檢測標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠為您服務(wù)！本試劑盒用于測定血清,血漿及相關(guān)液體樣本中含量.

【人肝素輔因子Ⅱ(HCⅡ)ELISA試劑盒廠家】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長下測定吸光度（OD值）,通過標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

【人肝素輔因子Ⅱ(HCⅡ)ELISA試劑盒廠家】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測樣品孔.在酶標(biāo)包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測定：以空白空調(diào)零,450nm波長依序測量各孔的吸光度（OD值）. 測定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

【人肝素輔因子Ⅱ(HCⅡ)ELISA試劑盒廠家】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說明書有異,以英文說明書為準(zhǔn).

【人肝素輔因子Ⅱ(HCⅡ)ELISA試劑盒廠家】計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

QT-00100 銅綠假單胞桿菌（綠膿桿菌）ATCC9027     凍干粉                                         

QT-00102 枯草芽孢桿菌黑色變種ATCC9372        凍干粉                                         

QT-00103 艱難梭菌ClostridiumdifficileATCC9689  凍干粉                                         

QT-00104 啤酒酵母ATCC9763        凍干粉                                         

QT-00105 黑曲霉ATCC10582 凍干粉                                         

QT-00106 磚紅鏈霉菌ATCC19776 凍干粉                                         

QT-00107 白色葡萄球菌ATCC8799        凍干粉                                         

QT-00108 皺褶青霉ATCC10128      凍干粉                                         

QT-00109 黑曲霉ATCC10864 凍干粉                                         

QT-00110 釀酒酵母ATCC26603      凍干粉                                         

QT-00111 釀酒酵母ATCC26785      凍干粉                                         

QT-00112 運(yùn)動(dòng)發(fā)酵單孢菌ATCC29191 凍干粉                                         

QT-00113 假單胞桿菌ATCC31554 凍干粉                                         

QT-00114 糞產(chǎn)堿桿菌ATCC31555 凍干粉                                         

QT-00115 菅囊酵母ATCC32691      凍干粉                                         

QT-00116 弗氏檸檬酸桿菌ATCC10787 凍干粉                                          【人肝素輔因子Ⅱ(HCⅡ)ELISA試劑盒廠家】

QT-00117 莢膜紅細(xì)菌ATCC11166 凍干粉                                         

QT-00118 發(fā)根土壤桿菌（發(fā)根植物單胞菌）ATCC11325  凍干粉                                         

QT-00119 生孢梭菌（產(chǎn)芽孢梭狀芽孢桿菌）ATCC11437  凍干粉                                         

QT-00120 痤瘡丙酸桿菌ATCC11827      凍干粉                                         

QT-00121 地衣芽孢桿菌ATCC11946      凍干粉                                         

QT-00122 谷氨酸棒桿菌ATCC13032      凍干粉                                         

QT-00123 熏衣草灰鏈霉菌ATCC13306 凍干粉                                         

QT-00124 豬霍亂沙門氏菌豬霍亂亞種ATCC13312      凍干粉                                         

QT-00125 脫氮假單胞菌ATCC13867      凍干粉                                         

QT-00126 耐放射異常球菌ATCC13939 凍干粉                                         

QT-00127 綠針假單胞菌ATCC13985      凍干粉                                         

QT-00128 腸炎沙門氏菌腸炎亞種ATCC14028      凍干粉                                         

QT-00129 巨大芽孢桿菌ATCC14581      凍干粉                                         

QT-00130 吸水鏈霉菌奧薩霉素亞種ATCC15420 凍干粉                                         

QT-00131 長雙歧桿菌ATCC15707 凍干粉                                         

QT-00132 放射性根瘤菌ATCC15955      凍干粉                                          【人肝素輔因子Ⅱ(HCⅡ)ELISA試劑盒廠家】

QT-00133 短密青霉ATCC16024      凍干粉                                         

9236492  Centrifuge 4-16KS, Full Agreement, no PM     "Repair service delivery: onsite

Service response time (repair): 48 hours/2 business days

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: not applicable"        CNY                             

9236485  Full Agreement, Plato 3, 1 yr   The Full Agreement Plato 3 provides full service coverage for the Plato 3 workstation. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).         CNY                             

9013063  Plate Stacker, detachable, BR8000          CNY                             

9013004  Gripper set, T-handler               CNY                             

672291     ipsogen PML-RARA bcr2 Standards          CNY                             

670724     ipsogen BCR-ABL1 Mbcr IS-MMR Kit (30) CE            CNY                             

386123     Matrix Standard BT5 multi cap. (25)                 CNY                              【人肝素輔因子Ⅱ(HCⅡ)ELISA試劑盒廠家】

384013     Investigator DIPplex Kit (25)             CNY                             

9001510  Hybrid Capture System 2 (non-US)            CNY                             

9001272  TissueRuptor (230V, 50-60 Hz, EU/CH)             CNY                             

4877004  pg AIV-H9 RT-PCR ASSAY           CNY                             

130916     NeXtal DWBlock PEGs II Suite          CNY                             

4601263  artus Malaria RG PCR Kit (24) CE              CNY                             

4801024  careHBV PCR ASSAY                   CNY                             

4811024  careHCV RT-PCR ASSAY V2                CNY                             

4831024  careTB PCR ASSAY             CNY                             

4837014  careNG PCR ASSAY            CNY                             

4838024  careNG PCR ASSAY V2               CNY                             

4851014  careHCMV PCR ASSAY               CNY                             

4854024  careTP PCR ASSAY II                   CNY                             

4862004  PG NDV Univ. RT-PCR Kit (48) LE Z            CNY                             

130824     NeXtal CubicPhase 2w Mo/C 8% µplate (10)            CNY                             

4518363  artus HCV QS-RGQ Kit (24) CE          CNY                              【人肝素輔因子Ⅱ(HCⅡ)ELISA試劑盒廠家】

4552165  artus C. trachomatis TM PCR Kit (96) CE          CNY                             

130711     NeXtal Tubes MBClass Suite              CNY                             

130706     NeXtal Tubes MPD Suite           CNY                             

122117     SeqTarget LongRange PCR Kit (500)                  CNY                             

84020       Vacuum Pump (230V, 50Hz)              CNY                             

75842       RNeasy Lipid Tissue Midi Kit (10)     10 RNeasy Midi Spin Columns, Collection Tubes (15 ml), QIAzol Lysis Reagent, RNase-free Reagents ａnd Buffers       CNY                             

75144       RNeasy Midi Kit (50)         50 RNeasy Midi Spin Columns, Collection Tubes (15 ml), RNase-free Reagents ａnd Buffers   CNY                             

28006       MinElute PCR Purification Kit (250) 250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)  CNY