齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1。我必須運行所有的標準和樣品的重復？

是的，重復的運行，以監(jiān)測測定的精度和提高檢測結果的信心。

2。我必須在一次運行所有的樣品嗎？

不，每個試劑盒采用stripwell微孔板。這允許用戶在不同的時間來分析不同數(shù)量的樣品。

3。什么類型的可重復性的結果，得到與分析？

每一個工具都有一個包含一個典型數(shù)據(jù)的圖表的手冊。在運營商的任何變化，移液和洗滌技術，培養(yǎng)時間、溫度、試劑盒的年齡可以引起變化的結果。每個用戶都應該得到自己的標準曲線。

4。是否有可能儲存的試劑比表示？

在不推薦使用條件下的部件組件的存儲，以保證測試的正確性能。

5。我應該如何儲存我的樣品？

樣品應保存在-20°C或更低的溫度。長期儲存，建議凍結他們在- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進行了優(yōu)化，提供*佳的結果。修改的格式或協(xié)議可能會給出錯誤的結果。

7。我可以使用一個不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗證在試劑盒插入的樣品類型。未經(jīng)檢驗的樣品類型。為進一步信息的聯(lián)系技術服務。

8。我的樣本產生的值，在動態(tài)范圍內的測定。我可以使用這些值嗎？

建議只有在標準曲線的范圍內下降的樣本值。標準曲線的范圍內的值一般是非線性的，這可能導致不正確的外推值。產生值高于標準的樣品應為（進一步）稀釋和重復。如果樣品低于該測定的范圍內，該樣品被認為是不可檢測的。

9。我每次都要運行一個空白或零標準嗎？

是的，這些都是必需的計算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測到檢測。當排除特定的檢測問題的來源時，它們也非常有幫助。

10。我能改變我在檢測中使用的樣本量嗎？

不建議你改變卷由于BG工具被設計為*佳的性能在給定的體積

11。可以使用不同的組件組件嗎？

每一個組件包含有特定批號的組件，以確保所有的組件都進行優(yōu)化，以及與組件中的所有其他組件。對這些特定的地段進行質量檢驗。這是從來沒有建議使用你自己的組件或組件從其他包或供應商。

12。我的標準曲線看起來很好，但我沒有得到一個信號，我的樣本，當我預期，為什么？

樣品不包含被分析物。一個矩陣的效果可能是掩蓋檢測。確保推薦的稀釋液在試劑盒插入后按規(guī)定進行。如果建議稀釋，請檢查是否正確進行稀釋。過稀釋會導致樣品在標準曲線的范圍內下降。

13。你怎么推薦我洗盤子？

如果您使用的是一個自動平板洗衣機，我們建議定期檢查校準，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動洗衣機也是一樣的。一個中繼器或洗瓶也可以使用。用戶應小心，以確保所有的內容都是送氣和板拍干的無塵紙。【人層連蛋白/板層素(LN)ELISA試劑盒廠家】

14。我需要用一個盤子嗎？

可靠的結果可以得到無板振動，但外徑的一般會低于那些使用平板振動器。

15。為什么我要用波長校正450-570nm之間？

對于酶聯(lián)免疫吸附試驗，在雙波長讀的是做正確的光密度貢獻的塑料以及，燈和光學波動。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預期的分析物濃度，我應該找到？

一個給定的分析物的量可能會有所不同，不僅從物種的物種，但也組織和細胞來源。這個信息的*佳來源是目前的文獻，很容易通過互聯(lián)網(wǎng)在多個科學數(shù)據(jù)庫訪問。

18。我的光密度有點高（或較低），比那些在手冊中，與我的試劑盒來了。為什么？

光學密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【人層連蛋白/板層素(LN)ELISA試劑盒廠家】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【人層連蛋白/板層素(LN)ELISA試劑盒廠家】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【人層連蛋白/板層素(LN)ELISA試劑盒廠家】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

