檢測(cè)標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測(cè)方法：ELISA

檢測(cè)類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠(chéng)為您服務(wù)！本試劑盒用于測(cè)定血清,血漿及相關(guān)液體樣本中含量.

【人抗心肌抗體(AMA)ELISA試劑盒廠家】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值）,通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人抗心肌抗體(AMA)ELISA試劑盒廠家】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過(guò)氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人抗心肌抗體(AMA)ELISA試劑盒廠家】

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測(cè)樣品孔.在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測(cè)定：以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）. 測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果. 【人抗心肌抗體(AMA)ELISA試劑盒廠家】

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說(shuō)明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說(shuō)明書有異,以英文說(shuō)明書為準(zhǔn).

計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

QY-x185   WEHI 22.1B淋巴細(xì)胞   TIB-54                                           

QY-x186   NIH/3T3胚胎成纖維細(xì)胞       CRL-1658                                               

QY-x187   MC3T3-E1 Subclone 14小鼠原成骨細(xì)胞        CRL-2594                                               

QY-x188   L6大鼠成肌細(xì)胞   CRL-1458                                               

QY-x189   H9c22-1大鼠心肌細(xì)胞  CRL-1446                                               

QY-x190   RSC96大鼠雪旺細(xì)胞      CRL-2765                                                【人抗心肌抗體(AMA)ELISA試劑盒廠家】

QY-x191   CHO/dhFr-中國(guó)倉(cāng)鼠二氫葉酸還原酶缺陷細(xì)胞    CRL-9096                                               

QY-x192   Lec1卵巢細(xì)胞         CRL-1735                                               

QY-x193   293T/17 [HEK 293T/17] 人胚腎細(xì)胞       CRL-11268                                            

QY-x194   AAV-293 胚腎細(xì)胞                                                      

QY-x195   hFOB 1.19SV40 轉(zhuǎn)染成骨細(xì)胞      CRL-11372                                            

QY-x196   CCD-1095Sk皮膚細(xì)胞   CRL-2122                                               

QY-x197   Hs 578Bst乳腺細(xì)胞        HTB-125                                       

QY-x198   NK-92人惡性非霍奇金淋巴瘤患者的自然殺傷細(xì)胞  CRL-2407                                               

QY-x199   NK-92MI人惡性非霍奇金淋巴瘤患者的自然殺傷細(xì)胞      CRL-2408                                               

QY-x200   HMy2.CIR人B 淋巴母細(xì)胞  CRL-1993                                               

QY-x201   OK負(fù)鼠opossum腎細(xì)胞        CRL-1840                                               

QY-x202   FO骨髓瘤細(xì)胞        CRL-1646                                               

QY-x203   RAW 264.7單核巨噬細(xì)胞白血病  TIB-71                                           

QY-x204   P19畸胎癌細(xì)胞      CRL-1825                                               

QY-x205   C6膠質(zhì)瘤細(xì)胞        CCL-107                                        

QY-x206   RBL-2H3白血病細(xì)胞      CRL-2256                                                【人抗心肌抗體(AMA)ELISA試劑盒廠家】

QY-x207   SiHa子宮頸癌細(xì)胞         HTB-35                                         

QY-x208   MG-63骨肉瘤細(xì)胞         CRL-1427                                               

QY-x209   HEC-1-B子宮內(nèi)膜腺癌細(xì)胞  HTB-113                                       

QY-x210   SW1353骨肉瘤細(xì)胞       HTB-94                                         

QY-x211   ES-2卵巢透明細(xì)胞癌細(xì)胞     CRL-1978                                               

QY-x212   Saos-2成骨肉瘤細(xì)胞      HTB-85                                         

QY-x213   MDA-MB-468乳腺癌細(xì)胞      HTB-132                                       

QY-x214   SK-MES-1肺鱗癌細(xì)胞     HTB-58                                         

QY-x215   MDA-MB-435S乳腺癌細(xì)胞    HTB-129                                       

9020997  ZS Pipetting Module, DCU                 CNY                             

9020909  Cable, UV curly cord, SMC, QGX                CNY                              【人抗心肌抗體(AMA)ELISA試劑盒廠家】

9020908  Hinge, lid, SMC, QGX                 CNY                             

9020643  Waste chute, straight, AS, Cabinet Qsym                  CNY                             

9020632  Kit, hinges (4), Cabinet Qsym            CNY                             

9020629  Door, waste, SP, Cabinet Qsym                  CNY                             

9020625  Kit, repair, Cabinet Qsym                   CNY                             

9020332  EPROM - Motherboard, RGQ           CNY                             

9020233  Foot assembly, rear, CAS           CNY                             

9020158  Insulation disc - chamber bottom, RG3            CNY                             

9020096  Locking Pin, TLyser 2                  CNY                             

9020085  Thrust Washer, TLyser               CNY                             

9239498  V-Housing, LX100              CNY                             

9239490  EX Laser Driver, Uniphase                  CNY                             

9239390  PCR Plate Adapter, M96           CNY                             

9236510  BR 3000, PM Service        The Overhaul covers all travel ａnd labor costs plus all necessary parts to perform a Preventative Maintenance,. The instrument will also be thoroughly checked ａnd inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.     CNY                             

9232764  Disposable Troughs, 20 / 30 ml (10)          Troughs holding up to 20 ml of liquid; pack of 10     CNY                             

9236472  BR 9600, Full Agreement         The Full Agreement 9600 provides full service coverage for the BioRobot 9600. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).  CNY                              【人抗心肌抗體(AMA)ELISA試劑盒廠家】

9236443  BR, Installation         The Installation, BR Universal facilitates the quick implementation of the BioRobot into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the BioRobot Universal instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9239155  Assembly, vertical axis, TWI              CNY                             

9239108  Assy, Sample Needle, Actuator linkage             CNY                             

9232693  MTP-Plate Holder  2.01                   CNY                             

9232629  Bottle, 500-ml, yellow fitting            CNY                             

9232602  Vacuum manifold, plate holder bottomless              CNY                             

9232579  Cooling block, 1,5ml                   CNY                             

9238951  Locking lever, BS15           CNY                             

9238798  Rectangular bucket, sealable with cap             CNY                             

9238794  Angle rotor 6 x 250 ml               CNY                             

9016913  Tubing, press relief valve inlet DISU v2             CNY                             

9238942  Keyboard-Display assembly, BS15             CNY                             

9238753  Screwdriver, hex2mm, z-drive BR8          CNY                             

9238916  Power supply 40W, BS96          CNY                             

9238748  Coupling, CPC, screw, male, V2                 CNY