【人抗DNA酶B抗體(anti-DNaseB)ELISA試劑盒現(xiàn)貨】ELISA試劑盒常遇見(jiàn)問(wèn)題及解答

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1。我必須運(yùn)行所有的標(biāo)準(zhǔn)和樣品的重復(fù)？

是的，重復(fù)的運(yùn)行，以監(jiān)測(cè)測(cè)定的精度和提高檢測(cè)結(jié)果的信心。

2。我必須在一次運(yùn)行所有的樣品嗎？

不，每個(gè)試劑盒采用stripwell微孔板。這允許用戶在不同的時(shí)間來(lái)分析不同數(shù)量的樣品。

3。什么類型的可重復(fù)性的結(jié)果，得到與分析？

每一個(gè)工具都有一個(gè)包含一個(gè)典型數(shù)據(jù)的圖表的手冊(cè)。在運(yùn)營(yíng)商的任何變化，移液和洗滌技術(shù)，培養(yǎng)時(shí)間、溫度、試劑盒的年齡可以引起變化的結(jié)果。每個(gè)用戶都應(yīng)該得到自己的標(biāo)準(zhǔn)曲線。

4。是否有可能儲(chǔ)存的試劑比表示？

在不推薦使用條件下的部件組件的存儲(chǔ)，以保證測(cè)試的正確性能。

5。我應(yīng)該如何儲(chǔ)存我的樣品？

樣品應(yīng)保存在-20°C或更低的溫度。長(zhǎng)期儲(chǔ)存，建議凍結(jié)他們?cè)?/span>- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進(jìn)行了優(yōu)化，提供*佳的結(jié)果。修改的格式或協(xié)議可能會(huì)給出錯(cuò)誤的結(jié)果。

7。我可以使用一個(gè)不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗(yàn)證在試劑盒插入的樣品類型。未經(jīng)檢驗(yàn)的樣品類型。為進(jìn)一步信息的聯(lián)系技術(shù)服務(wù)。

8。我的樣本產(chǎn)生的值，在動(dòng)態(tài)范圍內(nèi)的測(cè)定。我可以使用這些值嗎？

建議只有在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降的樣本值。標(biāo)準(zhǔn)曲線的范圍內(nèi)的值一般是非線性的，這可能導(dǎo)致不正確的外推值。產(chǎn)生值高于標(biāo)準(zhǔn)的樣品應(yīng)為（進(jìn)一步）稀釋和重復(fù)。如果樣品低于該測(cè)定的范圍內(nèi)，該樣品被認(rèn)為是不可檢測(cè)的。

9。我每次都要運(yùn)行一個(gè)空白或零標(biāo)準(zhǔn)嗎？

是的，這些都是必需的計(jì)算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測(cè)到檢測(cè)。當(dāng)排除特定的檢測(cè)問(wèn)題的來(lái)源時(shí)，它們也非常有幫助。

10。我能改變我在檢測(cè)中使用的樣本量嗎？

不建議你改變卷由于BG工具被設(shè)計(jì)為*佳的性能在給定的體積

11�？梢允褂貌煌慕M件組件嗎？

每一個(gè)組件包含有特定批號(hào)的組件，以確保所有的組件都進(jìn)行優(yōu)化，以及與組件中的所有其他組件。對(duì)這些特定的地段進(jìn)行質(zhì)量檢驗(yàn)。這是從來(lái)沒(méi)有建議使用你自己的組件或組件從其他包或供應(yīng)商。

12。我的標(biāo)準(zhǔn)曲線看起來(lái)很好，但我沒(méi)有得到一個(gè)信號(hào)，我的樣本，當(dāng)我預(yù)期，為什么？

樣品不包含被分析物。一個(gè)矩陣的效果可能是掩蓋檢測(cè)。確保推薦的稀釋液在試劑盒插入后按規(guī)定進(jìn)行。如果建議稀釋，請(qǐng)檢查是否正確進(jìn)行稀釋。過(guò)稀釋會(huì)導(dǎo)致樣品在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降。

