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齊一生物現(xiàn)貨供應(yīng)人ELISA試劑盒，大鼠ELISA試劑盒，小鼠ELISA試劑盒等現(xiàn)貨充足，購(gòu)買任意ELISA試劑盒都享受免費(fèi)代測(cè)服務(wù)，更多進(jìn)口原裝品牌試劑盒供應(yīng)齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人蛋白酶3特異性抗中性粒細(xì)胞胞質(zhì)抗體(PR3-ANCA)ELISA試劑盒廠家】酶法檢測(cè)試驗(yàn)所需自備物品:

1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

【人蛋白酶3特異性抗中性粒細(xì)胞胞質(zhì)抗體(PR3-ANCA)ELISA試劑盒廠家】操作注意事項(xiàng)：

1.試劑盒應(yīng)按標(biāo)簽說明書儲(chǔ)存，使用前恢復(fù)到室溫30分鐘后方可使用。稀稀過后的標(biāo)準(zhǔn)品應(yīng)丟棄，不可保存。

2.實(shí)驗(yàn)中不用的板條應(yīng)立即放回包裝袋中，密封保存，以免變質(zhì)。

3.不用的其它試劑應(yīng)包裝好或蓋好。不同批號(hào)的試劑不要混用.請(qǐng)?jiān)谠噭┖斜Ｙ|(zhì)前使用。

4.使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時(shí)，避免使用帶金屬部分的加樣器。

5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。

6.洗滌酶標(biāo)板時(shí)應(yīng)充分拍干，不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。

7.底物A應(yīng)揮發(fā)，避免長(zhǎng)時(shí)間打開蓋子。底物B對(duì)光敏感，避免長(zhǎng)時(shí)間暴露于光下。避免用手接觸，有毒。實(shí)驗(yàn)完成后應(yīng)立即讀取OD值。

8.加入試劑的順序應(yīng)一致，以保證所有反應(yīng)板孔溫育的時(shí)間一樣。

9.按照說明書中標(biāo)明的時(shí)間、加液的量及順序進(jìn)行溫育操作。

【人蛋白酶3特異性抗中性粒細(xì)胞胞質(zhì)抗體(PR3-ANCA)ELISA試劑盒廠家】齊一生物客戶寄標(biāo)本時(shí)需注明以下情況：

1、標(biāo)本編號(hào)；2、所測(cè)項(xiàng)目；3、是否做復(fù)孔；3、聯(lián)系方式；4、實(shí)驗(yàn)后標(biāo)本是否寄回。

客戶必知：

客戶應(yīng)對(duì)所提供的材料及信息負(fù)責(zé)，如因客戶提供的材料及信息不準(zhǔn)確而引起的實(shí)驗(yàn)延誤或經(jīng)濟(jì)損失由客戶承擔(dān)。

齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人蛋白酶3特異性抗中性粒細(xì)胞胞質(zhì)抗體(PR3-ANCA)ELISA試劑盒廠家】齊一生物供應(yīng)，以上資料供參考如有疑問需要詳細(xì)資料請(qǐng)聯(lián)系我們客服人員，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

OPCM       少突膠質(zhì)前體細(xì)胞培養(yǎng)基  Oligodendrocyte Precursor Cell Medium          500 ml                                 

OsM 球狀少突細(xì)胞培養(yǎng)基  Oligosphere Medium        500 ml                                 

KM   角質(zhì)細(xì)胞培養(yǎng)基  Keratinocyte Medium      500 ml                                 

KM-d         角質(zhì)細(xì)胞培養(yǎng)基（確定）          Keratinocyte Medium-defined         500 ml                                 

FM-sf        成纖維細(xì)胞培養(yǎng)基         Fibroblast Medium-serum free        500 ml                                 

TEpiCM    扁桃體上皮細(xì)胞培養(yǎng)基         Tonsil Epithelial Cell Medium   500 ml                                 

OKM         口腔角質(zhì)細(xì)胞培養(yǎng)基  Oral Keratinocyte Medium      500 ml                                  【人蛋白酶3特異性抗中性粒細(xì)胞胞質(zhì)抗體(PR3-ANCA)ELISA試劑盒廠家】

CoEpiCM  結(jié)腸上皮細(xì)胞培養(yǎng)基     Colonic Epithelial Cell Medium         500 ml                                 