QY-13152 Anti-FOSL2 (FOS-like antigen-2)FOSL2抗體                                                  

QY-13162 anti-FoxP1（Forkhead box P1）FoxP1（T細胞轉錄因子）抗體                                              

QY-13172 Anti-FoxP3叉頭蛋白3-T細胞轉錄因子                                                      

QY-13182 anti-Measles virus of fusion protein抗麻疹病毒抗體（麻疹病毒融合蛋白）                                                   【人層連蛋白/板層素(LN)ELISA試劑盒廠家】

QY-13192 Anti-FSH (Anti-Human Follicle Stimulating Hormone Monoclonal Antibody）鼠抗人促卵泡生成素單克隆抗體                                                      

QY-13202 anti-FSH-R(Follicle-stimulating hormone receptor precursor)促卵泡刺激素受體抗體                                                       

QY-13212 Anti-G Protein(Guanine nucleotide-binding regulatory protein)G蛋白/鳥苷酸結合蛋白抗體                                                  

QY-13222 Anti-GABA-ARα1γ1氨基丁酸受體α1抗體                                                    

QY-13232 anti-GABAB1(GABAb Receptor 1)抗g-氨基丁酸B1受體抗體                                                   

QY-13242 anti-GABAB2(GABAb Receptor 2)抗g-氨基丁酸B2受體抗體                                                   

QY-13252 Anti-GAD-65 (glutamate decarboxylase)谷氨酸脫羧酶-65抗體                                                

QY-13262 Anti-GAD-67 (glutamate decarboxylase 67)谷氨酸脫羧酶-67抗體                                                    

QY-13272 Anti-GAD-65 (glutamate decarboxylase)谷氨酸脫羧酶-65抗體(C端多肽)                                               

QY-13282 Anti-Galanin神經(jīng)節(jié)肽抗體“甘丙肽”                                                      

QY-13292 Anti-Galectin-3半乳糖凝集素-3抗體                                                 

QY-13302 Anti-GAP-43 ( Growth associated protein-43 )神經(jīng)生長相關蛋白-43（抗體）                                                 

QY-13312 Anti-GAPDH （Ribbt Anti-glyceraldehyde-3-phosphate Dehydrogease ）3-磷酸甘油醛脫氫酶                                                     

QY-13322 Anti-GAPDH （Ribbt Anti-glyceraldehyde-3-phosphate Dehydrogease ）humanAnti-GAPDH （Ribbt Anti-glyceraldehyde-3-phosphate Dehydrogease ）human3-磷酸甘油醛脫氫酶(人)                                                       

QY-13332 Anti-GAPDH （Ribbt Anti-glyceraldehyde-3-phosphate Dehydrogease ）rat、mouse3-磷酸甘油醛脫氫酶（大鼠、小鼠）                                                       

QY-13342 Anti-GAPDH（Mouse Anti-glyceraldehyde-3-phosphate Dehydrogease ） (暫無現(xiàn)貨)3-磷酸甘油醛脫氫酶（小鼠來源） (暫無現(xiàn)貨)                                                     【人層連蛋白/板層素(LN)ELISA試劑盒廠家】

QY-13352 Anti-GAPDH（Ribbt Anti-glyceraldehyde-3-phosphate Dehydrogease ）3-磷酸甘油醛脫氫酶（兔來源免疫組化用抗體）                                                       

QY-13362 Anti-GASR(Gastrin receptor)G蛋白偶聯(lián)受體-2抗體                                                 

QY-13372 Anti-Gastrin（Bombesin)胃泌素/蛙皮素抗體                                                     

QY-13382 Anti-Gastrin（Bombesin)胃泌素/蛙皮素                                                     

QY-13392 Anti-Gax（growth arrest-specific homeobox）生長終止特異性同源盒基因抗體                                                    

QY-13402 Anti-GCS (glucosylceramide synthase, GCS)谷氨酸半胱氨酸γ合成酶抗體                                                       

QY-13412 anti-G-CSF(Granulocyte Colony Stimulating Factors)粒細胞-巨噬細胞集落刺激因子抗體                                               

QY-13422 anti-GDF-8（growth differntiation，factor8）肌細胞生長抑制素抗體                                                    

QY-13432 Anti-GDNF (Glial cell line-derived neurotrphic factor)膠質細胞源性神經(jīng)營養(yǎng)因子抗體                                                   

