【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【人α1酸性糖蛋白(α1-AGP)ELISA試劑盒廠家】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人α1酸性糖蛋白(α1-AGP)ELISA試劑盒廠家】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人α1酸性糖蛋白(α1-AGP)ELISA試劑盒廠家】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人α1酸性糖蛋白(α1-AGP)ELISA試劑盒廠家】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

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QY-09872       anti-apoD (Apolipoprotein/APOD protein )載脂蛋白D抗體                                        

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QY-09972       anti-ARIP2a（Activin receptor 2A）激活素受體2A/激活素受體相互作用蛋白2a抗體                                   

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QY-10072       Anti-ATF1 (Activating Transcription Factor1)活化復(fù)制因子1抗體                                    

QY-10082       Anti-ATF2 (Activating Transcription Factor2)活化復(fù)制因子2抗體                                    

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QY-10152       Anti-BADH2 (Betaine-aldehyde dehydrogenase )BADH2抗體                                       

QY-10162       Anti-Bassoon/RCN(zinc finger protein)鋅指蛋白231抗體                                   

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1012508  Penta.His Antibody 96 Plates (100)              CNY                           

997024    Filter-Tips, 1500 µl (1024)           CNY                           

9018895  Rotor-Disc 100 Rotor           CNY                           

9018569  Magnet unit, w reflector, EZ1a             CNY                           

9013155  Calibration pyramid, v1.02            CNY                           

9013074  Magnet, 96-well, Type C              CNY                           

673113    ipsogen JAK2 MutaScreen RS Kit (19)        CNY                           

673223    ipsogen JAK2 MutaScreen EZ Kit (24) CE          CNY                           

672691    ipsogen RARA-PML bcr3 Standards            CNY                            【人α1酸性糖蛋白(α1-AGP)ELISA試劑盒廠家】

670115    ipsogen BCR-ABL1 Mbcr Kit (52)        CNY                           

380317    Investigator Triplex AFSQS Kit (400)          CNY                           

9001527  PyroMark Q96 MD Automated     For performing automated pyrosequncing reaction on the Q96 MD    CNY                           

9001301  QIAsymphony AS         CNY                           

4883004  pg AIV-H5 RT-PCR ASSAY LE            CNY                           

4826024  careHIV-1 RT-PCR ASSAY V2             CNY                           

4843014  pg HPV PCR ASSAY            CNY                           

130901    NeXtal DWBlock Classics Suite           CNY                           

130724    NeXtal Tubes JCSG Core I Suite         CNY                           

9013221  Holder, tube, STS, single, 5 ml (60), 0         CNY                           

9013206  Fuse set, BR8000          CNY                           

132630    NeXtal Stock Zinc acetate (50)     1M Zinc acetate dihydrate - 50mL (4 x 12,5 ml tube)  CNY                           

765112    PAXgene Tissue Container (10), G              CNY                           

762331    PAXgene 96 Blood RNA Kit (4)     4 PAXgene 96 RNA Plates, 4 PAXgene 96 Filter Plates, Buffers, Proteinase K, RNase-free DNase Sets, AirPore Tape Sheets, Collection Vessels. To be used in conjunction with PAXgene Blood RNA Tubes.       CNY                           

761133    PAXgene Blood DNA Kit (25)       Processing tubes ａnd buffers for 25 preparations CNY                           

203607    HotStarTaq Plus DNA Polymerase (5000)    1 x 5000 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2 CNY                            【人α1酸性糖蛋白(α1-AGP)ELISA試劑盒廠家】

203203    HotStarTaq DNA Polymerase (250)      - 250 units HotStarTaq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2     CNY                           

200403    TopTaq Master Mix Kit (250)       2x TopTaq Master Mix containing 250 units of TopTaq DNA Polymerase in total, 10x CoralLoad Concentrate, ａnd RNase-Free Water; reagents provided for 200 x 50 μl reactions   CNY                           

159994    Generation DNA Elution Soln 2 (500 ml)             CNY                           

158932    Clotspin Baskets (50)            CNY                           

158867    Gentra Puregene Buccal Cell Kit (400)         CNY                           

158467    Gentra Puregene Blood Kit (120 ml)            CNY