ELISA 檢測的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

注意事項(xiàng)：

◆   每個(gè)標(biāo)本量收集體積＝  100ul × 檢測種類，如要做復(fù)孔，標(biāo)本量收集體積＝  100ul × 檢測種類×2 。

◆   對于作某一個(gè)具體指標(biāo)來說，先用一系列稀釋度作一批標(biāo)本測定，得出對實(shí)驗(yàn)有意義的一個(gè)稀釋度（即測定劑量反應(yīng)曲線），然后用一個(gè)*適稀釋度測定即可。

◆   收集標(biāo)本前必須清楚要檢測的成份是否足夠穩(wěn)定。對收集后當(dāng)天進(jìn)行檢測的標(biāo)本，儲(chǔ)存在4℃?zhèn)溆�，如有特殊原因需要周期收集�?biāo)本，將標(biāo)本及時(shí)分裝后放在-20℃ 或-70℃ 條件下保存。避免反復(fù)凍融。標(biāo)本 2 -8 ℃ 可保存48 小時(shí).-20℃ 可保存1個(gè)月。 -70 度可保存6個(gè)月。部分激素類標(biāo)本需添加抑肽酶。

◆   血清標(biāo)本采集時(shí)，應(yīng)注意避免溶血，紅細(xì)胞溶解時(shí)會(huì)釋放出具有過氧化物酶活性的物質(zhì)，以 HRP為標(biāo)記的 ELISA 測定中，溶血標(biāo)本可能會(huì)增加非特異性顯色。

【人胰腺癌標(biāo)志物CA242ELISA試劑盒現(xiàn)貨】◆   為了保證尿液檢測結(jié)果的準(zhǔn)確性，必須正確收集尿液標(biāo)本和保存。盛尿容器要清潔干燥。使用一次性的容器（如塑料尿杯），避免因用藥并清洗不干凈而造成的污染，影響檢測結(jié)果。尿液標(biāo)本必須新鮮，留取后，應(yīng)及時(shí)檢測或保存，以免細(xì)菌繁殖。因在室溫中久置后（尤其是夏季）尿中磷酸鹽等可析出結(jié)晶而干擾檢測。

◆    標(biāo)本宜在新鮮時(shí)檢測。如有細(xì)菌污染，菌體中可能含有內(nèi)源性 HRP，也會(huì)產(chǎn)生假陽性反應(yīng)。保存過久可發(fā)生聚合在 ELISA中可使本底加深。

◆   凍結(jié)標(biāo)本融解后，蛋白質(zhì)局部濃縮，分布不均，應(yīng)充分混勻宜輕緩，避免氣泡，可上下顛倒混和，不要在混勻器上強(qiáng)烈振蕩。

◆   混濁或有沉淀的標(biāo)本應(yīng)先離心或過濾，澄清后再檢測。

◆   反復(fù)凍融會(huì)使蛋白效價(jià)降低，所以待測標(biāo)本如需保存作多次檢測，宜少量分裝冰存。也可加入適當(dāng)防腐劑。

◆   如果您在實(shí)驗(yàn)中遇到任何特殊問題，敬請咨詢 021-60348496標(biāo)本類型

ELISA常見的標(biāo)本一般包括：

◆   液體類標(biāo)本：包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清等。

◆   培養(yǎng)細(xì)胞

◆   組織標(biāo)本

這些標(biāo)本收集的時(shí)間、方法和保存都有一定的要求。

【人胰腺癌標(biāo)志物CA242ELISA試劑盒現(xiàn)貨】標(biāo)本的保存

一般說來，在 5 天內(nèi)測定的標(biāo)本可放置于 4℃，超過一周測定的需低溫凍存。

對收集后當(dāng)天進(jìn)行檢測的標(biāo)本，儲(chǔ)存在  4 ℃ 備用，如有特殊原因需要周期收集標(biāo)本， 將標(biāo)本及時(shí)分裝后放在  -20 ℃ 或  -70 ℃ 條件下保存。避免反復(fù)凍融。 標(biāo)本  2 -8 ℃ 可保存  48 小時(shí)，-20 ℃ 可保存  1 個(gè)月。-70 度可保存  6 個(gè)月。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類標(biāo)本需添加抑肽酶。標(biāo)本的處理 可用作 ELISA 測定的標(biāo)本十分廣泛，體液（如血清）、分泌物（唾液）和排泄物（如尿液）、細(xì)胞培養(yǎng)上清、細(xì)胞、組織等均可作標(biāo)本以測定其中某種成份。有些標(biāo)本可直接進(jìn)行測定，有些則需經(jīng)預(yù)處理。

