檢測(cè)標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測(cè)方法：ELISA

檢測(cè)類(lèi)型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠(chéng)為您服務(wù)！本試劑盒用于測(cè)定血清,血漿及相關(guān)液體樣本中含量.

【人粒細(xì)胞巨噬細(xì)胞集落刺激因子抗體(GM-CSFAb)ELISA試劑盒現(xiàn)貨】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值）,通過(guò)標(biāo)準(zhǔn)曲線(xiàn)計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

【人粒細(xì)胞巨噬細(xì)胞集落刺激因子抗體(GM-CSFAb)ELISA試劑盒現(xiàn)貨】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過(guò)氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【人粒細(xì)胞巨噬細(xì)胞集落刺激因子抗體(GM-CSFAb)ELISA試劑盒現(xiàn)貨】

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測(cè)樣品孔.在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿(mǎn)洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測(cè)定：以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）. 測(cè)定應(yīng)在加終止液后15分鐘以?xún)?nèi)進(jìn)行.

注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開(kāi)封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果. 【人粒細(xì)胞巨噬細(xì)胞集落刺激因子抗體(GM-CSFAb)ELISA試劑盒現(xiàn)貨】

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線(xiàn),做復(fù)孔.如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說(shuō)明書(shū)的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說(shuō)明書(shū)有異,以英文說(shuō)明書(shū)為準(zhǔn).

計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線(xiàn),根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線(xiàn)查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線(xiàn)的直線(xiàn)回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線(xiàn)性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見(jiàn)應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

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QY-14812 Anti-IL-23(Interleukin-23)白介素-23抗體                                                    

QY-14822 Anti-IL-4(Interleukin-4)白介素4抗體                                                  

QY-14832 Anti-IL-6 (Interleukin-6) Human白介素6                                                     

QY-14842 Anti-IL-6 (Interleukin-6) mouse、rat白介素6抗體                                                    

QY-14852 Anti-IL-6(Interleukin-6/interferon, beta 2)mouse rat白介素6抗體                                                    

QY-14862 Anti-IL-8（Interleukin-8）rat mouse白介素8抗體                                                   

QY-14872 Anti-ILK-1 (Integrin-linked protein kinase 1)整合素連接激酶-1抗體                                                  

QY-14882 anti-ING1/p33(inhibitor of growth gene 1)生長(zhǎng)抑制因子基因1抗體                                                

QY-14892 Anti-Inhibin α抑制素α抗體                                                      

QY-14902 Anti-Insulin胰島素抗體                                               

QY-14912 Anti-Insulin Receptor-α（ISR-α）胰島素受體-α抗體                                                    

QY-14922 Anti-Insulin(1-51 protein)胰島素1-51蛋白抗體                                                 

QY-14932 Anti-Integrin α3β1整合素α3β1抗體                                                  

QY-14942 Anti-Integrin α4β7/LPAM-1 (Lymphocyte Peyer’s patch Adhesion Molecule 1/Integrin alpha4,beta7)整合素α4β7抗體                                                        【人粒細(xì)胞巨噬細(xì)胞集落刺激因子抗體(GM-CSFAb)ELISA試劑盒現(xiàn)貨】

QY-14952 anti-Integrin avb3整合素avb3抗體                                                   

QY-14962 Anti-Integrin αE2/CD103整合素αE2抗體                                                       

QY-14972 Anti-IQGAP1支架蛋白抗體                                                 

QY-14982 Anti-IRS P53 (Insulin receptor suRCtrate P53)胰島素受體底物p53蛋白抗體                                                   

QY-14992 Anti-IRS-1胰島素受體底物-1抗體                                                      

QY-15002 Anti-IRS-2胰島素受體底物-2抗體                                                      

QY-15012 Anti-IRS-3胰島素受體底物-3抗體                                                      

QY-15022 Anti-IRS-4胰島素受體底物-4抗體                                                      

QY-15032 Anti-ISR（Insulin Receptor）胰島素受體抗體                                                    

QY-15042 Anti-ISR-α （Insulin Receptor-α）胰島素受體-α抗體                                                  

QY-15052 Anti-ISR-β （Insulin Receptor-β）胰島素受體-β抗體                                                  

QY-15062 Anti-ITG-α/ CD49d整合素α4抗體                                                   

QY-15072 Anti-ITG-β1/CD29（integrin β1）粘附分子整合素β1抗體                                                 

QY-15082 Anti-ITG-α5/CD49e（Integrin α5）粘附分子整合素α5抗體                                                       

QY-15092 Anti-ITG-β3/CD61(Integrin β chain,β3 precursor)整合素-β3抗體                                                      

QY-15102 Anti-JNK1/3 (c-Jun amino-terminal kinase 1/3)c-Jun氨基末端激酶1/3抗體                                                  【人粒細(xì)胞巨噬細(xì)胞集落刺激因子抗體(GM-CSFAb)ELISA試劑盒現(xiàn)貨】