13。你怎么推薦我洗盤(pán)子？

如果您使用的是一個(gè)自動(dòng)平板洗衣機(jī)，我們建議定期檢查校準(zhǔn)，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動(dòng)洗衣機(jī)也是一樣的。一個(gè)中繼器或洗瓶也可以使用。用戶應(yīng)小心，以確保所有的內(nèi)容都是送氣和板拍干的無(wú)塵紙。【人抗DNA酶B抗體(anti-DNaseB)ELISA試劑盒現(xiàn)貨】

14。我需要用一個(gè)盤(pán)子嗎？

可靠的結(jié)果可以得到無(wú)板振動(dòng)，但外徑的一般會(huì)低于那些使用平板振動(dòng)器。

15。為什么我要用波長(zhǎng)校正450-570nm之間？

對(duì)于酶聯(lián)免疫吸附試驗(yàn)，在雙波長(zhǎng)讀的是做正確的光密度貢獻(xiàn)的塑料以及，燈和光學(xué)波動(dòng)。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過(guò)程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預(yù)期的分析物濃度，我應(yīng)該找到？

一個(gè)給定的分析物的量可能會(huì)有所不同，不僅從物種的物種，但也組織和細(xì)胞來(lái)源。這個(gè)信息的*佳來(lái)源是目前的文獻(xiàn)，很容易通過(guò)互聯(lián)網(wǎng)在多個(gè)科學(xué)數(shù)據(jù)庫(kù)訪問(wèn)。

18。我的光密度有點(diǎn)高（或較低），比那些在手冊(cè)中，與我的試劑盒來(lái)了。為什么？

光學(xué)密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【人抗DNA酶B抗體(anti-DNaseB)ELISA試劑盒現(xiàn)貨】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【人抗DNA酶B抗體(anti-DNaseB)ELISA試劑盒現(xiàn)貨】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【人抗DNA酶B抗體(anti-DNaseB)ELISA試劑盒現(xiàn)貨】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

QY-L3031 烏苯美司58970-76-6      Ubenimex         58970-76-6       20mg                          

QY-L3032 維生素A68-26-8     Vitamin A         68-26-8    20mg                          

QY-L3033 維生素C50-81-7      Vitamin C          50-81-7    20mg                          

QY-L3034 維生素E13959-02-9        Vitamin E          13959-02-9       20mg                          

QY-L3035 維生素B1268-19-9 Vitamin B12     68-19-9    20mg                          

QY-L3036 維生素K358-27-5   Menadione       58-27-5    20mg                          

QY-L3037 L-纈氨酸72-18-4     L-Valine    72-18-4    20mg                          

QY-L3038 煙酰胺98-92-0        Nicotinamide   98-92-0    20mg                          

QY-L3039 煙酸59-67-6   Nicotinic acid   59-67-6    20mg                           【人抗DNA酶B抗體(anti-DNaseB)ELISA試劑盒現(xiàn)貨】

QY-L3040 L-異亮氨酸73-32-5         L-Isoleucine      73-32-5    20mg                          

QY-L3041 月桂氮卓酮59227-89-3 Laurocapram   59227-89-3       20mg                          

QY-L3042 組氨酸71-00-1        L-Histidine        71-00-1    20mg                          

QY-L3043 植酸鈣3615-82-5   Calcium phytate      3615-82-5         20mg                          

QY-L3044 植酸鈉14306-25-3 Sodium phytate       14306-25-3       20mg                          

QY-L3045 植酸鋅63903-51-5 Zinc Phytate    63903-51-5       20mg                          

QY-L3046 苯乙酸 103-82-2    Phenylacetic acid     103-82-2         20mg                          

QY-L3047 胞嘧啶71-30-7        Cytosine   71-30-7    20mg                          

QY-L3048 尿嘧啶66-22-8        Uracil        66-22-8    20mg                          

QY-L3049 胸腺嘧啶                            20mg                          

QY-L3050 阿卡波糖56180-94-0   Acarbose 56180-94-0       20mg                          

QY-L3051 伏格列波糖                       20mg                          

QY-L3052 羅紅霉素80214-83-1      Roxithromycin 80214-83-1       20mg                          

QY-L3053 阿奇霉素 83905-01-5    Azithromycin   83905-01-5    20mg                          

QY-L3054 雌酚酮53-16-7        "Estrone

"       53-16-7    20mg                          

QY-L3055 次黃嘌呤68-94-0   6-Hydroxypurine      68-94-0    20mg                           【人抗DNA酶B抗體(anti-DNaseB)ELISA試劑盒現(xiàn)貨】