◆ 血清：  

室溫血液自然凝固  10-20 分鐘后，離心  20 分鐘左右（  2000-3000 轉(zhuǎn)  / 分）。收集上清。如有沉淀形成，應(yīng)再次離心。  

◆ 血漿：  

應(yīng)根據(jù)試劑盒的要求選擇  EDTA 、檸檬酸鈉或肝素作為抗凝劑，加入  10 ％（  v/v ）抗凝劑 ( 0.1M 檸檬酸鈉或  1% heparin 或  2.0%EDTA.Na2) 混合  10-20 分鐘后，離心  20 分鐘左右（  2000-3000 轉(zhuǎn)  / 分）。仔細(xì)收集上清如有沉淀形 成，應(yīng)再次離心。  

【人胰腺癌標(biāo)志物CA242ELISA試劑盒現(xiàn)貨】◆ 尿液、胸腹水、腦脊液：  

用無菌管收集。離心  20 分鐘左右（  2000-3000 轉(zhuǎn)  / 分）。仔細(xì)收集上清。如有沉淀形成，應(yīng)再次離心。  

◆ 細(xì)胞培養(yǎng)上清：  

檢測分泌性的成份時(shí)，用無菌管收集。離心  20 分鐘左右（  2000-3000 轉(zhuǎn)  / 分）。仔細(xì)收集上清。檢測細(xì)胞內(nèi)的 成份時(shí)，用  PBS （  PH7.2-7.4 ）稀釋細(xì)胞懸液，細(xì)胞濃度達(dá)到  100 萬 /ml 左右。通過反復(fù)凍融，以使細(xì)胞破壞并放出細(xì)胞內(nèi)成份。離心  20 分鐘左右（  2000-3000 轉(zhuǎn) / 分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。  

◆ 細(xì)胞：

檢測細(xì)胞的成份時(shí)，對于貼壁細(xì)胞，去除培養(yǎng)液，用PBS、生理鹽水或無血清培養(yǎng)液洗一遍。加入適量裂解液。用槍吹打數(shù)下，使裂解液和細(xì)胞充分接觸。通常裂解液接觸細(xì)胞10 秒后，細(xì)胞就會(huì)被裂解。對于懸浮細(xì)胞，離心收集細(xì)胞，用PBS、生理鹽水或無血清培養(yǎng)液洗一遍.加入適量裂解液, 用槍吹打把細(xì)胞吹散。用手指輕彈以充分裂解細(xì)胞。充分裂解后應(yīng)沒有明顯的細(xì)胞沉淀。如果細(xì)胞量較多，必需分裝然后再裂解。充分裂解后，10000-14000g離心3-5 分鐘，取上

清，即可進(jìn)行后續(xù)操作。

【人胰腺癌標(biāo)志物CA242ELISA試劑盒現(xiàn)貨】◆ 組織標(biāo)本：  

切割標(biāo)本后，稱取重量。加入一定量的  PBS或組織蛋白萃取試劑，緩沖液中可加入蛋白酶抑制劑。用手工或勻漿器將標(biāo)本勻漿充分。離心  20 分鐘左右（  2000-3000 轉(zhuǎn)  / 分）。仔細(xì)收集上清置于  -20 度或  - 70 度保存，如有必要，可以將樣品濃縮干燥。分裝后一份待檢測，其余冷凍備用。齊一生物銷售：021－6034 8496；181214 53965；173021 04490  

PGS 周細(xì)胞生長因子    Pericyte Growth Supplement 5 ml                     

MenCGS 腦膜細(xì)胞生長因子     Meningeal Cell Growth Supplement      5 ml                     

NPrCGS  神經(jīng)前體細(xì)胞生長因子       Neural Precursor Cell Growth Supplement   5 ml                     

