover, front, type B          CNY                             

9015529  Filter, inlet, with switch & fuse                  CNY                             

9014202  Tubing Sys., Bottle, BR8 RD              CNY                             

9021194  Grid, fan, SP, Qsym            CNY                             

9021128  Cooling adapter, PCR 384, Qsym               CNY