價(jià)格詳細(xì)資料：試劑盒價(jià)格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認(rèn)同。齊一生物大品牌保證，傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)產(chǎn)品。

【人血管緊張素Ⅱ受體2抗體(AT2R-Ab)ELISA試劑盒現(xiàn)貨】注意事項(xiàng)

1. 當(dāng)混合蛋白溶液時(shí)應(yīng)盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時(shí)間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。

4. 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線，做復(fù)孔。

5. 如標(biāo)本中待測(cè)物質(zhì)含量過高，請(qǐng)先稀釋后再測(cè)定，計(jì)算時(shí)請(qǐng)*后乘以稀釋倍數(shù)。

6. 在配制標(biāo)準(zhǔn)品、檢測(cè)溶液工作液時(shí)，請(qǐng)以相應(yīng)的稀釋液配制，不能混淆。

7. 底物請(qǐng)避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人血管緊張素Ⅱ受體2抗體(AT2R-Ab)ELISA試劑盒現(xiàn)貨】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人血管緊張素Ⅱ受體2抗體(AT2R-Ab)ELISA試劑盒現(xiàn)貨】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人血管緊張素Ⅱ受體2抗體(AT2R-Ab)ELISA試劑盒現(xiàn)貨】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

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QY-15562 Anti-MCP-1 (monocyte chemotactic protein1)巨噬細(xì)胞趨化蛋白-1抗體                                                 

QY-15572 Anti-M-CSF (Macrophage Colony Stimulating Factors)巨噬細(xì)胞克隆刺激因子抗體                                                  

QY-15582 Anti-MDM2 (urine double minute 2)雙微體2癌基因抗體                                                  【人血管緊張素Ⅱ受體2抗體(AT2R-Ab)ELISA試劑盒現(xiàn)貨】

QY-15592 Anti-Megsin/SER—PINB7絲氨酸(或半胱氨酸)蛋白酶抑制劑B7抗體                                                     

QY-15602 Anti-Melan-A/MART-1黑色素瘤相關(guān)抗原/黑色素-A抗體                                                

QY-15612 Anti-Metal ion transporter擬南介金屬離子轉(zhuǎn)運(yùn)蛋白抗體                                              

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QY-15762 Anti-MMP-14(Matrix metalloproteinase-14)基質(zhì)金屬蛋白酶-14抗體                                                       

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211031     QuantiTect Virus +ROX Vial Kit (50)                  CNY                             

9012759  Magnetic Stirrer, 5 position              CNY                             

9012752  Set of Tubing for Olympus PK80                 CNY                             

9010331  Cover , NTP-Reader, Plato 1/3, (9194)               CNY                             

9010085  Modul, SamTrak, 12 Racks, P3xxx             CNY                             

979201     PyroMark Q24 Plate (100)        For sample analysis of template DNA on Q24 CNY                             

979008     PyroMark Wash Buffer (conc., 200 ml)    For washing ａnd neutralizing the immobilized DNA.       CNY                             

311714     BioMag Streptavidin (50ml)     BioMag streptavidin suspension (5 mg/ml)      CNY                             

9002273  PyroMark Q24 Advanced, PrioPLUS                   CNY                             

9002200  QIAxcel Advanced, PrioPLUS, no PM        "QIAxcel Advanced Instrument

Installation of QIAxcel Advanced & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10

Loaner available in 2 days*

No Preventive Maintenance included

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                              【人血管緊張素Ⅱ受體2抗體(AT2R-Ab)ELISA試劑盒現(xiàn)貨】

206662     Type-it CNV Probe PCR +qC Kit (100)                 CNY                             

206402     QIAGEN LongRange PCR Kit (100)   For 100 PCR rxns a 50 µl: Enzyme Mix (Taq ａnd Proofreading Enzyme, (5 u/µl), 10 x PCR Buffer for increased fidelity (contains dNTP´s), 25 mM MgCl2, 5 x Q-Solution         CNY                             

9012730  Cable, flat band, X, 2300 mm           CNY                             

979003     PyroMark Q96 Sample Prep Tool Cover (10)    Consumables for use with magnetic tool for isolation of single stranded DNA.         CNY                             

978746     PyroMark CpG Assay (200)                CNY                             

9012702  Motor, Y-drive, BCR, P1/3/7                CNY                             

929702     QX Buffer Tray          CNY                             

330421     RT² Easy First Strand Kit (12)            CNY                             

9010540  Tischplatte Plato-7 (9194)                 CNY                             

68163       DNeasy Plant Maxi Kit (24)      24 DNeasy Maxi Spin Columns, 24 QIAshredder Maxi Spin Columns, RNase A, Buffers, Collection Tubes (50 ml)   CNY                             

9012781  Upgrade Y2K from Rufas 3.4 to Winrufas                  CNY                             

9010051  Plate stacker             CNY                             

60704       QIAamp DSP Virus Kit (50),CE          CNY                             

59935       #EpiTect MethyLight Assay Hs-TMEFF2(100)  "10-fold primer TaqMan probe mix, for real-time MethyLight PCR

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301807     HiPerFect HTS Reagent (6 x 1 ml)             CNY                             

301707     HiPerFect Transfection Reagent (4x1 ml)                  CNY                              【人血管緊張素Ⅱ受體2抗體(AT2R-Ab)ELISA試劑盒現(xiàn)貨】

59760       EpiTect Fast 96 LyseAll Bisulfite Kit           CNY                            

206624     Type-it CNV SYBR Green PCR Core Kit(400)              CNY                             

9012739  Peristaltic Pump outlet tubing 1.25                   CNY                             

133242     NeXtalStock Sodium malonate pH 4.0 (200)             CNY                             

133235     NeXtalStock Sodium citrate pH 5.0 (200)                  CNY