NGS       神經(jīng)元生長因子  Neuronal Growth Supplement       5 ml                     

NPCDS   神經(jīng)前體細(xì)胞分化生長因子       Neural Precursor Cell Differentiation Supplement 5 ml                     

OPCGS   少突膠質(zhì)前體細(xì)胞生長因子     Oligodendrocyte Precursor Cell Growth Supplement   5 ml                     

OGS       少突膠質(zhì)細(xì)胞生長因子     Oligodendrocyte Growth Supplement   5 ml                     

OPCDS   少突膠質(zhì)前體分化生長因子     Oligodendrocyte Precursor Cell Differentiation Supplement 5 ml                     

SCGS     雪旺細(xì)胞生長因子     Schwann Cell Growth Supplement       5 ml                     

AGS       星形細(xì)胞生長因子     Astrocyte Growth Supplement      5 ml                     

AGS-a     星形細(xì)胞生長因子-動(dòng)物    Astrocyte Growth Supplement-Animal  5 ml                     

MGS       小膠質(zhì)細(xì)胞生長因子    Microglia Growth Supplement      5 ml                     

MGS       巨噬細(xì)胞生長因子       Macrophage Growth Supplement  50 ml                          

KGS       角質(zhì)細(xì)胞生長因子     Keratinocyte Growth Supplement  5 ml                     

KGS-2    角質(zhì)細(xì)胞生長因子（不含 BPE)       BPE-free Keratinocyte Growth Supplement  5 ml                      【人胰腺癌標(biāo)志物CA242ELISA試劑盒現(xiàn)貨】

KGS-acf  角質(zhì)細(xì)胞生長因子（不含動(dòng)物成分）     Keratinocyte Growth Supplement-animal component free   5 ml                     

MelGS    黑色素細(xì)胞生長因子  Melanocyte Growth Supplement   5 ml                     

MelGS-2 黑色素細(xì)胞生長因子（不含TPA)   TPA-free Melanocyte Growth Supplement   5 ml                     

FGS 成纖維細(xì)胞生長因子    Fibroblast Growth Supplement      5 ml                     

FGS-sf    成纖維細(xì)胞生長因子-sf       Fibroblast Growth Supplement-sf  5 ml                     

FGS-acf  成纖維細(xì)胞生長因子（不含動(dòng)物成分）    Fibroblast Growth Supplement-animal component free       5 ml                     

FGS-2     成纖維細(xì)胞生長因子-2 Fibroblast Growth Supplement-2   5 ml                     

TEpiCGS 扁桃體上皮細(xì)胞生長因子    Tonsil Epithelial Cell Growth Supplement     5 ml                     

OKGS     口腔角質(zhì)細(xì)胞生長因子       Oral Keratinocyte Growth Supplement  5 ml                     

CoEpiCGSS    結(jié)腸上皮細(xì)胞生長因子       Colonic Epithelial Cell Growth Supplement   5 ml                     

BEpiCGS 支氣管上皮細(xì)胞生長因子  Bronchial Epithelial Cell Growth Supplement 5 ml                     

SAEpiCGS      小氣道上皮細(xì)胞生長因子  Small Airway Epithelial Cell Growth Supplement  5 ml                     

SkMCGS 骨骼肌細(xì)胞生長因子  Skeletal Muscle Cell Growth Supplement     5 ml                     

EpiCGS   上皮細(xì)胞生長因子     Epithelial Cell Growth Supplement 5 ml                     

EpiCGS-2       上皮細(xì)胞生長因子-2  Epithelial Cell Growth Supplement-2     5 ml                     

EpiCGS-a       動(dòng)物上皮細(xì)胞生長因子-2    Epithelial Cell Growth Supplement-animal     5 ml                      【人胰腺癌標(biāo)志物CA242ELISA試劑盒現(xiàn)貨】

