【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說(shuō)明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過(guò)程中必須堅(jiān)持全面質(zhì)量控制和全過(guò)程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【人抗神經(jīng)元核抗體2型/抗Ri抗體(ANNA-2/Ri)ELISA試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人抗神經(jīng)元核抗體2型/抗Ri抗體(ANNA-2/Ri)ELISA試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人抗神經(jīng)元核抗體2型/抗Ri抗體(ANNA-2/Ri)ELISA試劑盒現(xiàn)貨】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過(guò)濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人抗神經(jīng)元核抗體2型/抗Ri抗體(ANNA-2/Ri)ELISA試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問題請(qǐng)來(lái)電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

 QY-25213      Anti-FGF1/FITC熒光素標(biāo)記酸性成纖維細(xì)胞生長(zhǎng)因子           0.5ml/1680                        

 QY-25214      Anti-FoxP3/FITC熒光素標(biāo)記叉頭蛋白3-T細(xì)胞轉(zhuǎn)錄因子抗體IgG               0.5ml/1500                        

 QY-25215      Anti-GFAP/FITC熒光素標(biāo)記膠質(zhì)纖維酸性蛋白抗體IgG               0.5ml/1680                        

 QY-25216      Anti-GFP/FITC (Green Fluorecent protein)熒光素標(biāo)記兔抗綠色熒光蛋白抗體IgG              0.5ml/1500                        

 QY-25217      Anti-GLP-1(7-36)/FITC熒光素標(biāo)記兔抗胰高血糖素樣肽-1抗體IgG            0.5ml/1860                        

 QY-25218      Anti-HSP-27/FITC熒光素標(biāo)記HSP-27蛋白抗體IgG              0.5ml/1500                        

 QY-25219      Anti-HSP-60/FITC熒光素標(biāo)記熱休克蛋白-60蛋白抗體IgG           0.5mg/1500                       

 QY-25220      Anti-HSP-70/FITC熒光素標(biāo)記HSP-70蛋白抗體IgG              0.5ml/1500                        

 QY-25221      Anti-HSP-90α/FITC熒光素標(biāo)記HSP-90α蛋白抗體IgG               0.5ml/1500                        

 QY-25222      Anti-HSP-90β/FITC熒光素標(biāo)記HSP-90β蛋白抗體IgG               0.5ml/1500                        

 QY-25223      Anti-IASPP/FITC熒光素標(biāo)IASPP蛋白抗體IgG              0.5ml/1500                         【人抗神經(jīng)元核抗體2型/抗Ri抗體(ANNA-2/Ri)ELISA試劑盒現(xiàn)貨】

 QY-25224      Anti-IGF-I/FITC熒光素標(biāo)記標(biāo)記胰島素樣生長(zhǎng)因子-I抗體IgG            0.5ml/1500                        

 QY-25225      Anti-Insulin/FITC熒光素標(biāo)記標(biāo)記胰島素抗體IgG           0.5ml/1500                        

 QY-25226      anti-Integrin avb3/FITC熒光素標(biāo)記整合素avb3抗體IgG               0.5ml/1860                        

 QY-25227      Anti-Insulin/HRP辣根過(guò)氧化物酶標(biāo)記胰島素抗體IgG            0.1ml/800                          

 QY-25228      Anti-ISR-α /FITC(Insulin Receptor-α)熒光素標(biāo)記胰島素受體抗體             0.5ml/1500                        

 QY-25229      Anti-ISR-α protein/FITC熒光素標(biāo)記胰島素受體-α蛋白抗體IgG               0.5ml/1500                        

 QY-25230      Anti-ISR-α protein/RBITC羅丹明標(biāo)記胰島素受體-α 抗體IgG           0.5ml/1860                        

 QY-25231      ITG-α/FITC熒光素標(biāo)記整合素α4抗體IgG           0.5ml/1500                        

 QY-25232      Anti-Leptin/FITC熒光素FITC標(biāo)記瘦素抗體IgG             0.5ml/1500                        

 QY-25233      Anti-LPAM-1/FITC（Integrin alpha4,beta7）熒光素標(biāo)記整合素α4β7抗體IgG          0.5ml/1500                        

 QY-25234      Anti-MMP-9/FITC熒光素標(biāo)記基質(zhì)金屬蛋白酶-9蛋白抗體IgG             0.5ml/1500                        

 QY-25235      Anti-MRP2/FITC熒光素標(biāo)記多藥耐藥相關(guān)蛋白2抗體           0.5ml/1500                        

 QY-25236      Anti-NGFR/FITC熒光素標(biāo)記神經(jīng)生長(zhǎng)因子受體抗體gG(標(biāo)記抗體)             0.5ml/1500                        

 QY-25237      MMP-7/FITC熒光素標(biāo)記基質(zhì)金屬蛋白酶-7抗體IgG             0.5ml/1500                        

 QY-25238      Anti-NIK/FITC熒光素標(biāo)記NFkB誘導(dǎo)的激酶抗體IgG            0.5ml/1500                        

 QY-25239      Anti-OVA/HRP辣根過(guò)氧化物酶標(biāo)記兔抗雞卵白蛋白抗體IgG               0.1ml/480                           【人抗神經(jīng)元核抗體2型/抗Ri抗體(ANNA-2/Ri)ELISA試劑盒現(xiàn)貨】

