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人抗脫氧核糖核蛋白抗體(DNP-Ab)ELISA試劑盒現(xiàn)貨
點(diǎn)擊次數(shù):5發(fā)布時(shí)間:2016/8/13 17:54:22

更新日期:2016/8/13 17:54:22
所 在 地:中國大陸
產(chǎn)品型號(hào):
優(yōu)質(zhì)供應(yīng)
詳細(xì)內(nèi)容
【人抗脫氧核糖核蛋白抗體(DNP-Ab)ELISA試劑盒現(xiàn)貨】規(guī)格:48T/96T
【友情提示】:本產(chǎn)品僅供科研研究使用,不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失,請(qǐng)仔細(xì)閱讀購買說明!
本試劑盒僅供研究使用,齊一生物銷售:021-6034 8496;181214 53965;173021 04490歡迎選購!
操作步驟
1.準(zhǔn)備試劑,樣品和標(biāo)準(zhǔn)品
2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品,37℃反應(yīng)30分鐘
3.洗板5次,加入酶標(biāo)試劑,37℃反應(yīng)30分鐘
4.洗板5次,加入顯色液A、B,37℃顯色15分鐘
5.加入終止液
6.15分鐘之內(nèi)讀OD值
7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性,在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。
【人抗脫氧核糖核蛋白抗體(DNP-Ab)ELISA試劑盒現(xiàn)貨】樣本保存條件:
1.2-8℃冰箱*多放2周
2.-20到-50℃的冰箱可以放3-6個(gè)月
3.-50℃以下的冰箱,可以放一年
【人抗脫氧核糖核蛋白抗體(DNP-Ab)ELISA試劑盒現(xiàn)貨】樣本收集
1.血清樣本用干燥管(紅色)或者促凝管(黃色)收集
2.血漿用抗凝管,一般有EDTA抗凝(紫色)、枸櫞酸鈉抗凝(黑色或者藍(lán)色)
3.動(dòng)物組織勻漿(細(xì)胞):
1g組織加入9ml PBS(濃度為0.1M,pH為7.4的)測(cè)分泌型指標(biāo)
1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)
糞便樣本:1g樣本+9ml PBS(濃度為0.01M,pH為7.4的)
沒有PBS的話也可以用一下生理鹽水,還是用PBS
【人抗脫氧核糖核蛋白抗體(DNP-Ab)ELISA試劑盒現(xiàn)貨】樣本離心轉(zhuǎn)速及時(shí)間
1.試管采集的樣本:3000轉(zhuǎn),離心五分鐘取上清
2.離心管或者EP管采集的樣本:5000--8000轉(zhuǎn)3分鐘取上清
3..樣本保存--如果樣品不立即使用,應(yīng)將其分成小部分-70 ℃保存,避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒,檢測(cè)前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。
【人抗脫氧核糖核蛋白抗體(DNP-Ab)ELISA試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考,如有問題請(qǐng)來電,齊一生物銷售:021-6034 8496;181214 53965;173021 04490
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