BEpiCM    支氣管上皮細(xì)胞培養(yǎng)基       Bronchial Epithelial Cell Medium     500 ml                                 

SAEpiCM  氣管上皮細(xì)胞培養(yǎng)基  Small Airway Epithelial Cell Medium        500 ml                                 

EpiCM-2   上皮細(xì)胞培養(yǎng)基-2        Epithelial Cell Medium-2 500 ml                                 

UCM         尿道上皮細(xì)胞培養(yǎng)基  Urothelial Cell Medium    500 ml                                 

PEpiCM    前列腺上皮細(xì)胞培養(yǎng)基       Prostate Epithelial Cell Medium       500 ml                                 

STEMium 人胚胎干細(xì)胞無(wú)血清培養(yǎng)基         Human Pluripotent Stem Cell Growth Medium       500 ml                                 

STEMium-XF     無(wú)異種人胚胎干細(xì)胞生長(zhǎng)培養(yǎng)基         Xeno-free Human Pluripotent Stem Cell Growth Medium       500 ml                                 

MEF-cm   小鼠胚胎成纖維細(xì)胞培養(yǎng)基         Mouse Embryonic Fibroblasts-Conditioned Medium        100 ml                                

bFGF-std MEF-cm     堿性成纖維細(xì)胞生長(zhǎng)因子刺激的小鼠胚胎成纖維細(xì)胞條件培養(yǎng)基         bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium     100ml                                  

CEpiCM    角膜上皮細(xì)胞培養(yǎng)基  Corneal Epithelial Cell Medium        500 ml                                 

OEpiCM    卵巢上皮細(xì)胞培養(yǎng)基  Ovarian Epithelial Cell Medium        500 ml                                 

MSCM-sf  間充質(zhì)干細(xì)胞培養(yǎng)基  Mesenchymal Stem Cell Medium-serum free 500 ml                                 

MEpiCM   乳腺上皮細(xì)胞培養(yǎng)基  Mammary Epithelial Cell Medium   500 ml                                 

KM-acf     角質(zhì)細(xì)胞培養(yǎng)基  Keratinocyte Medium-animal component free        500 ml                                 

FM-acf      成纖維細(xì)胞培養(yǎng)基       Fibroblast Medium-animal component free    500 ml                                  【人蛋白酶3特異性抗中性粒細(xì)胞胞質(zhì)抗體(PR3-ANCA)ELISA試劑盒廠家】

STEMium-ACF  人類多能干細(xì)胞培養(yǎng)基-無(wú)動(dòng)物組分   STEMium® Human Pluripotent Stem Cell Growth Medium-animal component free          500ml                                  

MSCM-acf         間充質(zhì)干細(xì)胞培養(yǎng)基     Mesenchymal Stem Cell Medium-animal component free        500 ml                            

MEM        MEM        MEM with Earle＇s Salts ａnd L-Glutamine        500 ml                                 

MEM       MEM       MEM with Hank＇s Salts ａnd L-Glutamine         500 ml                                 

MEM       MEM       MEM with Eagles＇s Salts ａnd Glutamine         500 ml                                 

M199E  M199E  Medium 199 with Earle＇s Salts ａnd L-Glutamine    500 ml                                 

M199H           M199H           Medium 199 with Hanks Salts ａnd L-Glutamine     500 ml                                 

DMEM    DMEM    DMEM with L-Glutamine ａnd Sodium Pyruvate     500 ml                                 

9021101  Skin Panel, Lower Left Rear, DCU             CNY                             

9238279  PAXgene 96 Incubator Block (farbl.elox)          CNY                              【人蛋白酶3特異性抗中性粒細(xì)胞胞質(zhì)抗體(PR3-ANCA)ELISA試劑盒廠家】

9240677  Bumper, Round, Latch Lock, AP98            CNY                             

9240635  Filter, EMI,Modular, RJ45, AP196/98                 CNY                             

9240615  Fuse, 1.0A, FNM, AP96             CNY                             

9240612  Turk Sensor, Waste Bottle Level, AP                  CNY                             

9240603  Deck/Waste Overflow Sensor, AP196/98           CNY                             

9240583  Regulator, Pressure, Main, AP                   CNY                             

9240545  Hatch pad, Eppendorf, AP196/98               CNY                             

9240542  Lower Header, Capper Head, AP196                 CNY                             

9240521  Motor Assy, Shaker, AP98                  CNY                             

9240520  Motor Assy, Sz. 17, Common, AP98                   CNY                             

9240102  Re-Installation, LiquiChip          The Re-Installation, LiquiChip facilitates quick implementation of system back into lab routine by minimizing instrument set-up phase. It covers hardware ａnd software check of instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.          CNY                             

9019940  Power supply, vac pump, CX1           CNY                             

9019477  LASER MODUAL GS          CNY                             

9019476  Spacer, 0.2 mm short set, GSC                   CNY                             

9238888  PCB, Main-01, BS96                   CNY                             

9238886  Cable, HM, BS96               CNY                              【人蛋白酶3特異性抗中性粒細(xì)胞胞質(zhì)抗體(PR3-ANCA)ELISA試劑盒廠家】