QY-L3056 黃嘌呤69-89-6        2,6-Dihydroxypurine         69-89-6    20mg                          

QY-L3057 鳥(niǎo)嘌呤73-40-5        Guanine   73-40-5    20mg                          

QY-L3058 肌苷58-63-9   Inosine     58-63-9    20mg                          

QY-L3059 腺嘌呤73-24-5        Adenine   73-24-5    20mg                          

QY-L3060 泛酸鈣137-08-6   Calcium pantothenate     137-08-6  20mg                          

QY-L3061 富馬酸 110-17-8  Fumaric acid  110-17-8         20mg                          

9234861  Lid coupling piece for system liquid          CNY                             

9239551  Cbl Assy, XY, LED Driver             CNY                             

9238663  Hook, motorized lid lock           CNY                             

9238642  Capacitor board                 CNY                             

9238626  Opto-Encoder           CNY                             

9238625  Eprom ａnd controller 4K15, Zent3C                  CNY                             

9241828  Pyro Q96 MDa, Premium Agreement      "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9241122  Superlube                   CNY                             

9241007  QIArent&go - Rent out fee QIAcube                 CNY                             

9240985  PCB, drive, cooling device, Qsym              CNY                             

9240982  Sensor, x, sample tracking, Qsym              CNY                             

9240951  Support, cable, portal, Qsym            CNY                             

9234820  Manual, user, QS 4.0, Int                   CNY                             

9240768  Signal Conditioner, Centrifuge, AP96                 CNY                             

9240764  DC Output Module 8NPT, 3AMP, AP96             CNY                             

6000-5003        Duraseal Sealing Film                CNY                              【人抗DNA酶B抗體(anti-DNaseB)ELISA試劑盒現(xiàn)貨】

9241781  RGQ, Full Agreement, no PM  "Repair service delivery: send to Regional Repair Center

Service response time (repair): 7-10 days turnaround time

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: yes, included (provision time 2 days)

Transportation cost coverage: yes, included" CNY                             

9241776  QIAgility, Inspection Service    "The Annual Inspection Service, QIAgility is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAgility are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report."    CNY                             

9241756  IQ/OQ Services, QIAsymphony         Instrument qualification support service for the QIAsymphony. The product contains the Installation Qualification (IQ)/Operation Qualification (OQ) documentation ａnd the performance of the qualification protocols (travel ａnd labor costs included).         CNY                             

9237126  Upgrade App., PCR Setup                  CNY                             

9237093  Training, Extension day    Additional days of training may be purchased during the week of the site visit upon request.         CNY                             

9236426  Board, arm interconnect, TWI                  CNY                             

9236421  Switch, firmware loading, TWI                  CNY                             

9236359  Sheath Filter with Quick Disconnect                  CNY                             

9236346  1.5A Fuse LiquiChip           CNY                             

9236338  Quick disconnect Set                  CNY                             

9236164  Syringe block, repl. kit, RapidPlate           CNY                             

9236153  Locator Plate, Tips, pos 2, BR RP              CNY                             

9235698  Cable 24V, RCS1                 CNY                             

9235296  Capillary, Dispenser head, Mounting Tool                 CNY                             

9235054  Incubator Tower (65°C) Board                   CNY                             

9234941  Incubator Tower (65°C) Board DINCS             CNY                              【人抗DNA酶B抗體(anti-DNaseB)ELISA試劑盒現(xiàn)貨】

9238658  Cooling compressor T2178GK, 50Hz                  CNY