MCGS    腎系膜細(xì)胞生長因子    Mesangial Cell Growth Supplement      5 ml                     

UCGS     尿道上皮細(xì)胞生長因子     Urothelial Cell Growth Supplement       5 ml                     

9240678  Bimba Rotary Actuator, AP           CNY                           

9240777  CE Update, AP196/98           CNY                           

9241773  RGQ, Installation    The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the Rotorgene-Q systems hardware ａnd trains the user on the operation of the Rotorgene software. After the training the user will be able to develop application programs, conduct a basic troubleshooting, ａnd understand the application output format.All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements.    CNY                           

9241763  QIAgility, Full Agreement      "Repair service delivery: onsite

Service response time (repair): 48 hours/2 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) or PC repair provision: not included

Transportation cost coverage: not applicable"      CNY                           

9241372  Reflector, UV lamp, EZ1a             CNY                           

9243545  QIAsymphony SP, PM Service     "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                           

9243045  Bracket, rear, transparent panel, Qsym         CNY                           

9242980  QIArent&go - Rent out fee QS RGQ System              CNY                            【人胰腺癌標(biāo)志物CA242ELISA試劑盒現(xiàn)貨】

9242764  QS RGQ, Premium Agreement      "Repair service delivery QIAsymphony SP/AS: onsite

Service response time (repair) QIAsymphony: 24 hours/1 business day

Repair service delivery RGQ: send to Regional Repair Center

Service response time (repair) RGQ: 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision RGQ: yes, included (provision time 2-3 days)

Transportation cost coverage: yes, full coverage

QIAsymphony Response Time starts when customer inquiry is received by local tech service ends when a service specialist is onsite.

- Inquiry Channel: Local Tech-Service Hotline, Mo.- Fr. 08:00-17:00 Uhr

- Call Cut-off Time: 12:00 am; on business days

- Customer Availability: maximum 2 hours phone trouble shooting to identify error ａnd spare parts

- QIAsense: Systems need to be connected to QIAsense for faster download of log files

- Software: QIAsymphony need to be updated with the latest software version

- Remote Area: 24 Hr onsite response only available for geographical areas within field service travel capability ａnd pre-12:00 delivery capability of spare parts"       CNY                           

9242750  Cooling adapter, PCR adapter, AS, Qsym            CNY                           

9242709  Validation Support, 2d, EZ1   The QIAGEN EZ1 Guided Validation Support service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the EZ1 system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, ａnd practical hands-on operation.  The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates. The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. CNY                           

9242699  QIAxcel, Full Agreement       "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery:

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: yes, included (provision time 2-3 days)

Transportation cost coverage: yes, full coverage" CNY                           

9242508  QIAsymphony SPAS, Premium Agreement   "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: not applicable

- QIAsense: Systems should to be connected to QIAsense for faster download of log files"     CNY                           

5000-1316      Rotary Shaker - Knob Locking             CNY                           

9241365  Grid, UV lamp, EZ1a            CNY                           

9240761  CPU, AP96            CNY                           

9240757  Downtube, 500 ml Bottle, AP96/196            CNY                           

9242768  Belt, x-drive, portal, SP, Qsym             CNY                           

9242715  Validation Support, 5d, EZ1   QThe QIAGEN EZ1 Accelerated Validation Support service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the EZ1 system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on user support ａnd EZ1 unit operation. The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates. The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Attendees will receive a certificate of participation in the hands-on exercises.     CNY                           

5000-1314      Rotary Shaker - Bar Roller (Pair)          CNY                           

5000-1305      Switch Power, SKR1            CNY                           

5000-1281      DML 2000 - Power Cord 220V B Type (DE)              CNY                           

5025-1015      Expand 4 Pipettor Stand        CNY                           

6000-3118      Plate Washer I - Wash Cap Assembly           CNY                            【人胰腺癌標(biāo)志物CA242ELISA試劑盒現(xiàn)貨】

6000-1203      96 Well Plate          CNY                           

5000-1418      Control Digene PCB, VTX2          CNY                           

5140-1220      hc2 GC-ID DNA Test           CNY                           

5050-1076 V.1       HYBRID CAPTURE SYSTEM             CNY                           

EA10324 EASYartus CMV RG PCR Kit 2           CNY                           

EA10223 EASYartus VZV RG_I           CNY                           

9240718  Tool, Calibration Weight, 20g, AP         CNY