9238854  #BR M96, Basic Agreement    The Basic Cover Agreement M96 provides full service coverage for the BioRobot M96. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238842  RS232 box, non-online fill, RapidPlate               CNY                             

9241215  Full Agreement Depot Repair, Shaker, 1yr       "The Full Agreement Depot Repair, Shaker provides full service coverage for the plate shaker used in the digene HPV application. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."          CNY                             

9238833  #BR 9604, Basic Agreement    The Basic Cover Agreement 9604, 1 PM provides full service coverage for the BioRobot 9604. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9238832  BR 9600, Basic Agreement      The Basic Cover Agreement 9600 provides full service coverage for the BioRobot 9600. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238819  Rect. carrier for 5 glass tubes 25ml                   CNY                             

9237527  Software LiquiChip Workstation IS V2.2.          CNY                             

9237380  Installation, BR MDx & 2 react. setup     Installation ａnd training, including two customized reaction setup protocols         CNY                             

9237379  Installation, BR MDx & 1 react. setup     Installation ａnd training, including one customized reaction setup protocol CNY                             

9237378  BR MDx, Installation        The Installation, BR MDx facilitates the quick implementation of the BioRobot MDx into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the BioRobot MDx instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9237175  Guard Fan, Platic XYP                CNY                             

9233209  Tubing, 3/2,                CNY                             

9233155  Power Cord, European              CNY                             

9236418  Board, CPU, TWI               CNY                             

9236345  Red LSR             CNY                             

　　測(cè)定小分子量物質(zhì)常用競(jìng)爭(zhēng)法，其標(biāo)準(zhǔn)曲線中吸光度與受檢物質(zhì)的濃度呈負(fù)相關(guān)。標(biāo)準(zhǔn)曲線的形狀因試劑盒所用模式的差別而略有不同。ELISA測(cè)定的標(biāo)準(zhǔn)曲線注意圖中橫坐標(biāo)為對(duì)數(shù)關(guān)系，這更有利于測(cè)定系統(tǒng)的表達(dá)。

        講述完了以上ELISA實(shí)驗(yàn)的操作要點(diǎn)，可以發(fā)現(xiàn)其實(shí)很多步驟都并不復(fù)雜，重要的是操作的時(shí)候一定要注重細(xì)節(jié)，熟能生巧，才可以掌握ELISA實(shí)驗(yàn)的精髓所在。

MaM        巨噬細(xì)胞的培養(yǎng)基         Macrophage Medium      500 ml                                 

MelM       黑色素細(xì)胞培養(yǎng)基       Melanocyte Medium        500 ml                                 

MelM-2    黑色素細(xì)胞培養(yǎng)基-2   Melanocyte Medium-2    500 ml                                 

FM   成纖維細(xì)胞培養(yǎng)基         Fibroblast Medium  500 ml                                 

FM-2         成纖維細(xì)胞培養(yǎng)基-2    Fibroblast Medium-2        500 ml                                 

AEpiCM    肺泡上皮細(xì)胞培養(yǎng)基     Alveolar Epithelial Cell Medium       500 ml                                  【人基質(zhì)裂解素(ST3)ELISA試劑盒現(xiàn)貨】

SkMCM    骨骼肌細(xì)胞培養(yǎng)基       Skeletal Muscle Cell Medium   500 ml                                 

EpiCM       上皮細(xì)胞培養(yǎng)基  Epithelial Cell Medium     500 ml                                 

EpiCM-a   動(dòng)物上皮細(xì)胞培養(yǎng)基  Epithelial Cell Medium-animal          500 ml                                 