 QY-25240      Anti-PCNA/FITC熒光素標(biāo)記兔抗增值細(xì)胞核抗原抗體IgG           0.5ml/1500                        

 QY-25241      Anti-PKB/FITC熒光素標(biāo)記蛋白激酶B抗體IgG              0.5ml/1500                        

 QY-25242      Anti-P-GP/FITC熒光素標(biāo)記P-糖蛋白抗體IgG         0.5ml/1500                        

 QY-25243      Anti-PTP-1B/FITC熒光素標(biāo)記PTP-1B蛋白抗體IgG              0.5ml/1680                        

 QY-25244      Anti-Survivin/RBITC紅色熒光素羅丹明標(biāo)記 Survivin 蛋白抗體(標(biāo)記抗體)         0.5ml/1680                        

 QY-25245      Anti-Resistin/FITC熒光素標(biāo)記抵抗素抗體IgG         0.5ml/1500                        

218422    Mouse miScript Assay 96 SetVXX.X (20)           CNY                           

9018306  EZ1 Advanced DSP Virus Card            CNY                           

800349    BAC End-Sequencing, w/o prep           CNY                           

9015302  Stopper, reagent tub, right, M48           CNY                           

9015295  Supporters, cap seal, M48 (6)              CNY                           

9015292  O-rings, nozzles, M48 (6)            CNY                           

9016757  Appl. Pack Sequencing for Univ.PF             CNY                           

9015321  Belt, P-motor, M48        CNY                           

133133    NeXtalStock Sodium ac. pH 4.6 (200)         CNY                           

9243231  RCS, Full Agreement     "

- Service response time RCS: 5 working days

- Inspection/maintenance delivery: yes, included; 1 /agreement year

- Cost coverage for repair parts: yes, included

- Cost coverage for labor: yes, included

- Cost coverage for travel: yes, included

- Replacement system provision for DML: yes, included

- Transportation cost coverage for DML: yes, included

For defect units, after customer calling QIAGEN technical service hotline before 12 am:

RCS:

- QIAGEN shall dispatch a specialist to repair the defect instrument at customer site within 48 Hrs / 2 working days.

DML:

- A special package containing a replacement unit shall be sent to the customer within 48 Hrs / 2 working days.

- Customer shall send the defect unit to QIAGEN Regional Repair Center following the instructions attached with the packaging.  

Note: for DML owned by customer, QIAGEN Regional Repair Center will send back the repaired original unit to customers within 7-10 working days.

PC, Monitor, Heater, Shaker, Washer, Printer ａnd Votexer:

- A new unit shall be sent to customer within  7-10 working days at no extra cost

- Customer shall send the defect unit to QIAGEN for disposal

- On-site installation of PC ａnd application software is not covered by the agreement

The Preventive Maintenance (PM) service ensures the correct operation of the equipment. During the PM, all the instrument modules are inspected by a qualified QIAGEN service technician. The Preventive Maintenance is performed under the manufacturer guidelines ａnd documented according to GLP / GMP"  CNY                           

36113      Ni-NTA Magnetic Agarose Beads (6 x 1 ml) 6 x 1 ml nickel-charged magnetic agarose beads (5% suspension)     CNY                           

950207    Reagent Pack, VX  The Reagent Pack, VX, which is used together with the Virus Plasticware Pack, QIAxtractor (#950047) provides fully automated viral nucleic acid purification on the QIAxtractor. The combination of reagents ａnd plasticware enables purification of viral nucleic acids from liquid samples, with a throughput of 8-96 samples per run.  CNY                            【人抗神經(jīng)元核抗體2型/抗Ri抗體(ANNA-2/Ri)ELISA試劑盒現(xiàn)貨】

9018299  EZ1 Advanced DNA Dried Blood Card         CNY                           

9239543  Assy, XY, Slotted Optical Switch 1              CNY                           

9015299  Motor, P/M, M48          CNY                           

9015294  Plate, ｄrop catcher, M48              CNY                           

132981    NeXtal Stock Kit Cryo   24 x 10 ml tubes containing a selection of cryoprotectants  CNY                           

9016755  Appl. Pack Genotyping for Univ.PF             CNY                           

9019453  Front label incl. cutout for  P/R, HS           CNY                           

9019450  IC PIC, programmed , HS            CNY                           

9014276  Positioning plate            CNY                           

9019906  Seal - VPT Filter probes, PyroQ24VW         CNY                           

9019905  Insert VPT, PyroQ24VW             CNY                           

9018155  Sensor, bottle, waste drawer, Qsym            CNY                           

9018147  Consumable drawer, Qsym           CNY                           

9018144  Cable, heated shaker, Qsym          CNY                           

9018090  Cooling adapter, Reagent holder 1, Qsym            CNY                           

9018068  Kit, fuse, Qsym             CNY                            【人抗神經(jīng)元核抗體2型/抗Ri抗體(ANNA-2/Ri)ELISA試劑盒現(xiàn)貨】

9017851  Labware Tray        CNY                           

9017845  Shaker Adapter, 2.0 ml          CNY                           

9017818  Sensor, light barrier, gripper, QIAC              CNY