QY-13442 Anti-Gelsolin凝溶膠蛋白抗體                                                      

QY-13452 Anti-GFAP（Glial Fibrillary Acidic Protein）膠質纖維酸性蛋白抗體                                                 

QY-13462 Anti-GFP (Green Fluorecent protein)綠色熒光蛋白（免疫組化用抗體）                                                

QY-13472 Anti-GFP (Green Fluorecent protein)綠色熒光蛋白                                                     

301525     TransMessenger Tranfect. Reagent(0.5ml)     For 60 transfections in 6-well plates or 80 transfections in 12-well plates         CNY                             

52304       QIAamp RNA Blood Mini Kit (50)     For 50 RNA preps: 50 QIAamp Mini Spin Columns, 50 QIAshredder Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd Buffers    CNY                             

51306       QIAamp DNA Mini Kit (250)     For 250 DNA preps: 250 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)     CNY                              【人層連蛋白/板層素(LN)ELISA試劑盒廠家】

9002012  Rotor-Gene Q MDx 2plex HRM Platform                  CNY                             

970530     NRAS Pyro Kit (24)            CNY                             

970022     PyroMark Q24 CpG MLH1 (4 x 24)   For quantification of methylation level in region -209 to -188 from the transcription start site. CNY                             

969261     Ni-NTA Superflow 96 BioRobot Kit (4)               CNY                             

133199     NeXtalStock HEPES pH 8.0 (200)               CNY                             

292143     mericon Horse Kit (24)              CNY                             

206047     Type-it Fast SNP Probe PCR Kit (4000)     "Features

 Outstanding separation for reliably high call rates

 For difficult SNP loci ａnd for low amounts of template

 Validated using TaqMan SNP Genotyping Assays

 Up to 40% time savings due to fast-cycling procedure

 Convenient ｍaster mix format ａnd optimized protocol"   CNY                             

292025     mericon Cattle (96)           CNY                             

291113     mericon RR Soy Kit (24)            CNY                             

9001908  QIAgility HEPA / UV (w/o PC) System       QIAgility HEPA / UV (w/o PC) System combines the QIAgility instrument ａnd installation. The Installation plus Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document. CNY                             

133111     NeXtalStock Sodium fluoride (200)           CNY                             

9018540  Kit, installation, Qsym               CNY                             

9240672  Kerk Nut, Common, AP             CNY                             

9240658  Pump, Vacuum, Jun-Air, AP               CNY                             

9240642  Cable Assy, Proteinase K Pump, AP196/98                  CNY                             

9240638  Opto Sensor, Reagent Pump, AP               CNY                              【人層連蛋白/板層素(LN)ELISA試劑盒廠家】

9240617  Fuse, 2.0A, FNM, AP96             CNY                             

9240589  MAC Valve, 4-Way, Cent. Locator, AP96           CNY                             

9240560  Rotor/Buckets, Eppendorf, AP196/98                CNY                             

9240554  Bucket Grease, Eppendorf, AP196/98                CNY                             

9240523  Cable, Motor, Axis #0, Dispense, AP98             CNY                             

9240507  Software & Std. Protocols, AP196            CNY                             

9240377  QIAcube, Installation       The Installation, QIAcube facilitates the quick implementation of the QIACube into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the QIAcube instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.  CNY                             

9239571  BR Universal, Basic Agreement       The Basic Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9239570  BR Universal, Premium Agreement         The Premium Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).    CNY                             

9020032  FOOTSET, RGQ                   CNY                             

9019986  PCB, small breakout, 12 core, C12            CNY                             

9019479  UPPER BUFFER LID GS              CNY                             

59203     EpiTect Whole Bisulfitome Kit (25)              CNY                           

59164     EpiTect Plus LyseAll Bisulfite Kit (48)          CNY                           

929526    QX Alignment Marker 50 bp/1 kb (1.5 ml)          CNY                           

133218    NeXtalStock MOPS pH 8.0 (200)        CNY                           

301605    RNAiFect Transfection Reagent (1ml)  RNAiFect Reagent ａnd buffer, for up to 170 transfections in 24-well plates; up to 500 transfections in 96-well plates CNY