MCM        腎系膜細(xì)胞培養(yǎng)基       Mesangial Cell Medium   500 ml                                 

ObM         成骨細(xì)胞培養(yǎng)基  Osteoblast Medium 500 ml                                 

CM   軟骨細(xì)胞培養(yǎng)基     Chondrocyte Medium      500 ml                                 

SM   滑膜細(xì)胞培養(yǎng)基  Synoviocyte Medium        500 ml                                 

NPCM       髓核細(xì)胞培養(yǎng)基  Nucleus Pulposus Cell Medium         500 ml                                 

HM  肝細(xì)胞培養(yǎng)基       Hepatocyte Medium        500 ml                                 

SteCM      星形細(xì)胞培養(yǎng)基  Stellate Cell Medium        500 ml                                 

CMM        心肌細(xì)胞培養(yǎng)基  Cardiac Myocyte Medium        500 ml                                 

TMCM      小梁網(wǎng)細(xì)胞培養(yǎng)基         "Trabecular Meshwork Cell Medium

"       500 ml                                 

TM   滋養(yǎng)層細(xì)胞培養(yǎng)基       Trophoblast Medium        500 ml                                 

AdM          脂肪細(xì)胞培養(yǎng)基     Adipocyte Medium  500ml                                  

PAM 前脂肪細(xì)胞培養(yǎng)基       Preadipocyte Medium     500 ml                                 

PADM       脂肪細(xì)胞誘導(dǎo)分化培養(yǎng)基     Preadipocyte Differentiation Medium     500 ml                                  【人基質(zhì)裂解素(ST3)ELISA試劑盒現(xiàn)貨】

MSCM      間充質(zhì)干細(xì)胞培養(yǎng)基  Mesenchymal Stem Cell Medium    500 ml                                 

MODM-500      間充質(zhì)干細(xì)胞-成骨細(xì)胞分化培養(yǎng)基 Mesenchymal Stem Cell Oseogenic Differentiation Medium  500 ml                                 

MODM-250               Mesenchymal Stem Cell Oseogenic Differentiation Medium  250 ml                                 

MADM-500      間充質(zhì)干細(xì)胞-脂肪細(xì)胞分化培養(yǎng)基   Mesenchymal Stem Cell Adipogenic Differentiation Medium 500 ml                                 

MADM-250               Mesenchymal Stem Cell Adipogenic Differentiation Medium 250 ml                                 

MCDM     間充質(zhì)干細(xì)胞-軟骨分化培養(yǎng)基   Mesenchymal Stem Cell Chondrogenic Differentiation Medium     500ml                                  

SMCM-sf  平滑肌細(xì)胞培養(yǎng)基         Smooth Muscle Cell Medium- serum free        500 ml                                 

NPrCM     神經(jīng)前體細(xì)胞培養(yǎng)基     Neural Precursor Cell Medium         500 ml                                 

NM  神經(jīng)細(xì)胞培養(yǎng)基  Neuronal Medium   500 ml                                 

1061071  PG AIV-H5 RT-PCR Assay (48)  Z              CNY                             

1061075  PG NDV Univ. RT-PCR Assay (48) Z            CNY                             

1061085  TB PCR REAGENT II          CNY                              【人基質(zhì)裂解素(ST3)ELISA試劑盒現(xiàn)貨】

1061098  HIV-1 RT-PCR REAGENT II                  CNY                             

9016574  QIAamp Media MDx, PRO                 CNY                             

9016538  Transportation box , BioRobot EZ1           CNY                             

9016528  Transportation box , Access. Cabinet                CNY                             

9016471  BR MDx Service, PRO                CNY                             

9016150  #App. Package, M48, Forensics                 CNY                             

9016146  #App. Package, M48, Genotyping            CNY                             

9019182  Pump - Pressure, PyroQ96                 CNY                             

9019172  Contact pins - Heating block, PyroQ96              CNY                             

9011910  Tip-disposal unit, v2.03             CNY                             

9011893  Tubing, teflon, M6, 2.5 x 0.5 mm, 0.25 m                 CNY                             

9237081  Installation, BR plus 1 integration            CNY                             

9236921  Liquid Sensor, BR8 RD v1.0                CNY                             

9019148  IPC chassis, PyroQ96MD           CNY                             

9019076  PyroMark CDT Holder, PyroQ96MD                  CNY                             

9019006  Power supply, EZ1xl          CNY                              【人基質(zhì)裂解素(ST3)ELISA試劑盒現(xiàn)貨】

9018997  Cover, front, left, EZ1xl             CNY                             

9018957  Reagent Block, 17x5ml Flat -Base Tubes          CNY                             

9018936  Adapter, 16 tube-rack cepheid Smartcycle               CNY                             

9022748  Actuator, Tip Eject, DCU          CNY                             

9022733  RQ document, RGQ          CNY                             

9022708  Baffle, PMT, DML3000              CNY                             

9016065  Maintenance Kit, Basic, BR8000 I            CNY                             

9016063  Tubing Set, Waste DISU, BR8000              CNY                             

9015724  Probe, 0.9 mm, BR8000 v1.00                   CNY                             

9015534  Cover, front, type B          CNY                             

9015529  Filter, inlet, with switch & fuse                  CNY                             

9014202  Tubing Sys., Bottle, BR8 RD              CNY                             

9021194  Grid, fan, SP, Qsym            CNY                             

9021128  Cooling adapter, PCR 384, Qsym               CNY