QY-15112 Anti-KATPC (ATP-Sensitive K+ Channel Subunits uKATP-1—ATP)ATP敏感性鉀通道蛋白抗體                                                     

QY-15122 Anti-Ki-67Ki-67 Antigen抗體                                               

9011433  BGR Z-Motor P1300 Spez. MWG              CNY                             

9011422  Tip Rack Holder, RCS1               CNY                             

9243119  Green Laser Contr. Coherent 115M-13, LC               CNY                             

9243556  HC2, Full Agreement        "Service response time (on-site repair):not applicable

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage depot repair (parts, labor, packaging, shipping): yes, full coverage

For defect DML, after customer calling QIAGEN technical service hotline before 12 am:

- A special package containing a replacement unit shall be sent to the customer within 2 working days.

- Customer shall send the defect unit to QIAGEN Regional Repair Center following the instructions attached with the packaging.  

For DML owned by customer, QIAGEN Regional Repair Center will send back the repaired original unit to customers within 7-10 working days.

PC, Monitor, Heater, Shaker, Washer, Printer ａnd Votexer:

- A new unit shall be sent to customer within  7-10 working days at no extra cost

- Customer shall send the defect unit to QIAGEN for disposal

- On-site installation of PC ａnd application software is not covered by the agreement

The Preventive Maintenance (PM) service ensures the correct operation of the equipment. During the PM, all the instrument modules are inspected by a qualified QIAGEN service technician. The Preventive Maintenance is performed under the manufacturer guidelines ａnd documented according to GLP / GMP"    CNY                             

101.901-24       #HLA-DQB1*0602; DQA1*0102 incl.Taq (24)           CNY                             

9241033  #Tube insert, 11 mm, sample carr            CNY                             

9242760  #Warranty PLUS 1, Prem/RRL, 1PM,QSYM RGQ              CNY                             

677491     ipsogen NPM1 mut A gDNA MutaQuant Stds          CNY                              【人粒細(xì)胞巨噬細(xì)胞集落刺激因子抗體(GM-CSFAb)ELISA試劑盒現(xiàn)貨】

9011164  Roller, deflection, X-drive, BR8000           CNY                             

9010980  BGR 1-er Shaker PK80 (1177)           CNY                             

133215     NeXtalStock MMT pH 9.0 (200)                 CNY                             

301005     Attractene Transfection Reagent (1 ml)           CNY                             

9012675  Peristaltic Pump, 4 channel (A04897)               CNY                             

9012671  Cable, PCON98 to release valve, BR8000                  CNY                             

9002029  Rotor-Gene Q MDx 5plex, PrioPLUS         "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

Warranty extension to 3 yrs

Regional Repair Center service, 7-10 day turn around time

Loaner available in 2 days*

Annual Inspections (1PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

9002019  Rotor-Gene Q MDx 2plex HRM, PrioPLUS       "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

Warranty extension to 3 yrs

Loaner available in 2 days*

Annual Inspections (1 onsite PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

9002002  Rotor-Gene Q MDx 2plex Platform          CNY                             

970540     UGT1A1 Pyro Kit (24)                CNY                             

293025     mericon Corn (96)             CNY                             

293015     mericon Soy Kit (96)                   CNY                             

929525     QX Alignment Marker 50bp/500bp (1.5 ml)              CNY                             

206241     Type-it Microsatellite PCR Kit (70)   "The Type-it Microsatellite PCR Kit is developed for fast ａnd reliable genotypic analysis of humans, animals, plants, ａnd bacteria using

microsatellites, STR, or VNTR markers without the need for lengthy optimization procedures

Features

 Reliable microsatellite analysis by multiplex PCR

 Microsatellite assay development without optimization

 Successful ａnd specific coamplification of all fragments

 Optimized protocols for any detection platform"       CNY                             

291524     mericon Quant MON 810 (48)          CNY                             

9001904  QIAgility HEPA/UV (PC) PrioPLUS     "QIAgility HEPA/UV (PC)Instrument

Installation of QIAgility instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                              【人粒細(xì)胞巨噬細(xì)胞集落刺激因子抗體(GM-CSFAb)ELISA試劑盒現(xiàn)貨】

9001899  QIAxtractor, UV/HEPA (PC) Priority QIAxtractor, UV/HEPA (PC) Priority includes the QIAxtractor instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service ａnd inspection is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.        CNY                             

133124     NeXtalStock TRIS pH 8.5 (200)                   CNY                             

965942     QIAamp Investigator BioRobot Kit (12)            CNY                             

133191     NeXtalStock D-(+)-Sucrose (200)               CNY                             

133184     NeXtalStock Citric acid pH 3.6 (200)                  CNY                             

9018472  Set, O-rings (10), QIAC              CNY                             

133101     NeXtalStock Potassium sulfate (200)                 CNY