檢測(cè)標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測(cè)方法：ELISA

檢測(cè)類(lèi)型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠(chéng)為您服務(wù)！本試劑盒用于測(cè)定血清,血漿及相關(guān)液體樣本中含量.

【人抗甲狀腺微粒體抗體(ATMA/TMAB)ELISA試劑盒現(xiàn)貨】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值）,通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

【人抗甲狀腺微粒體抗體(ATMA/TMAB)ELISA試劑盒現(xiàn)貨】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過(guò)氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測(cè)樣品孔.在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測(cè)定：以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）. 測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

【人抗甲狀腺微粒體抗體(ATMA/TMAB)ELISA試劑盒現(xiàn)貨】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開(kāi)封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說(shuō)明書(shū)的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說(shuō)明書(shū)有異,以英文說(shuō)明書(shū)為準(zhǔn).

【人抗甲狀腺微粒體抗體(ATMA/TMAB)ELISA試劑盒現(xiàn)貨】計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見(jiàn)應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

QY-x216   NCI-H661大細(xì)胞肺癌細(xì)胞    HTB-183                                       

QY-x217   ZR-75-1乳腺癌細(xì)胞        CRL-1500                                               

QY-x218   NCI-H292肺癌細(xì)胞淋巴結(jié)轉(zhuǎn)移    CRL-1848                                               

QY-x219   Hs 578T乳腺癌細(xì)胞       HTB-126                                       

QY-x220   U-937組織細(xì)胞淋巴瘤細(xì)胞  CRL-1593.2                                           

QY-x221   CFPAC-1胰腺癌細(xì)胞       CRL-1918                                               

QY-x222   A3淋巴細(xì)胞白血病細(xì)胞        CRL-2570                                                【人抗甲狀腺微粒體抗體(ATMA/TMAB)ELISA試劑盒現(xiàn)貨】

QY-x223   PANC-1胰腺癌細(xì)胞         CRL-1469                                               

QY-x224   Jurkat,Clone E6-1T 淋巴細(xì)胞白血病細(xì)胞     TIB-152                                        

QY-x225   SK-HEP-1肝癌細(xì)胞          HTB-52                                         

QY-x227   HL-60急性粒細(xì)胞白血病細(xì)胞       CCL-240                                        

QY-x228   HCCLM3肝癌細(xì)胞                                               

QY-x229   TT人甲狀腺癌細(xì)胞        CRL-1803                                               

QY-x230   PLC/PRF/5肝癌亞力山大細(xì)胞        CRL-8024                                               

QY-x231   SW579人甲狀腺癌細(xì)胞         HTB-107                                       

QY-x232   RKO結(jié)腸腺癌細(xì)胞          CRL-2577                                               

QY-x233   FaDu人咽鱗癌細(xì)胞        HTB-43                                         

QY-x234   NCI-N87人胃癌細(xì)胞       CRL-5822                                               

QY-x235   DT40雞淋巴瘤細(xì)胞                                                     

QY-x236   人腦微血管內(nèi)皮細(xì)胞                                                  

QY-x237   人腦血管平滑肌細(xì)胞                                                  

QY-x238   人腦血管周細(xì)胞                                                  

QY-x239   人脈絡(luò)叢內(nèi)皮細(xì)胞                                                        【人抗甲狀腺微粒體抗體(ATMA/TMAB)ELISA試劑盒現(xiàn)貨】

QY-x240   人脈絡(luò)叢上皮細(xì)胞                                                       

QY-x241   人脈絡(luò)叢成纖維細(xì)胞                                                  

QY-x242   人腦膜細(xì)胞                                                  

QY-x243   人神經(jīng)細(xì)胞                                                  

QY-x244   人小腦顆粒細(xì)胞                                                  

QY-x245   人少突膠質(zhì)前體細(xì)胞                                                  

QY-x246   人少突膠質(zhì)前體細(xì)胞人肺微血管內(nèi)皮細(xì)胞                                                       

QY-x247   人肺動(dòng)脈內(nèi)皮細(xì)胞                                                       

9238716  Seal for lid, 1350 mm                 CNY                              【人抗甲狀腺微粒體抗體(ATMA/TMAB)ELISA試劑盒現(xiàn)貨】

9238898  Front panel, BS15              CNY                             

9238699  Frontpanel w. keyboard foil (Zent1)                   CNY                             

9231793  Motor, gripper, handler, BR8000              CNY                             

9238690  Computer board Zent2, no Eprom            CNY                             

9231434  Probe, 0,4mm, ceramic coated for PK80                   CNY                             

9020071  Keypad, TLyser                   CNY                             

9020070  Controller 100-120V/200-240V, TLyser              CNY                             

9014996  Shaker, Highspeed Unit, BR8 RD               CNY                             

9014870  X-Motor, BR8            CNY                             

9014790  Tip Disposal Bags MDx    Autoclavable bags for collection ａnd disposal of used disposable tips, pack of 15   CNY                            

9014642  System Reagent Troughs (Qty 10)             CNY                             

9019188  Mirror, PyroQ96                CNY                             

1064181  EGFR RGQ PCR Kit (24) IUO              CNY                             

1017647  Carrier RNA(poly A) 12x1350µg-15-25°C,KG             CNY                             

1059454  ResPlex II Plus Panel (25) PRE           CNY                             

1055628  TopElute Fluid (60/60),KG                   CNY                              【人抗甲狀腺微粒體抗體(ATMA/TMAB)ELISA試劑盒現(xiàn)貨】

1061068  Sample Preparation Reagents, 30ml                 CNY                             

1061104  UU PCR REAGENT I           CNY                             

9016583  ZLV Pipetting Posit., RCS1                  CNY                             

9016561  BR MDx DSP Serv.pro Plasticware Kit, V1                 CNY                             

9016505  Rect. Bucket Set (4)                   CNY                             

9016486  Cable Dilutor bus, RS485, Kloehn              CNY                             

9016288  Cable, RS232, EZ1a           CNY                             

9019181  PCB 7 - Connection, PyroQ96MD              CNY                             

9019115  Fan - IPC, PyroQ96ID                 CNY                             

9019101  Rotor-Gene Q Software            CNY                             

9019079  PyroMark Assay Design SW 2.0 (5)  "All-in-one software for design of both PCR ａnd sequencing primers

Five additional software licenses for PyroMark AssayDesign SW 1.0"     CNY                             

9019071  PyroMark Thermoplate, PyroQ96MD      Holder for placement of PyroMark Q96 Plate on heat block during annealing step prior to PyroMark Q96 MD analysis.        CNY                             

9019017  Tray, EZ1xl                 CNY                             

9019008  Reflector, UV lamp, EZ1xl                  CNY                             

9018934  Adapter, 24x5ml flat base Tubes               CNY                             

9016014  X-Motor DC, Gear, 20V             CNY                              【人抗甲狀腺微粒體抗體(ATMA/TMAB)ELISA試劑盒現(xiàn)貨】